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甘油介导的疏水色谱复性重组人α-干扰素
李京京; 王芳薇; 刘永东; 罗天乐; 闭静秀; 苏志国
2004
Conference Name第一届全国化学工程与生物化工年会
Source Publication第一届全国化学工程与生物化工年会论文摘要集(下)
Pages1
Conference Date2004-11
Conference Place中国
Abstract<正> 蛋白质的折叠复性是生物工程下游技术的瓶颈问题,传统的复性方法由于聚集沉淀而活性蛋白收率很低。采用色谱方法复性蛋白质具有独特的优点,近年来倍受蛋白质工作者的关注。凝胶过滤色谱、离子交换色谱和金属螯和色谱都曾被成功地用于复性某些蛋白质。本实验室采用发展了脲梯度以及脲-pH值双梯度的色谱复性方法,使复性收率
Keyword疏水色谱:4857 人α-干扰素:4342 折叠复性:3473 蛋白质:3318 凝胶过滤色谱:2846 离子交换色谱:2727 聚集沉淀:2331 下游技术:2292 色谱方法:2070 活性蛋白:1986
Language中文
Document Type会议论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/2395
Collection研究所(批量导入)
Recommended Citation
GB/T 7714
李京京,王芳薇,刘永东,等. 甘油介导的疏水色谱复性重组人α-干扰素[C],2004:1.
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