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Switch on a more efficient pyruvate synthesis pathway based on transcriptome analysis and metabolic evolution
Yang, Maohua1; Chen, Ruonan2; Mu, Tingzhen1; Zhang, Xiang3; Xing, Jianmin1
2017-11-01
Source PublicationJOURNAL OF BIOSCIENCE AND BIOENGINEERING
ISSN1389-1723
Volume124Issue:5Pages:523-527
Abstract

Due to the decrease of intracellular NADH availability, gluconate metabolism is more conducive to pyruvate production than glucose. Transcriptome analysis revealed that the Entner-Doudoroff (ED) pathway was activated by gluconate in Escherichia coli YP211 (MG1655 Delta IdhA Delta pflB Delta pta-ackA Delta poxB Delta ppc Delta frdBC). To construct a new pyruvate producing strain with glucose metabolism via ED pathway, the genes ppsA, ptsG, pgi and gnd were deleted sequentially to reduce the demand for PEP and block the Embden-Meyerhor-Parnas pathway and Pentose-Phosphate pathway. After nearly 1000 generations of growth-based selection, the evolved strain YP404 was isolated and the ED pathway was proved to be activated as the primary glycolytic pathway. Comparing with YP211, the pyruvate concentration and yield increased by 59% and 10.1%, respectively. In fed-batch fermentation, the pyruvate concentration reached 83.5 gl(-1) with a volumetric productivity of 2.3 gl(-1) h(-1). This was the first time to produce pyruvate via ED pathway, and prove that this was a more effective way. (C) 2017, The Society for Biotechnology, Japan. All rights reserved.

KeywordPyruvate Transcriptome Entner-doudoroff Pathway Metabolic Evolution Redox State
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1016/j.jbiosc.2017.06.004
Indexed BySCI
Language英语
WOS KeywordEscherichia-coli ; Biotechnological Production ; Torulopsis-glabrata ; Dehydrogenase ; Productivity ; Enhancement ; Succinate ; Glucose
WOS Research AreaBiotechnology & Applied Microbiology ; Food Science & Technology
WOS SubjectBiotechnology & Applied Microbiology ; Food Science & Technology
Funding OrganizationTianjin Key Lab of Industrial Microbiology (Tianjin University of Science Technology) ; Foundation of Key Laboratory of Industrial Fermentation Microbiology of Ministry of Education(2015IM002) ; National High Technology Research and Development Program of China(2014AA021900)
WOS IDWOS:000414620300006
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/23388
Collection湿法冶金清洁生产技术国家工程实验室
Affiliation1.Chinese Acad Sci, Inst Proc Engn, Key Lab Green Proc & Engn, Beijing 100190, Peoples R China
2.Beijing Univ Chem Technol, Beijing 100029, Peoples R China
3.Shandong Acad Agr Sci, Inst Agrofood Sci & Technol, 202 North Ind Rd, Jinan 250100, Shandong, Peoples R China
Recommended Citation
GB/T 7714
Yang, Maohua,Chen, Ruonan,Mu, Tingzhen,et al. Switch on a more efficient pyruvate synthesis pathway based on transcriptome analysis and metabolic evolution[J]. JOURNAL OF BIOSCIENCE AND BIOENGINEERING,2017,124(5):523-527.
APA Yang, Maohua,Chen, Ruonan,Mu, Tingzhen,Zhang, Xiang,&Xing, Jianmin.(2017).Switch on a more efficient pyruvate synthesis pathway based on transcriptome analysis and metabolic evolution.JOURNAL OF BIOSCIENCE AND BIOENGINEERING,124(5),523-527.
MLA Yang, Maohua,et al."Switch on a more efficient pyruvate synthesis pathway based on transcriptome analysis and metabolic evolution".JOURNAL OF BIOSCIENCE AND BIOENGINEERING 124.5(2017):523-527.
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