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In Vivo Quantitation of Collagen/Gelatin Fragments Using HPLC-MS/MS Detection of Peptide Markers
Zhou, Wan1,2; Zhang, Guifeng3; Yin, Hui1; Liu, Lu1,2; Tang, Songshan1; Su, Zhiguo3; Liu, Tao1
2017-06-01
发表期刊NANOSCIENCE AND NANOTECHNOLOGY LETTERS
ISSN1941-4900
卷号9期号:6页码:945-950
摘要

Collagen/gelatin fragments are often polydisperse and heterogeneous, making them difficult for quantitation, especially during in vivo circulation. In this study, a new quantitation method was established based on the detection of collagen-specific peptide markers using HPLC-MS/MS. Blood samples were collected and digested for collagen-specific peptides identification and content determination after the infusion of bovine gelatin fragments into rabbits. Thirteen collagen-specific peptides, of which five were present in bovine collagen but absent in human collagen, were identified by MS detection and could be used as peptide markers for the quantitation of gelatin fragments in blood. One of the bovine collagen specific-peptides was then used as a peptide marker, and its detection linearity range and limit of quantification were confirmed. The pharmacokinetic parameters, such as half-life and elimination rate, and also the pharmacokinetic model of the infused gelatin fragments were determined from the time-concentration curve. This peptide marker based method offers better selectivity towards collagen/gelatin fragments quantitation and could find wider applications in the field of collagen/gelatin based products such as drug delivery materials, and nanoparticles.

关键词Collagen Gelatin Peptide Marker Mass Spectrometry
文章类型Article
WOS标题词Science & Technology ; Technology ; Physical Sciences
DOI10.1166/nnl.2017.2394
收录类别SCI
语种英语
关键词[WOS]Ionization-mass-spectrometry ; Recombinant Human Gelatin ; Identification ; Blood ; Quantification ; Chromatography ; Expression ; Prediction ; Products ; Bovine
WOS研究方向Science & Technology - Other Topics ; Materials Science ; Physics
WOS类目Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied
项目资助者Guangdong Provincial Science Technology Planning Project(2014A010107027) ; Natural Grant(2014A030313581 ; 2014A030313589 ; 2015A030313843)
WOS记录号WOS:000412946700018
引用统计
文献类型期刊论文
条目标识符http://ir.ipe.ac.cn/handle/122111/23277
专题生化工程国家重点实验室
作者单位1.Guangdong Pharmaceut Univ, Sch Basic Courses, Guangdong Key Lab Pharmaceut Bioact Subst, Guangzhou 510006, Guangdong, Peoples R China
2.Guangdong Pharmaceut Univ, Sch Pharm, Guangzhou 510006, Guangdong, Peoples R China
3.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100080, Peoples R China
推荐引用方式
GB/T 7714
Zhou, Wan,Zhang, Guifeng,Yin, Hui,et al. In Vivo Quantitation of Collagen/Gelatin Fragments Using HPLC-MS/MS Detection of Peptide Markers[J]. NANOSCIENCE AND NANOTECHNOLOGY LETTERS,2017,9(6):945-950.
APA Zhou, Wan.,Zhang, Guifeng.,Yin, Hui.,Liu, Lu.,Tang, Songshan.,...&Liu, Tao.(2017).In Vivo Quantitation of Collagen/Gelatin Fragments Using HPLC-MS/MS Detection of Peptide Markers.NANOSCIENCE AND NANOTECHNOLOGY LETTERS,9(6),945-950.
MLA Zhou, Wan,et al."In Vivo Quantitation of Collagen/Gelatin Fragments Using HPLC-MS/MS Detection of Peptide Markers".NANOSCIENCE AND NANOTECHNOLOGY LETTERS 9.6(2017):945-950.
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