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ENHANCEMENT OF PHENYLALANINE AMMONIA LYASE PRODUCTION FROM RHODOTORULA MUCILAGINOSA BY OPTIMIZATION OF CULTURE CONDITIONS IN BATCH AND FED-BATCH
Zhang, Si1; Cui, Jian-Dong1,2,3
2012-12-01
Source PublicationBIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT
ISSN1310-2818
Volume26Issue:6Pages:3418-3423
AbstractThe fermentation of phenylalanine ammonia lyase (PAL) by Rhodotorula mucilaginosa (R. mucilaginosa) was optimized PAL production was enhanced 8.0-fold from an initial activity of 5 U/g (DCW) with the incorporation of optimization of medium composition, controlled-pH operation, the strategy of batch supplementation with substrate induction and other culture conditions, and the implementation of a fed-batch process. A significant influence of peptone and inoculum size on PAL production was evaluated by Plackett-Burman design and the path of steepest ascent experiment. Among the various amino acids used, it was found that L-Phenylalanine could efficiently induce the biosynthesis of PAL. PAL activity was increased by 18.5 % in comparison with the control. Glucose concentration significantly affected PAL synthesis. When the glucose concentration was I g/L, PAL activity was enhanced by 1.82- and 6-fold in comparison with those at 5 g/L and 10 g/L, respectively To further increase PAL production, controlled-pH operation and the strategy of batch supplementation with the inducer was performed in a 5 L stirred tank reactor The maximum production of PAL reached 41 U/g (DCW). Biotechnol. & Biotechnol. Eq. 2012, 26(6), 3418-3423
KeywordEnzyme Activity Phenylalanine Ammonia Lyase Batch And fed-Batch Processing Optimization Rhodotorula Mucilaginosa
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.5504/BBEQ.2012.0106
Indexed BySCI
Language英语
WOS KeywordRECOMBINANT ESCHERICHIA-COLI ; UNCONTROLLED-PH OPERATIONS ; RHODOSPORIDIUM-TORULOIDES ; GLUTINIS ; STABILIZATION ; EXPRESSION ; INDUCTION ; NITROGEN ; YEAST ; CELLS
WOS Research AreaBiotechnology & Applied Microbiology
WOS SubjectBiotechnology & Applied Microbiology
Funding OrganizationNational Natural Science Foundation of China (NSFC)(21072041) ; National Key Laboratory of Biochemical Engineering(KF2010-12) ; Foundation of Tianjin Key Laboratory of Industrial Microbiology (Tianjin University of Science and Technology), P.R. China(GYWSW02) ; Foundation of Hebei University of Science and Technology for Distinguished Young Scientists
WOS IDWOS:000313856200016
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/23970
Collection生化工程国家重点实验室
Affiliation1.Hebei Univ Sci & Technol, Key Lab Pharmaceut Mol Chem, Shijiazhuang, Peoples R China
2.Chinese Acad Sci, Natl Key Lab Biochem Engn, Beijing, Peoples R China
3.Tianjin Univ Sci & Technol, Key Lab Ind Microbiol, Tianjin, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Si,Cui, Jian-Dong. ENHANCEMENT OF PHENYLALANINE AMMONIA LYASE PRODUCTION FROM RHODOTORULA MUCILAGINOSA BY OPTIMIZATION OF CULTURE CONDITIONS IN BATCH AND FED-BATCH[J]. BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT,2012,26(6):3418-3423.
APA Zhang, Si,&Cui, Jian-Dong.(2012).ENHANCEMENT OF PHENYLALANINE AMMONIA LYASE PRODUCTION FROM RHODOTORULA MUCILAGINOSA BY OPTIMIZATION OF CULTURE CONDITIONS IN BATCH AND FED-BATCH.BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT,26(6),3418-3423.
MLA Zhang, Si,et al."ENHANCEMENT OF PHENYLALANINE AMMONIA LYASE PRODUCTION FROM RHODOTORULA MUCILAGINOSA BY OPTIMIZATION OF CULTURE CONDITIONS IN BATCH AND FED-BATCH".BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT 26.6(2012):3418-3423.
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