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Improvement of pyruvate production based on regulation of intracellular redox state in engineered Escherichia coli
Yang, Maohua; Xing, Jianmin
2017-08-01
Source PublicationBIOTECHNOLOGY AND BIOPROCESS ENGINEERING
ISSN1226-8372
Volume22Issue:4Pages:376-381
Abstract

The commercial demand for pyruvate has been expanding. However, some challenges need to be overcome in the microbial production of pyruvate, such as low glucose consumption caused by excessive accumulation of NADH. In this study, weakening or block of the TCA cycle, overexpression of foreign NADH oxidase, and carbon sources with different oxidation state was attempted to decrease NADH accumulation in engineered strain YP211. Results showed that blocking or weakening TCA cycle could not lower the intracellular redox state in strain YP211.Overexpressing NADH oxidase from Lactococcus lactis significantly decreased the intracellular NADH content and increased the consumption rate of glucose. However, the yield of pyruvate did not increase significantly. Compared with glucose as carbon source, sodium gluconate with a higher oxidation state resulted in a significant decrease of NADH/NAD(+), and the concentration and yield of pyruvate increased by 62 and 6%, respectively. In the fed-batch fermentation, the yield of pyruvate increased to 0.78 g/g gluconate, and the concentration of pyruvate reached 78.8 g/L. It was suggested that sodium gluconate was a more ideal carbon source for strain YP211, which could effectively decrease NADH content and improve the pyruvate production.

KeywordEscherichia Coli Pyruvate Redox State Nadh Oxidase Sodium Gluconate
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1007/s12257-017-0061-z
Indexed BySCI
Language英语
WOS KeywordSaccharomyces-cerevisiae ; Glycolytic Flux ; Nadh Oxidase ; Metabolism ; Acid ; Dehydrogenase ; Expression ; Glucose ; Demand ; Growth
WOS Research AreaBiotechnology & Applied Microbiology
WOS SubjectBiotechnology & Applied Microbiology
Funding OrganizationNational High Technology Research and Development Program of China(2014AA021900)
WOS IDWOS:000410045300003
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/23235
Collection生化工程国家重点实验室
AffiliationChinese Acad Sci, Inst Proc Engn, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Yang, Maohua,Xing, Jianmin. Improvement of pyruvate production based on regulation of intracellular redox state in engineered Escherichia coli[J]. BIOTECHNOLOGY AND BIOPROCESS ENGINEERING,2017,22(4):376-381.
APA Yang, Maohua,&Xing, Jianmin.(2017).Improvement of pyruvate production based on regulation of intracellular redox state in engineered Escherichia coli.BIOTECHNOLOGY AND BIOPROCESS ENGINEERING,22(4),376-381.
MLA Yang, Maohua,et al."Improvement of pyruvate production based on regulation of intracellular redox state in engineered Escherichia coli".BIOTECHNOLOGY AND BIOPROCESS ENGINEERING 22.4(2017):376-381.
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