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Facile and green fabrication of cation exchange membrane adsorber with unprecedented adsorption capacity for protein purification
Khan, M. Kamran1,2; Luo, Jianquan1,2; Khan, Rashid1,2; Fan, Jinxin1,2; Wan, Yinhua1,2
2017-10-27
Source PublicationJOURNAL OF CHROMATOGRAPHY A
ISSN0021-9673
Volume1521Pages:19-26
Abstract

Fabricating membrane adsorbers with high adsorption capacity and appreciable throughput for the separation and purification of protein products is challenging in biomedical and pharmaceutical industries. Herein, we report the synthesis of a novel membrane adsorber by functionalizing a nylon microfiltration membrane with alginate dialdehyde (ADA) followed by sulphonic addition, without any solvent usage, and its successful application in the purification of lysozyme. Taking advantage of abundant dual cation exchange (CEX) groups on sulphonic-ADA (S-ADA) ligands, this novel S-ADA-nylon membrane adsorber showed an unprecedented static binding capicity of 286 mg/mL for lysozyme adsorption. Meanwhile, the prepared membrane adsorber could be easily regenerated (complete protein elution) under mild conditions and be reused at least for five times. Featured with a unique selectivity, the S-ADA-nylon membrane also captured lysozyme from chicken egg white solution with a high purity (100%) and a high recovery of 98%. The purified lysozyme showed similar specific activity as commercial product. The present work provides a facile, green and low-cost approach for the preparation of high-performance membrane adsorbers, which has a great potential in protein production. (C) 2017 Elsevier B.V. All rights reserved.

KeywordMembrane Chromatography Alginate Dialdehyde Lysozyme Protein Purification Microfiltration
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI10.1016/j.chroma.2017.09.031
Indexed BySCI
Language英语
WOS KeywordMonoclonal-antibody ; Step-purification ; Chromatography ; Separation ; Lysozyme ; Clearance ; Cryogel ; Design ; Virus ; Beads
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
Funding OrganizationNational Science Foundation of China(21506229) ; Youth Innovation Promotion Association of Chinese Academy of Sciences(2017069) ; '100 Talents Program' of Chinese Academy of Sciences
WOS IDWOS:000413382800003
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/23411
Collection生化工程国家重点实验室
Affiliation1.Chinese Acad Sci, State Key Lab Biochem Engn, Inst Proc Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Khan, M. Kamran,Luo, Jianquan,Khan, Rashid,et al. Facile and green fabrication of cation exchange membrane adsorber with unprecedented adsorption capacity for protein purification[J]. JOURNAL OF CHROMATOGRAPHY A,2017,1521:19-26.
APA Khan, M. Kamran,Luo, Jianquan,Khan, Rashid,Fan, Jinxin,&Wan, Yinhua.(2017).Facile and green fabrication of cation exchange membrane adsorber with unprecedented adsorption capacity for protein purification.JOURNAL OF CHROMATOGRAPHY A,1521,19-26.
MLA Khan, M. Kamran,et al."Facile and green fabrication of cation exchange membrane adsorber with unprecedented adsorption capacity for protein purification".JOURNAL OF CHROMATOGRAPHY A 1521(2017):19-26.
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