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Precise control of agarose media pore structure by regulating cooling rate
Chen, Chao1; Li, Xiunan1; Zhao, Dawei1; Li, Yaqiong2; Shi, Hong1; Ma, Guanghui1; Su, Zhiguo1,3
2017-11-01
发表期刊JOURNAL OF SEPARATION SCIENCE
ISSN1615-9306
卷号40期号:22页码:4467-4474
摘要

A porous structure is the key factor to successful chromatography separation. Agarose gel as one of the most popular porous media has been extensively used in chromatography separation. As the cooling process in the agarose gelation procedure can directly influence the pore structure, ten kinds of 4% agarosemedia with different cooling rates from 0.132 to 16.7 degrees C/min were synthesized, and the pore structure was determined accurately by using low-field NMR spectroscopy. The curves of pore structure and cooling rate can be divided into two stages with the boundary of 6 degrees C/min. In stage I, the pore structure met a power equation with the decrease of the cooling rate, and in stage II, the process reached a plateau. Confirmatory experiments proved that, by adjusting the cooling rate, a precise control of the pore structure of agarose media can be realized, furthermore, cooling rate optimization was an effective way to control the pore size of agarose media and can further tailor the pore structure for more effective separation of different proteins.

关键词Cooling Rate Low-field Nmr Spectroscopy Pore Size Pore Size Uniformity
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
DOI10.1002/jssc.201700546
收录类别SCI
语种英语
关键词[WOS]Size-exclusion Chromatography ; Stationary Phases ; Ionic-strength ; Gel Structure ; Transition ; Gradient ; Porosity ; Network
WOS研究方向Chemistry
WOS类目Chemistry, Analytical
项目资助者National Natural Science Foundation of China(21376248 ; National Key Scientific Instrument and Equipment Development Project(2013YQ14040502) ; National Basic Research Program(2013CB733604) ; 21506228)
WOS记录号WOS:000416228000019
引用统计
文献类型期刊论文
条目标识符http://ir.ipe.ac.cn/handle/122111/23514
专题研究所(批量导入)
通讯作者Li, Xiunan; Su, Zhiguo
作者单位1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing, Peoples R China
2.Sichuan Univ, Key Lab Drug Targeting & Drug Delivery Syst, Minist Educ, West China Sch Pharm, Chengdu, Sichuan, Peoples R China
3.Jiangsu Natl Synerget Innovat Ctr Adv Mat SICAM, Nanjing, Jiangsu, Peoples R China
推荐引用方式
GB/T 7714
Chen, Chao,Li, Xiunan,Zhao, Dawei,et al. Precise control of agarose media pore structure by regulating cooling rate[J]. JOURNAL OF SEPARATION SCIENCE,2017,40(22):4467-4474.
APA Chen, Chao.,Li, Xiunan.,Zhao, Dawei.,Li, Yaqiong.,Shi, Hong.,...&Su, Zhiguo.(2017).Precise control of agarose media pore structure by regulating cooling rate.JOURNAL OF SEPARATION SCIENCE,40(22),4467-4474.
MLA Chen, Chao,et al."Precise control of agarose media pore structure by regulating cooling rate".JOURNAL OF SEPARATION SCIENCE 40.22(2017):4467-4474.
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