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A multiplex microfluidic loop-mediated isothermal amplification array for detection of malaria-related parasites and vectors
Mao, Rui1,2,3; Ge, Ge1,2; Wang, Zhuo1,2; Hao, Rongzhang5; Zhang, Guohao1,2; Yang, Zhenzhou1,2; Lin, Bincheng1,2; Ma, Yajun4; Liu, Hongtao1,2; Du, Yuguang1,2
2018-02-01
Source PublicationACTA TROPICA
ISSN0001-706X
Volume178Pages:86-92
AbstractMalaria infection poses a great threaten to public health even nowadays. The conventional diagnosis tools of malaria parasites and vectors require systematic training for the observers accompanied by the low throughput. In this study, a new detection system, i.e., multiplex microfluidic loop-mediated isothermal amplification (m mu LAMP) array, was developed to provide a convenient, rapid and economical detection system for malaria diagnosis. A microfluidic-based detection chip was designed and developed, targeting the conserved gene of four Anopheles and two Plasmodium species responsible for most of the malaria cases occurred in China. The DNA preparation of Anopheles and Plasmodium samples was realized by using a newly-developed DNA extraction method. For this m mu LAMP array system, the detection limit was determined to be 1 pg of targeting DNA with high sensitivity (>95%) and specificity (100%). Further, the accuracy of such m mu LAMP analysis was evaluated by the analysis of 48 Anopheles mosquito samples, of which 30 were termed to be target Anopheles, displaying high consistency with that by morphological analysis. In conclusion, the m mu LAMP detection system was proved to be a visible, sensitive, specific and high-throughput diagnostic tool. Considering the portable manipulation of such detection system, our studies shed light on its potential application of malaria surveillance on the spot.
KeywordAnopheles Mosquito Plasmodium Loop-mediated Isothermal Amplification (Lamp) Multiplex Microfluidic Chip Morphological Analysis
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1016/j.actatropica.2017.10.025
Indexed BySCI
Language英语
WOS KeywordRAPID IDENTIFICATION ; LAMP ; DIAGNOSIS ; MOSQUITOS ; MICROSCOPY ; DEVICES
WOS Research AreaParasitology ; Tropical Medicine
WOS SubjectParasitology ; Tropical Medicine
Funding OrganizationChinese academy of sciences(CXJJ-15Z012) ; National High Technology Research and Development Program of China(2015AA020929) ; Beijing Nova Program(Z141107001814071)
WOS IDWOS:000423644300013
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/23987
Collection生化工程国家重点实验室
Affiliation1.Chinese Acad Sci, Key Lab Biopharmaceut Prod & Formulat Engn, PLA, Beijing 100190, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Second Mil Med Univ, Dept Pathogen Biol, Shanghai 200433, Peoples R China
5.Acad Mil Med Sci, Inst Dis Control & Prevent, Beijing 100071, Peoples R China
Recommended Citation
GB/T 7714
Mao, Rui,Ge, Ge,Wang, Zhuo,et al. A multiplex microfluidic loop-mediated isothermal amplification array for detection of malaria-related parasites and vectors[J]. ACTA TROPICA,2018,178:86-92.
APA Mao, Rui.,Ge, Ge.,Wang, Zhuo.,Hao, Rongzhang.,Zhang, Guohao.,...&Du, Yuguang.(2018).A multiplex microfluidic loop-mediated isothermal amplification array for detection of malaria-related parasites and vectors.ACTA TROPICA,178,86-92.
MLA Mao, Rui,et al."A multiplex microfluidic loop-mediated isothermal amplification array for detection of malaria-related parasites and vectors".ACTA TROPICA 178(2018):86-92.
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