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Precise control of agarose media pore structure by regulating cooling rate
Chen, Chao1; Li, Xiunan1; Zhao, Dawei1; Li, Yaqiong2; Shi, Hong1; Ma, Guanghui1; Su, Zhiguo1,3

A porous structure is the key factor to successful chromatography separation. Agarose gel as one of the most popular porous media has been extensively used in chromatography separation. As the cooling process in the agarose gelation procedure can directly influence the pore structure, ten kinds of 4% agarosemedia with different cooling rates from 0.132 to 16.7 degrees C/min were synthesized, and the pore structure was determined accurately by using low-field NMR spectroscopy. The curves of pore structure and cooling rate can be divided into two stages with the boundary of 6 degrees C/min. In stage I, the pore structure met a power equation with the decrease of the cooling rate, and in stage II, the process reached a plateau. Confirmatory experiments proved that, by adjusting the cooling rate, a precise control of the pore structure of agarose media can be realized, furthermore, cooling rate optimization was an effective way to control the pore size of agarose media and can further tailor the pore structure for more effective separation of different proteins.

KeywordCooling Rate Low-field Nmr Spectroscopy Pore Size Pore Size Uniformity
WOS HeadingsScience & Technology ; Physical Sciences
Indexed BySCI
WOS KeywordSize-exclusion Chromatography ; Stationary Phases ; Ionic-strength ; Gel Structure ; Transition ; Gradient ; Porosity ; Network
WOS Research AreaChemistry
WOS SubjectChemistry, Analytical
Funding OrganizationNational Natural Science Foundation of China(21376248 ; National Key Scientific Instrument and Equipment Development Project(2013YQ14040502) ; National Basic Research Program(2013CB733604) ; 21506228)
WOS IDWOS:000416228000019
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Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Corresponding AuthorLi, Xiunan; Su, Zhiguo
Affiliation1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing, Peoples R China
2.Sichuan Univ, Key Lab Drug Targeting & Drug Delivery Syst, Minist Educ, West China Sch Pharm, Chengdu, Sichuan, Peoples R China
3.Jiangsu Natl Synerget Innovat Ctr Adv Mat SICAM, Nanjing, Jiangsu, Peoples R China
Recommended Citation
GB/T 7714
Chen, Chao,Li, Xiunan,Zhao, Dawei,et al. Precise control of agarose media pore structure by regulating cooling rate[J]. JOURNAL OF SEPARATION SCIENCE,2017,40(22):4467-4474.
APA Chen, Chao.,Li, Xiunan.,Zhao, Dawei.,Li, Yaqiong.,Shi, Hong.,...&Su, Zhiguo.(2017).Precise control of agarose media pore structure by regulating cooling rate.JOURNAL OF SEPARATION SCIENCE,40(22),4467-4474.
MLA Chen, Chao,et al."Precise control of agarose media pore structure by regulating cooling rate".JOURNAL OF SEPARATION SCIENCE 40.22(2017):4467-4474.
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