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A novel polymer-grafted cation exchanger for high-capacity protein chromatography: The role of polymer architecture
Zhang, Su-Ling1,2,3; Zhao, Ming2,3; Yang, Wei2,3; Luo, Jian1; Sun, Yan2,3; Shi, Qing-Hong1,2,3
2017-12-15
Source PublicationBIOCHEMICAL ENGINEERING JOURNAL
ISSN1369-703X
Volume128Issue:DECPages:218-227
Abstract

In this work, a branched polymer-grafted cation exchanger was synthesized on a Sepharose FF matrix via atom transfer radical polymerization using 3-sulfopropyl methacrylate potassium salt (SPM) as a functional monomer and 2-(2-bromo-isobutyryloxy)ethyl methacrylate as a branching monomer. The resulting branched poly(SPM)-grafted cation exchangers exhibit typical ionic exchange characteristics for lysozyme adsorption and the maximum adsorption capacity reach 450 mgimL on Sep-BrS-S12B2 whereas gamma-globulin adsorption is more dependent on polymer architecture. By adjusting the ratio of the monomers, very high adsorption capacity for lysozyme and gamma-globulin can be achieved in the more branched Sep-BrL-S4B3 while gamma-globulin has a much higher effective diffusivity in Sep-BrL-S4B3. Experimental evidences show that the performance of polymer -grafted cation exchanger can be improved by regulating polymer architecture. The calorimetric results further indicate that counter-ions are released from polymer and proteins during protein adsorption. With an increase of the adsorption density, adsorbed proteins experience a change of molecular orientation along poly(SPM) chain. In Sep-BrL-S4B3, dynamic binding capacities reached 145 mgimL for lysozyme and 96 mgimL for gamma-globulin, demonstrating that Sep-BrL-S4B3 is a promising type of novel high-capacity cation exchangers. This research gives clue to the design of high-capacity cation exchangers and offers insights into protein adsorption on polymer -grafted cation exchangers. (C) 2017 Elsevier B.V. All rights reserved.

KeywordHigh-capacity Adsorption Cation Exchanger Branched Poly(3-sulfopropyl Methacrylate) Atom Transfer Radical Polymerization Gamma-globulin Molecular Orientation
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Technology
DOI10.1016/j.bej.2017.10.006
Indexed BySCI
Language英语
WOS KeywordTransfer Radical Polymerization ; Pore-size Distributions ; Ion-exchange ; Sepharose Ff ; Monoclonal-antibodies ; Agarose Media ; Adsorption ; Resins ; Adsorbents ; Transport
WOS Research AreaBiotechnology & Applied Microbiology ; Engineering
WOS SubjectBiotechnology & Applied Microbiology ; Engineering, Chemical
Funding OrganizationNatural Science Foundation of China(21476166 ; Open Funding Project of the State Key Laboratory of Biochemical Engineering(2014KF-03) ; Tianjin Natural Science Foundation(15JCYBJC48500) ; 21236005)
WOS IDWOS:000417008900025
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/23482
Collection研究所(批量导入)
Corresponding AuthorShi, Qing-Hong
Affiliation1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 10090, Peoples R China
2.Tianjin Univ, Sch Chem Engn & Technol, Dept Biochem Engn, Tianjin 300350, Peoples R China
3.Tianjin Univ, Sch Chem Engn & Technol, Minist Educ, Key Lab Syst Bioengn, Tianjin 300350, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Su-Ling,Zhao, Ming,Yang, Wei,et al. A novel polymer-grafted cation exchanger for high-capacity protein chromatography: The role of polymer architecture[J]. BIOCHEMICAL ENGINEERING JOURNAL,2017,128(DEC):218-227.
APA Zhang, Su-Ling,Zhao, Ming,Yang, Wei,Luo, Jian,Sun, Yan,&Shi, Qing-Hong.(2017).A novel polymer-grafted cation exchanger for high-capacity protein chromatography: The role of polymer architecture.BIOCHEMICAL ENGINEERING JOURNAL,128(DEC),218-227.
MLA Zhang, Su-Ling,et al."A novel polymer-grafted cation exchanger for high-capacity protein chromatography: The role of polymer architecture".BIOCHEMICAL ENGINEERING JOURNAL 128.DEC(2017):218-227.
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