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Structural, functional and physiochemical properties of dextran-bovine hemoglobin conjugate as a hemoglobin-based oxygen carrier
Wang, Ying1; Zhang, Shan1,3; Zhang, Jun1; Yu, Wei-Li2; Gao, Da-Wei3; Wang, Quan1; You, Guo-Xing1; Hu, Tao2; Zhao, Lian1; Zhou, Hong1
2017-09-01
Source PublicationPROCESS BIOCHEMISTRY
ISSN1359-5113
Volume60Pages:67-73
Abstract

As a hemoglobin (Hb)-based oxygen carrier (HBOC), Hb suffers from the disadvantages of short half-life, renal toxicity and vasoactivity. Because dextran is a macromolecule that can be easily derivatized with various chemical moieties, conjugation of Hb with dextran can effectively increase the size of Hb and overcome the disadvantages of Hb. Thus, a dextran-bovine Hb (bHb) conjugate (dex-bHb) was prepared by conjugation of bHb with periodate-oxidized dextran here. As an important functional amino acid residue of bHb, Cys-93(beta) was reversibly protected by 4,4 '-dithiodipyridine to avoid reaction with periodate-oxidized dextran. Dex-bHb showed significantly higher hydrodynamic volume and higher viscosity than bHb. Conjugation with dextran stabilized the R state of bHb and slightly altered the heme environment of bHb. Conjugation with dextran decreased the P-50 of bHb, lowered the sensitivity to the allosteric effectors and slightly decreased the autoxidation rate of bHb. Thus, dex-bHb was expected to act as a potent HBOC with low oxidative toxicity.

KeywordHemoglobin-based Oxygen Carriers Dextran Bovine Hemoglobin Conjugation Oxygen Affinity
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Technology
DOI10.1016/j.procbio.2017.05.021
Indexed BySCI
Language英语
WOS KeywordPegylated Hemoglobin ; Binding-properties ; Blood Replacement ; Soluble Dextran ; Guinea-pig ; Peg-hb ; Heme ; Cys-93(Beta) ; Recombinant ; Mp4
WOS Research AreaBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering
WOS SubjectBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering, Chemical
Funding OrganizationNational Natural Science Foundation of China(31271001) ; National High Technology Research and Development Program of China(2012AA021902)
WOS IDWOS:000413377400009
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Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/23288
Collection生化工程国家重点实验室
Affiliation1.Acad Mil Med Sci, Inst Transfus Med, Beijing 100850, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
3.Yanshan Univ, Coll Environm & Chem Engn, Dept Biochem Engn, Qinhuangdao 066004, Hebei, Peoples R China
Recommended Citation
GB/T 7714
Wang, Ying,Zhang, Shan,Zhang, Jun,et al. Structural, functional and physiochemical properties of dextran-bovine hemoglobin conjugate as a hemoglobin-based oxygen carrier[J]. PROCESS BIOCHEMISTRY,2017,60:67-73.
APA Wang, Ying.,Zhang, Shan.,Zhang, Jun.,Yu, Wei-Li.,Gao, Da-Wei.,...&Zhou, Hong.(2017).Structural, functional and physiochemical properties of dextran-bovine hemoglobin conjugate as a hemoglobin-based oxygen carrier.PROCESS BIOCHEMISTRY,60,67-73.
MLA Wang, Ying,et al."Structural, functional and physiochemical properties of dextran-bovine hemoglobin conjugate as a hemoglobin-based oxygen carrier".PROCESS BIOCHEMISTRY 60(2017):67-73.
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