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A multiplex microfluidic loop-mediated isothermal amplification array for detection of malaria-related parasites and vectors
Mao, Rui1,2,3; Ge, Ge1,2; Wang, Zhuo1,2; Hao, Rongzhang5; Zhang, Guohao1,2; Yang, Zhenzhou1,2; Lin, Bincheng1,2; Ma, Yajun4; Liu, Hongtao1,2; Du, Yuguang1,2
2018-02-01
发表期刊ACTA TROPICA
ISSN0001-706X
卷号178页码:86-92
摘要Malaria infection poses a great threaten to public health even nowadays. The conventional diagnosis tools of malaria parasites and vectors require systematic training for the observers accompanied by the low throughput. In this study, a new detection system, i.e., multiplex microfluidic loop-mediated isothermal amplification (m mu LAMP) array, was developed to provide a convenient, rapid and economical detection system for malaria diagnosis. A microfluidic-based detection chip was designed and developed, targeting the conserved gene of four Anopheles and two Plasmodium species responsible for most of the malaria cases occurred in China. The DNA preparation of Anopheles and Plasmodium samples was realized by using a newly-developed DNA extraction method. For this m mu LAMP array system, the detection limit was determined to be 1 pg of targeting DNA with high sensitivity (>95%) and specificity (100%). Further, the accuracy of such m mu LAMP analysis was evaluated by the analysis of 48 Anopheles mosquito samples, of which 30 were termed to be target Anopheles, displaying high consistency with that by morphological analysis. In conclusion, the m mu LAMP detection system was proved to be a visible, sensitive, specific and high-throughput diagnostic tool. Considering the portable manipulation of such detection system, our studies shed light on its potential application of malaria surveillance on the spot.
关键词Anopheles Mosquito Plasmodium Loop-mediated Isothermal Amplification (Lamp) Multiplex Microfluidic Chip Morphological Analysis
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine
DOI10.1016/j.actatropica.2017.10.025
收录类别SCI
语种英语
关键词[WOS]RAPID IDENTIFICATION ; LAMP ; DIAGNOSIS ; MOSQUITOS ; MICROSCOPY ; DEVICES
WOS研究方向Parasitology ; Tropical Medicine
WOS类目Parasitology ; Tropical Medicine
项目资助者Chinese academy of sciences(CXJJ-15Z012) ; National High Technology Research and Development Program of China(2015AA020929) ; Beijing Nova Program(Z141107001814071)
WOS记录号WOS:000423644300013
引用统计
文献类型期刊论文
条目标识符http://ir.ipe.ac.cn/handle/122111/23987
专题生化工程国家重点实验室
作者单位1.Chinese Acad Sci, Key Lab Biopharmaceut Prod & Formulat Engn, PLA, Beijing 100190, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Second Mil Med Univ, Dept Pathogen Biol, Shanghai 200433, Peoples R China
5.Acad Mil Med Sci, Inst Dis Control & Prevent, Beijing 100071, Peoples R China
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Mao, Rui,Ge, Ge,Wang, Zhuo,et al. A multiplex microfluidic loop-mediated isothermal amplification array for detection of malaria-related parasites and vectors[J]. ACTA TROPICA,2018,178:86-92.
APA Mao, Rui.,Ge, Ge.,Wang, Zhuo.,Hao, Rongzhang.,Zhang, Guohao.,...&Du, Yuguang.(2018).A multiplex microfluidic loop-mediated isothermal amplification array for detection of malaria-related parasites and vectors.ACTA TROPICA,178,86-92.
MLA Mao, Rui,et al."A multiplex microfluidic loop-mediated isothermal amplification array for detection of malaria-related parasites and vectors".ACTA TROPICA 178(2018):86-92.
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