Switch on a more efficient pyruvate synthesis pathway based on transcriptome analysis and metabolic evolution
Yang, Maohua1; Chen, Ruonan2; Mu, Tingzhen1; Zhang, Xiang3; Xing, Jianmin1
2017-11-01
发表期刊JOURNAL OF BIOSCIENCE AND BIOENGINEERING
ISSN1389-1723
卷号124期号:5页码:523-527
摘要

Due to the decrease of intracellular NADH availability, gluconate metabolism is more conducive to pyruvate production than glucose. Transcriptome analysis revealed that the Entner-Doudoroff (ED) pathway was activated by gluconate in Escherichia coli YP211 (MG1655 Delta IdhA Delta pflB Delta pta-ackA Delta poxB Delta ppc Delta frdBC). To construct a new pyruvate producing strain with glucose metabolism via ED pathway, the genes ppsA, ptsG, pgi and gnd were deleted sequentially to reduce the demand for PEP and block the Embden-Meyerhor-Parnas pathway and Pentose-Phosphate pathway. After nearly 1000 generations of growth-based selection, the evolved strain YP404 was isolated and the ED pathway was proved to be activated as the primary glycolytic pathway. Comparing with YP211, the pyruvate concentration and yield increased by 59% and 10.1%, respectively. In fed-batch fermentation, the pyruvate concentration reached 83.5 gl(-1) with a volumetric productivity of 2.3 gl(-1) h(-1). This was the first time to produce pyruvate via ED pathway, and prove that this was a more effective way. (C) 2017, The Society for Biotechnology, Japan. All rights reserved.

关键词Pyruvate Transcriptome Entner-doudoroff Pathway Metabolic Evolution Redox State
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine
DOI10.1016/j.jbiosc.2017.06.004
收录类别SCI
语种英语
关键词[WOS]Escherichia-coli ; Biotechnological Production ; Torulopsis-glabrata ; Dehydrogenase ; Productivity ; Enhancement ; Succinate ; Glucose
WOS研究方向Biotechnology & Applied Microbiology ; Food Science & Technology
WOS类目Biotechnology & Applied Microbiology ; Food Science & Technology
项目资助者Tianjin Key Lab of Industrial Microbiology (Tianjin University of Science Technology) ; Foundation of Key Laboratory of Industrial Fermentation Microbiology of Ministry of Education(2015IM002) ; National High Technology Research and Development Program of China(2014AA021900)
WOS记录号WOS:000414620300006
引用统计
文献类型期刊论文
条目标识符http://ir.ipe.ac.cn/handle/122111/23388
专题湿法冶金清洁生产技术国家工程实验室
作者单位1.Chinese Acad Sci, Inst Proc Engn, Key Lab Green Proc & Engn, Beijing 100190, Peoples R China
2.Beijing Univ Chem Technol, Beijing 100029, Peoples R China
3.Shandong Acad Agr Sci, Inst Agrofood Sci & Technol, 202 North Ind Rd, Jinan 250100, Shandong, Peoples R China
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Yang, Maohua,Chen, Ruonan,Mu, Tingzhen,et al. Switch on a more efficient pyruvate synthesis pathway based on transcriptome analysis and metabolic evolution[J]. JOURNAL OF BIOSCIENCE AND BIOENGINEERING,2017,124(5):523-527.
APA Yang, Maohua,Chen, Ruonan,Mu, Tingzhen,Zhang, Xiang,&Xing, Jianmin.(2017).Switch on a more efficient pyruvate synthesis pathway based on transcriptome analysis and metabolic evolution.JOURNAL OF BIOSCIENCE AND BIOENGINEERING,124(5),523-527.
MLA Yang, Maohua,et al."Switch on a more efficient pyruvate synthesis pathway based on transcriptome analysis and metabolic evolution".JOURNAL OF BIOSCIENCE AND BIOENGINEERING 124.5(2017):523-527.
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