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Facile and green fabrication of cation exchange membrane adsorber with unprecedented adsorption capacity for protein purification
Khan, M. Kamran1,2; Luo, Jianquan1,2; Khan, Rashid1,2; Fan, Jinxin1,2; Wan, Yinhua1,2
2017-10-27
发表期刊JOURNAL OF CHROMATOGRAPHY A
ISSN0021-9673
卷号1521页码:19-26
摘要

Fabricating membrane adsorbers with high adsorption capacity and appreciable throughput for the separation and purification of protein products is challenging in biomedical and pharmaceutical industries. Herein, we report the synthesis of a novel membrane adsorber by functionalizing a nylon microfiltration membrane with alginate dialdehyde (ADA) followed by sulphonic addition, without any solvent usage, and its successful application in the purification of lysozyme. Taking advantage of abundant dual cation exchange (CEX) groups on sulphonic-ADA (S-ADA) ligands, this novel S-ADA-nylon membrane adsorber showed an unprecedented static binding capicity of 286 mg/mL for lysozyme adsorption. Meanwhile, the prepared membrane adsorber could be easily regenerated (complete protein elution) under mild conditions and be reused at least for five times. Featured with a unique selectivity, the S-ADA-nylon membrane also captured lysozyme from chicken egg white solution with a high purity (100%) and a high recovery of 98%. The purified lysozyme showed similar specific activity as commercial product. The present work provides a facile, green and low-cost approach for the preparation of high-performance membrane adsorbers, which has a great potential in protein production. (C) 2017 Elsevier B.V. All rights reserved.

关键词Membrane Chromatography Alginate Dialdehyde Lysozyme Protein Purification Microfiltration
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI10.1016/j.chroma.2017.09.031
收录类别SCI
语种英语
关键词[WOS]Monoclonal-antibody ; Step-purification ; Chromatography ; Separation ; Lysozyme ; Clearance ; Cryogel ; Design ; Virus ; Beads
WOS研究方向Biochemistry & Molecular Biology ; Chemistry
WOS类目Biochemical Research Methods ; Chemistry, Analytical
项目资助者National Science Foundation of China(21506229) ; Youth Innovation Promotion Association of Chinese Academy of Sciences(2017069) ; '100 Talents Program' of Chinese Academy of Sciences
WOS记录号WOS:000413382800003
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ipe.ac.cn/handle/122111/23411
专题生化工程国家重点实验室
作者单位1.Chinese Acad Sci, State Key Lab Biochem Engn, Inst Proc Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
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Khan, M. Kamran,Luo, Jianquan,Khan, Rashid,et al. Facile and green fabrication of cation exchange membrane adsorber with unprecedented adsorption capacity for protein purification[J]. JOURNAL OF CHROMATOGRAPHY A,2017,1521:19-26.
APA Khan, M. Kamran,Luo, Jianquan,Khan, Rashid,Fan, Jinxin,&Wan, Yinhua.(2017).Facile and green fabrication of cation exchange membrane adsorber with unprecedented adsorption capacity for protein purification.JOURNAL OF CHROMATOGRAPHY A,1521,19-26.
MLA Khan, M. Kamran,et al."Facile and green fabrication of cation exchange membrane adsorber with unprecedented adsorption capacity for protein purification".JOURNAL OF CHROMATOGRAPHY A 1521(2017):19-26.
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