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In Vivo Quantitation of Collagen/Gelatin Fragments Using HPLC-MS/MS Detection of Peptide Markers
Zhou, Wan1,2; Zhang, Guifeng3; Yin, Hui1; Liu, Lu1,2; Tang, Songshan1; Su, Zhiguo3; Liu, Tao1
2017-06-01
Source PublicationNANOSCIENCE AND NANOTECHNOLOGY LETTERS
ISSN1941-4900
Volume9Issue:6Pages:945-950
Abstract

Collagen/gelatin fragments are often polydisperse and heterogeneous, making them difficult for quantitation, especially during in vivo circulation. In this study, a new quantitation method was established based on the detection of collagen-specific peptide markers using HPLC-MS/MS. Blood samples were collected and digested for collagen-specific peptides identification and content determination after the infusion of bovine gelatin fragments into rabbits. Thirteen collagen-specific peptides, of which five were present in bovine collagen but absent in human collagen, were identified by MS detection and could be used as peptide markers for the quantitation of gelatin fragments in blood. One of the bovine collagen specific-peptides was then used as a peptide marker, and its detection linearity range and limit of quantification were confirmed. The pharmacokinetic parameters, such as half-life and elimination rate, and also the pharmacokinetic model of the infused gelatin fragments were determined from the time-concentration curve. This peptide marker based method offers better selectivity towards collagen/gelatin fragments quantitation and could find wider applications in the field of collagen/gelatin based products such as drug delivery materials, and nanoparticles.

KeywordCollagen Gelatin Peptide Marker Mass Spectrometry
SubtypeArticle
WOS HeadingsScience & Technology ; Technology ; Physical Sciences
DOI10.1166/nnl.2017.2394
Indexed BySCI
Language英语
WOS KeywordIonization-mass-spectrometry ; Recombinant Human Gelatin ; Identification ; Blood ; Quantification ; Chromatography ; Expression ; Prediction ; Products ; Bovine
WOS Research AreaScience & Technology - Other Topics ; Materials Science ; Physics
WOS SubjectNanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied
Funding OrganizationGuangdong Provincial Science Technology Planning Project(2014A010107027) ; Natural Grant(2014A030313581 ; 2014A030313589 ; 2015A030313843)
WOS IDWOS:000412946700018
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/23277
Collection生化工程国家重点实验室
Affiliation1.Guangdong Pharmaceut Univ, Sch Basic Courses, Guangdong Key Lab Pharmaceut Bioact Subst, Guangzhou 510006, Guangdong, Peoples R China
2.Guangdong Pharmaceut Univ, Sch Pharm, Guangzhou 510006, Guangdong, Peoples R China
3.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Zhou, Wan,Zhang, Guifeng,Yin, Hui,et al. In Vivo Quantitation of Collagen/Gelatin Fragments Using HPLC-MS/MS Detection of Peptide Markers[J]. NANOSCIENCE AND NANOTECHNOLOGY LETTERS,2017,9(6):945-950.
APA Zhou, Wan.,Zhang, Guifeng.,Yin, Hui.,Liu, Lu.,Tang, Songshan.,...&Liu, Tao.(2017).In Vivo Quantitation of Collagen/Gelatin Fragments Using HPLC-MS/MS Detection of Peptide Markers.NANOSCIENCE AND NANOTECHNOLOGY LETTERS,9(6),945-950.
MLA Zhou, Wan,et al."In Vivo Quantitation of Collagen/Gelatin Fragments Using HPLC-MS/MS Detection of Peptide Markers".NANOSCIENCE AND NANOTECHNOLOGY LETTERS 9.6(2017):945-950.
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