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Role of oxygen supply in alpha, omega-dodecanedioic acid biosynthesis from n-dodecane by Candida viswanathii ipe-1: Effect of stirring speed and aeration
Cao, Weifeng1; Wang, Yujue1,2; Luo, Jianquan1,2; Yin, Junxiang3; Wan, Yinhua1,2
2018-03-01
发表期刊ENGINEERING IN LIFE SCIENCES
ISSN1618-0240
卷号18期号:3页码:196-203
摘要alpha, omega-Dodecanedioic acid (DC12) usually serves as a monomer of polyamides or some special nylons. During the biosynthesis, oxygenation cascaded in conversion of hydrophobic n-dodecane to DC12, while the oxidation of n-dodecane took place in the intracellular space. Therefore, it was important to investigate the role of oxygen supply on the cell growth and DC12 biosynthesis. It was found that stirring speed and aeration influenced the dissolved oxygen (DO) concentration which in turn affected cell growth as well as DC12 biosynthesis. However, the effect of culture redox potential (Orp) level on DC12 biosynthesis was more significant than that of DO level. For DC12 biosynthesis, the first step was to form the emulsion droplets through the interaction of n-dodecane and the cell. When the stirring speed was enhanced, slits in the surface layer of the emulsion droplets would be increased. Thus, the substances transportation by water through the slits would be intensified, leading to an enhanced DC12 production. Compared with the batch culture at a lower stirring speed (400 rpm) without culture redox potential (Orp) control, the DC12 concentration was increased by 5 times up to 201.3 g/L with Orp controlled above 0 mV at a higher stirring speed (800 rpm).
关键词Candida Viswanathii Alpha N-dodecane Omega-dicarboxylic Acids Alpha Redox Potential Omega-dodecanedioic Acid
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine
DOI10.1002/elsc.201700142
收录类别SCI
语种英语
关键词[WOS]ALPHA,OMEGA-DODECANEDIOIC ACID ; FERMENTATIVE PRODUCTION ; YARROWIA-LIPOLYTICA ; DICARBOXYLIC-ACID ; ESCHERICHIA-COLI ; CONTROL STRATEGY ; TROPICALIS ; AGITATION ; PRODUCTIVITY ; SUBSTRATE
WOS研究方向Biotechnology & Applied Microbiology
WOS类目Biotechnology & Applied Microbiology
项目资助者National High Technology Research and Development Program of China(2015AA021002 ; National Natural Science Foundation of China(21406240) ; 2014AA021005)
WOS记录号WOS:000426610700005
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ipe.ac.cn/handle/122111/24010
专题生化工程国家重点实验室
作者单位1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Chinese Acad Sci, Univ Chinese Acad Sci, Beijing, Peoples R China
3.China Natl Ctr Biotechnol Dev, Beijing, Peoples R China
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Cao, Weifeng,Wang, Yujue,Luo, Jianquan,et al. Role of oxygen supply in alpha, omega-dodecanedioic acid biosynthesis from n-dodecane by Candida viswanathii ipe-1: Effect of stirring speed and aeration[J]. ENGINEERING IN LIFE SCIENCES,2018,18(3):196-203.
APA Cao, Weifeng,Wang, Yujue,Luo, Jianquan,Yin, Junxiang,&Wan, Yinhua.(2018).Role of oxygen supply in alpha, omega-dodecanedioic acid biosynthesis from n-dodecane by Candida viswanathii ipe-1: Effect of stirring speed and aeration.ENGINEERING IN LIFE SCIENCES,18(3),196-203.
MLA Cao, Weifeng,et al."Role of oxygen supply in alpha, omega-dodecanedioic acid biosynthesis from n-dodecane by Candida viswanathii ipe-1: Effect of stirring speed and aeration".ENGINEERING IN LIFE SCIENCES 18.3(2018):196-203.
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