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Improvement of pyruvate production based on regulation of intracellular redox state in engineered Escherichia coli
Yang, Maohua; Xing, Jianmin
2017-08-01
发表期刊BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
ISSN1226-8372
卷号22期号:4页码:376-381
摘要The commercial demand for pyruvate has been expanding. However, some challenges need to be overcome in the microbial production of pyruvate, such as low glucose consumption caused by excessive accumulation of NADH. In this study, weakening or block of the TCA cycle, overexpression of foreign NADH oxidase, and carbon sources with different oxidation state was attempted to decrease NADH accumulation in engineered strain YP211. Results showed that blocking or weakening TCA cycle could not lower the intracellular redox state in strain YP211.Overexpressing NADH oxidase from Lactococcus lactis significantly decreased the intracellular NADH content and increased the consumption rate of glucose. However, the yield of pyruvate did not increase significantly. Compared with glucose as carbon source, sodium gluconate with a higher oxidation state resulted in a significant decrease of NADH/NAD(+), and the concentration and yield of pyruvate increased by 62 and 6%, respectively. In the fed-batch fermentation, the yield of pyruvate increased to 0.78 g/g gluconate, and the concentration of pyruvate reached 78.8 g/L. It was suggested that sodium gluconate was a more ideal carbon source for strain YP211, which could effectively decrease NADH content and improve the pyruvate production.
关键词Escherichia Coli Pyruvate Redox State Nadh Oxidase Sodium Gluconate
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine
DOI10.1007/s12257-017-0061-z
收录类别SCI
语种英语
关键词[WOS]SACCHAROMYCES-CEREVISIAE ; GLYCOLYTIC FLUX ; NADH OXIDASE ; METABOLISM ; ACID ; DEHYDROGENASE ; EXPRESSION ; GLUCOSE ; DEMAND ; GROWTH
WOS研究方向Biotechnology & Applied Microbiology
WOS类目Biotechnology & Applied Microbiology
项目资助者National High Technology Research and Development Program of China(2014AA021900)
WOS记录号WOS:000410045300003
引用统计
文献类型期刊论文
条目标识符http://ir.ipe.ac.cn/handle/122111/23235
专题生化工程国家重点实验室
作者单位Chinese Acad Sci, Inst Proc Engn, Beijing 100190, Peoples R China
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Yang, Maohua,Xing, Jianmin. Improvement of pyruvate production based on regulation of intracellular redox state in engineered Escherichia coli[J]. BIOTECHNOLOGY AND BIOPROCESS ENGINEERING,2017,22(4):376-381.
APA Yang, Maohua,&Xing, Jianmin.(2017).Improvement of pyruvate production based on regulation of intracellular redox state in engineered Escherichia coli.BIOTECHNOLOGY AND BIOPROCESS ENGINEERING,22(4),376-381.
MLA Yang, Maohua,et al."Improvement of pyruvate production based on regulation of intracellular redox state in engineered Escherichia coli".BIOTECHNOLOGY AND BIOPROCESS ENGINEERING 22.4(2017):376-381.
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