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Quorum sensing molecule-farnesol increased the production and biological activities of extracellular polysaccharide from Trametes versicolor
Wang, Ke-Feng1,3; Sui, Kun-Yan2; Guo, Chen1,3; Liu, Chun-Zhao1,2,3
2017-11-01
Source PublicationINTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
ISSN0141-8130
Volume104Issue:NOVPages:377-383
Abstract

A novel strategy of exposing 2-day-old mycelia cultures to 0.8 mM farnesol was developed to stimulate extracellular polysaccharide (EPS) production in Trametes versicolor submerged cultures. Farnesol, a quorum sensing molecule in fungi, could significantly increase EPS production by promoting polysaccharide biosynthesis and regulating mycelial morphology. EPS yield reached a maximum of 2.56 g/L that was 2.7-fold greater than that of control cultures. Farnesol made T. versicolor develop into fluffy, loose and multi-hyphae morphology, which facilitated the excretion of intracellular polysaccharide into culture medium. Moreover, EPS from farnesol-induced cultures (EPS-F) with higher carbohydrate and uronic acid contents mainly contained high molecular weight polysaccharide (134 kDa, 85%), and comprised glucose, mannose and galactose in a molar ratio of 34.2:2.1:1.0. These physicochemical properties led to stronger antioxidant and antitumor activities of EPS-F. This is the first report that farnesol can significantly improve the production of polysaccharide with higher biological activities. It provides a novel strategy to enhance the production and bioactivity of mushroom polysaccharide using microbial quorum sensing molecules. (C) 2017 Elsevier B.V. All rights reserved.

KeywordTrametes Versicolor Polysaccharide Farnesol Biological Activities
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI10.1016/j.ijbiomac.2017.06.053
Indexed BySCI
Language英语
WOS KeywordCORIOLUS-VERSICOLOR ; ANTIOXIDANT ACTIVITY ; GANODERMA-LUCIDUM ; SUBMERGED-CULTURE ; CANDIDA-ALBICANS ; MORPHOLOGY ; FERMENTATION ; PURIFICATION ; FUNGI ; POLYSACCHAROPEPTIDES
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry ; Polymer Science
WOS SubjectBiochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science
Funding OrganizationNational Basic Research Program (973 Program) of China(2013CB733600) ; National Natural Science Foundation of China(21476242) ; National Key Technology Research and Development Program of China(2015BAK45B01) ; National High Technology Research and Development Program (863 Program) of China(2012AA101803) ; Chinese Academy of Sciences(KSZD-EW-Z-015)
WOS IDWOS:000412959300042
Citation statistics
Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/23268
Collection生化工程国家重点实验室
Affiliation1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Qingdao Univ, Sch Mat Sci & Engn, Qingdao 266071, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Wang, Ke-Feng,Sui, Kun-Yan,Guo, Chen,et al. Quorum sensing molecule-farnesol increased the production and biological activities of extracellular polysaccharide from Trametes versicolor[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2017,104(NOV):377-383.
APA Wang, Ke-Feng,Sui, Kun-Yan,Guo, Chen,&Liu, Chun-Zhao.(2017).Quorum sensing molecule-farnesol increased the production and biological activities of extracellular polysaccharide from Trametes versicolor.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,104(NOV),377-383.
MLA Wang, Ke-Feng,et al."Quorum sensing molecule-farnesol increased the production and biological activities of extracellular polysaccharide from Trametes versicolor".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 104.NOV(2017):377-383.
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