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Rational design of Pleurotus eryngii versatile ligninolytic peroxidase for enhanced pH and thermal stability through structure-based protein engineering
Gao, Yu1; Li, Jian-Jun2; Zheng, Lanyan1; Du, Yuguang2
2017-11-01
Source PublicationPROTEIN ENGINEERING DESIGN & SELECTION
ISSN1741-0126
Volume30Issue:11Pages:743-751
Abstract

Versatile peroxidase (VP) from Pleurotus eryngii is a high redox potential peroxidase. It has aroused great biotechnological interest due to its ability to oxidize a wide range of substrates, but its application is still limited due to low pH and thermal stability. Since CiP (Coprinopsis cinerea peroxidase) and PNP (peanut peroxidase) exhibited higher pH and thermal stability than VP, several motifs, which might contribute to their pH and thermal stability, were identified through structure and sequence alignment. Six VP variants incorporating the beneficial motifs were designed and constructed. Most variants were nearly completely inactivated except V1 (Variant 1) and V4. V1 showed comparable activity to WT VP against ABTS, while V4 exhibited reduced activity. V1 displayed improved pH stability than WT VP, at pH 3.0 in particular, whereas the pH stability of V4 did not change a lot. The thermal stabilities of V1 and V4 were enhanced with T50 raised by 3 degrees C. The results demonstrated that variants containing the beneficial motifs of CiP and PNP conferred VP with improved pH and thermal stability.

Indexed BySCI
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/23509
Collection研究所(批量导入)
Corresponding AuthorLi, Jian-Jun; Zheng, Lanyan
Affiliation1.China Med Univ, Dept Microbiol & Parasitol, 77 Puhe Rd, Shenyang 110122, Liaoning, Peoples R China
2.Chinese Acad Sci, Key Lab Biopharmaceut Prod & Formulat Engn, Natl Key Lab Biochem Engn,PLA, Natl Engn Res Ctr Biotechnol Beijing,Inst Proc En, 1 North 2nd St, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Gao, Yu,Li, Jian-Jun,Zheng, Lanyan,et al. Rational design of Pleurotus eryngii versatile ligninolytic peroxidase for enhanced pH and thermal stability through structure-based protein engineering[J]. PROTEIN ENGINEERING DESIGN & SELECTION,2017,30(11):743-751.
APA Gao, Yu,Li, Jian-Jun,Zheng, Lanyan,&Du, Yuguang.(2017).Rational design of Pleurotus eryngii versatile ligninolytic peroxidase for enhanced pH and thermal stability through structure-based protein engineering.PROTEIN ENGINEERING DESIGN & SELECTION,30(11),743-751.
MLA Gao, Yu,et al."Rational design of Pleurotus eryngii versatile ligninolytic peroxidase for enhanced pH and thermal stability through structure-based protein engineering".PROTEIN ENGINEERING DESIGN & SELECTION 30.11(2017):743-751.
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