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Conjugation of the CRM197-inulin conjugate significantly increases the immunogenicity of Mycobacterium tuberculosis CFP10-TB10.4 fusion protein
Hua, Shun1,2,3; Yu, Weili2; Hu, Chunyang2; Wei, Dong2; Shen, Lijuan2; Hu, Tao2; Yi, Youjin1
2017-11-01
发表期刊BIOORGANIC & MEDICINAL CHEMISTRY
ISSN0968-0896
卷号25期号:21页码:5968-5974
摘要

Mycobacterium tuberculosis (Mtb) is a serious fatal pathogen that causes tuberculosis (TB). Effective vaccination is urgently needed to deal with the serious threat from TB. Mtb-secreted protein antigens are important virulence determinants of Mtb with poor immunogenicity. Adjuvants and antigen delivery systems are thus highly desired to improve the immunogenicity of protein antigens. Inulin is a biocompatible polysaccharide (PS) adjuvant that can stimulate a strong cellular and humoral immunity. Bacterial capsular PS and haptens have been conjugated with cross-reacting material 197 (CRM197) to improve their immunogenicity. CFP10 and TB10.4 were two Mtb-secreted immunodominant protein antigens. A CFP10-TB10.4 fusion protein (CT) was used as the antigen for covalent conjugation with the CRM197-inulin conjugate (CRM-inu). The resultant conjugate (CT-CRM-inu) elicited high CT-specific IgG titers, stimulated splenocyte proliferation and provoked the secretion of Th1-type and Th2-type cytokines. Conjugation with CRM-inu significantly prolonged the systemic circulation of CT and exposure to the immune system. Moreover, CT-CRM-inu showed no apparent toxicity to cardiac, hepatic and renal functions. Thus, conjugation of CT with CRM-inu provided an effective strategy for development of protein-based vaccines against Mtb infection. (C) 2017 Elsevier Ltd. All rights reserved.

关键词M. Tuberculosis Conjugation Inulin Crm197 Adjuvant Subunit Vaccine
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI10.1016/j.bmc.2017.09.027
收录类别SCI
语种英语
关键词[WOS]Vaccine Development ; Polysaccharide Adjuvant ; Influenza Vaccine ; Advax(Tm) ; Delivery ; Antigen ; Inulin ; Cell
WOS研究方向Biochemistry & Molecular Biology ; Pharmacology & Pharmacy ; Chemistry
WOS类目Biochemistry & Molecular Biology ; Chemistry, Medicinal ; Chemistry, Organic
项目资助者National Natural Science Foundation of China(81703445 ; Beijing Natural Science Foundation(7142104) ; Science and Technology Service Network Initiative Project of Chinese Academy of Sciences(KFJEW-STS-027 ; 81700181 ; KFJ-EW-STS-098) ; 81402861)
WOS记录号WOS:000413755600008
引用统计
文献类型期刊论文
条目标识符http://ir.ipe.ac.cn/handle/122111/23371
专题生化工程国家重点实验室
作者单位1.Hunan Agr Univ, Coll Food Sci & Technol, Changsha 410128, Hunan, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, 1 Bei Er Tiao St, Beijing 100190, Peoples R China
3.Xiangnan Univ, Sch Publ Hlth, Chenzhou 423000, Hunan, Peoples R China
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GB/T 7714
Hua, Shun,Yu, Weili,Hu, Chunyang,et al. Conjugation of the CRM197-inulin conjugate significantly increases the immunogenicity of Mycobacterium tuberculosis CFP10-TB10.4 fusion protein[J]. BIOORGANIC & MEDICINAL CHEMISTRY,2017,25(21):5968-5974.
APA Hua, Shun.,Yu, Weili.,Hu, Chunyang.,Wei, Dong.,Shen, Lijuan.,...&Yi, Youjin.(2017).Conjugation of the CRM197-inulin conjugate significantly increases the immunogenicity of Mycobacterium tuberculosis CFP10-TB10.4 fusion protein.BIOORGANIC & MEDICINAL CHEMISTRY,25(21),5968-5974.
MLA Hua, Shun,et al."Conjugation of the CRM197-inulin conjugate significantly increases the immunogenicity of Mycobacterium tuberculosis CFP10-TB10.4 fusion protein".BIOORGANIC & MEDICINAL CHEMISTRY 25.21(2017):5968-5974.
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