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Development of a Novel Micro-Aerobic Cultivation Strategy for High Potential CotA Laccase Production
Samak, Nadia A.1,2,3; Hu, Jianhua1,2,3; Wang, Kefeng1,2,3; Guo, Chen1,2; Liu, Chunzhao1,2
2018-03-01
Source PublicationWASTE AND BIOMASS VALORIZATION
ISSN1877-2641
Volume9Issue:3Pages:369-377
AbstractRecently, bacterial laccases has drawn researchers' interest due to their ability to overcome high pH and salt concentration conditions compared to fungal laccases. Here we report a novel micro-aerobic cultivation strategy for enhancing CotA laccase expression and study its application for dye decolorization. Micro-aerobic cultivation of Escherichia coli BL21 (DE3) strain carrying pT7-FLAG-MAT-TAG-1-CotA had significantly enhanced CotA laccase activity up to 13903 U/L. The most unique findings of this investigation are that micro-aerobic cultivation strategy enhanced the reactive oxygen species production which consequently led to the over expression of CotA laccase gene. Malachite green, Crystal violet, Congo red and Bromophenol blue were efficiently decolorized by using purified CotA laccase without presence of any mediators at pH 6 and 9. These results provide a great platform for the dynamic production and application of bacterial laccase in industry.
KeywordBacillus Subtilis Bacterial Laccase Cota Iptg Micro-aerobic Condition Ros
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1007/s12649-016-9824-6
Indexed BySCI
Language英语
WOS KeywordRECOMBINANT ESCHERICHIA-COLI ; GENE-EXPRESSION ; BACILLUS-LICHENIFORMIS ; OXIDATIVE STRESS ; WASTE-WATER ; DECOLORIZATION ; CLONING ; DYES ; PROTEINS ; ACCUMULATION
WOS Research AreaEnvironmental Sciences & Ecology
WOS SubjectEnvironmental Sciences
Funding OrganizationNational Key Technology Research and Development Program of China(2015BAK45B01) ; National Natural Science Foundation of China(21476242) ; National Basic Research Program (973 Program) of China(2013CB733600) ; Chinese Government
WOS IDWOS:000425332300004
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/24027
Collection生化工程国家重点实验室
Affiliation1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, Key Lab Green Proc & Engn, Beijing 100190, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Samak, Nadia A.,Hu, Jianhua,Wang, Kefeng,et al. Development of a Novel Micro-Aerobic Cultivation Strategy for High Potential CotA Laccase Production[J]. WASTE AND BIOMASS VALORIZATION,2018,9(3):369-377.
APA Samak, Nadia A.,Hu, Jianhua,Wang, Kefeng,Guo, Chen,&Liu, Chunzhao.(2018).Development of a Novel Micro-Aerobic Cultivation Strategy for High Potential CotA Laccase Production.WASTE AND BIOMASS VALORIZATION,9(3),369-377.
MLA Samak, Nadia A.,et al."Development of a Novel Micro-Aerobic Cultivation Strategy for High Potential CotA Laccase Production".WASTE AND BIOMASS VALORIZATION 9.3(2018):369-377.
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