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Role of oxygen supply in alpha, omega-dodecanedioic acid biosynthesis from n-dodecane by Candida viswanathii ipe-1: Effect of stirring speed and aeration
Cao, Weifeng1; Wang, Yujue1,2; Luo, Jianquan1,2; Yin, Junxiang3; Wan, Yinhua1,2
2018-03-01
Source PublicationENGINEERING IN LIFE SCIENCES
ISSN1618-0240
Volume18Issue:3Pages:196-203
Abstractalpha, omega-Dodecanedioic acid (DC12) usually serves as a monomer of polyamides or some special nylons. During the biosynthesis, oxygenation cascaded in conversion of hydrophobic n-dodecane to DC12, while the oxidation of n-dodecane took place in the intracellular space. Therefore, it was important to investigate the role of oxygen supply on the cell growth and DC12 biosynthesis. It was found that stirring speed and aeration influenced the dissolved oxygen (DO) concentration which in turn affected cell growth as well as DC12 biosynthesis. However, the effect of culture redox potential (Orp) level on DC12 biosynthesis was more significant than that of DO level. For DC12 biosynthesis, the first step was to form the emulsion droplets through the interaction of n-dodecane and the cell. When the stirring speed was enhanced, slits in the surface layer of the emulsion droplets would be increased. Thus, the substances transportation by water through the slits would be intensified, leading to an enhanced DC12 production. Compared with the batch culture at a lower stirring speed (400 rpm) without culture redox potential (Orp) control, the DC12 concentration was increased by 5 times up to 201.3 g/L with Orp controlled above 0 mV at a higher stirring speed (800 rpm).
KeywordCandida Viswanathii Alpha N-dodecane Omega-dicarboxylic Acids Alpha Redox Potential Omega-dodecanedioic Acid
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1002/elsc.201700142
Indexed BySCI
Language英语
WOS KeywordALPHA,OMEGA-DODECANEDIOIC ACID ; FERMENTATIVE PRODUCTION ; YARROWIA-LIPOLYTICA ; DICARBOXYLIC-ACID ; ESCHERICHIA-COLI ; CONTROL STRATEGY ; TROPICALIS ; AGITATION ; PRODUCTIVITY ; SUBSTRATE
WOS Research AreaBiotechnology & Applied Microbiology
WOS SubjectBiotechnology & Applied Microbiology
Funding OrganizationNational High Technology Research and Development Program of China(2015AA021002 ; National Natural Science Foundation of China(21406240) ; 2014AA021005)
WOS IDWOS:000426610700005
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/24010
Collection生化工程国家重点实验室
Affiliation1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Chinese Acad Sci, Univ Chinese Acad Sci, Beijing, Peoples R China
3.China Natl Ctr Biotechnol Dev, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Cao, Weifeng,Wang, Yujue,Luo, Jianquan,et al. Role of oxygen supply in alpha, omega-dodecanedioic acid biosynthesis from n-dodecane by Candida viswanathii ipe-1: Effect of stirring speed and aeration[J]. ENGINEERING IN LIFE SCIENCES,2018,18(3):196-203.
APA Cao, Weifeng,Wang, Yujue,Luo, Jianquan,Yin, Junxiang,&Wan, Yinhua.(2018).Role of oxygen supply in alpha, omega-dodecanedioic acid biosynthesis from n-dodecane by Candida viswanathii ipe-1: Effect of stirring speed and aeration.ENGINEERING IN LIFE SCIENCES,18(3),196-203.
MLA Cao, Weifeng,et al."Role of oxygen supply in alpha, omega-dodecanedioic acid biosynthesis from n-dodecane by Candida viswanathii ipe-1: Effect of stirring speed and aeration".ENGINEERING IN LIFE SCIENCES 18.3(2018):196-203.
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