| Development of a Novel Micro-Aerobic Cultivation Strategy for High Potential CotA Laccase Production | |
| Samak, Nadia A.1,2,3; Hu, Jianhua1,2,3; Wang, Kefeng1,2,3; Guo, Chen1,2; Liu, Chunzhao1,2 | |
| 2018-03-01 | |
| Source Publication | WASTE AND BIOMASS VALORIZATION
 |
| ISSN | 1877-2641 |
| Volume | 9Issue:3Pages:369-377 |
| Abstract | Recently, bacterial laccases has drawn researchers' interest due to their ability to overcome high pH and salt concentration conditions compared to fungal laccases. Here we report a novel micro-aerobic cultivation strategy for enhancing CotA laccase expression and study its application for dye decolorization. Micro-aerobic cultivation of Escherichia coli BL21 (DE3) strain carrying pT7-FLAG-MAT-TAG-1-CotA had significantly enhanced CotA laccase activity up to 13903 U/L. The most unique findings of this investigation are that micro-aerobic cultivation strategy enhanced the reactive oxygen species production which consequently led to the over expression of CotA laccase gene. Malachite green, Crystal violet, Congo red and Bromophenol blue were efficiently decolorized by using purified CotA laccase without presence of any mediators at pH 6 and 9. These results provide a great platform for the dynamic production and application of bacterial laccase in industry. |
| Keyword | Bacillus Subtilis Bacterial Laccase Cota Iptg Micro-aerobic Condition Ros |
| Subtype | Article |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| DOI | 10.1007/s12649-016-9824-6 |
| Indexed By | SCI |
| Language | 英语 |
| WOS Keyword | RECOMBINANT ESCHERICHIA-COLI ; GENE-EXPRESSION ; BACILLUS-LICHENIFORMIS ; OXIDATIVE STRESS ; WASTE-WATER ; DECOLORIZATION ; CLONING ; DYES ; PROTEINS ; ACCUMULATION |
| WOS Research Area | Environmental Sciences & Ecology |
| WOS Subject | Environmental Sciences |
| Funding Organization | National Key Technology Research and Development Program of China(2015BAK45B01) ; National Natural Science Foundation of China(21476242) ; National Basic Research Program (973 Program) of China(2013CB733600) ; Chinese Government |
| WOS ID | WOS:000425332300004 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ipe.ac.cn/handle/122111/24027 |
| Collection | 生化工程国家重点实验室 |
| Affiliation | 1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, Key Lab Green Proc & Engn, Beijing 100190, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| Recommended Citation GB/T 7714 | Samak, Nadia A.,Hu, Jianhua,Wang, Kefeng,et al. Development of a Novel Micro-Aerobic Cultivation Strategy for High Potential CotA Laccase Production[J]. WASTE AND BIOMASS VALORIZATION,2018,9(3):369-377. |
| APA | Samak, Nadia A.,Hu, Jianhua,Wang, Kefeng,Guo, Chen,&Liu, Chunzhao.(2018).Development of a Novel Micro-Aerobic Cultivation Strategy for High Potential CotA Laccase Production.WASTE AND BIOMASS VALORIZATION,9(3),369-377. |
| MLA | Samak, Nadia A.,et al."Development of a Novel Micro-Aerobic Cultivation Strategy for High Potential CotA Laccase Production".WASTE AND BIOMASS VALORIZATION 9.3(2018):369-377. |
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