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Improved production and antitumor activity of intracellular protein-polysaccharide from Trametes versicolor by the quorum sensing molecule-tyrosol
Wang, Ke-Feng1,3; Sui, Kun-yan2; Guo, Chen1; Liu, Chun-Zhao1,2
2017-10-01
Source PublicationJOURNAL OF FUNCTIONAL FOODS
ISSN1756-4646
Volume37Issue:OCTPages:90-96
Abstract

Tyrosol, a quorum sensing molecule in fungi, significantly improved intracellular protein-polysaccharide (IPS) production by promoting cell growth and stimulating IPS biosynthesis in Trametes versicolor submerged cultures. IPS production was increased to 390 mg/L in tyrosol-induced cultures under optimal concentration (5 mM), which was 1.95-fold higher than that of control cultures. Moreover, IPS from tyrosol-induced cultures (IPS-T) with higher carbohydrate and protein contents mainly contained high molecular weight polysaccharide (97 kDa, 70%), and composed of glucose, mannose and galactose in a molar ratio of 14.8:2.4:1.0. These physicochemical properties led to its stronger antitumor activity. The anti-proliferative mechanism was identified as cell cycle arrest with cell accumulation in S phase and increase in apoptosis. Herein, it is the first report that tyrosol can improve the production of IPS with better antitumor activity. Together these results bring new opportunities in increasing the production and bioactivity of mushroom polysaccharide using microbial quorum sensing molecules. (C) 2017 Elsevier Ltd. All rights reserved.

KeywordIntracellular Protein-polysaccharide Trametes Versicolor Tyrosol Physicochemical Properties Antitumor Activity
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1016/j.jff.2017.07.034
Indexed BySCI
Language英语
WOS KeywordCORIOLUS-VERSICOLOR ; CANDIDA-ALBICANS ; MYCELIAL FERMENTATION ; ANTIOXIDANT ACTIVITY ; SUBMERGED-CULTURE ; MUSHROOMS ; POLYSACCHAROPEPTIDES ; PROLIFERATION ; BIOACTIVITIES ; PURIFICATION
WOS Research AreaFood Science & Technology
WOS SubjectFood Science & Technology
Funding OrganizationNational Basic Research Program (973 Program) of China(2013CB733600) ; National Natural Science Foundation of China(21476242) ; National Key Technology Research and Development Program of China(2015BAK45801) ; National High Technology Research and Development Program (863 Program) of China(2012AA101803) ; Knowledge Innovation Program of the Chinese Academy of Sciences(KSZD-EW-Z-015)
WOS IDWOS:000412863400010
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/23278
Collection生化工程国家重点实验室
Affiliation1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Qingdao Univ, Sch Mat Sci & Engn, Collaborat Innovat Ctr Marine Biomass Fibers Mat, Qingdao 266071, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Wang, Ke-Feng,Sui, Kun-yan,Guo, Chen,et al. Improved production and antitumor activity of intracellular protein-polysaccharide from Trametes versicolor by the quorum sensing molecule-tyrosol[J]. JOURNAL OF FUNCTIONAL FOODS,2017,37(OCT):90-96.
APA Wang, Ke-Feng,Sui, Kun-yan,Guo, Chen,&Liu, Chun-Zhao.(2017).Improved production and antitumor activity of intracellular protein-polysaccharide from Trametes versicolor by the quorum sensing molecule-tyrosol.JOURNAL OF FUNCTIONAL FOODS,37(OCT),90-96.
MLA Wang, Ke-Feng,et al."Improved production and antitumor activity of intracellular protein-polysaccharide from Trametes versicolor by the quorum sensing molecule-tyrosol".JOURNAL OF FUNCTIONAL FOODS 37.OCT(2017):90-96.
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