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CotA laccase immobilized on functionalized magnetic graphene oxide nano-sheets for efficient biocatalysis
Samak, Nadia A.1,2,4; Tan, Yeqiang3; Sui, Kunyan3; Xia, Ting-Ting1,2,4; Wang, Kefeng1,2,4; Guo, Chen1,2; Liu, Chunzhao1,2,3
2018-02-01
Source PublicationMOLECULAR CATALYSIS
ISSN2468-8231
Volume445Pages:269-278
AbstractIn this work, a novel type of functionalized magnetic graphene oxide (MGO) nano-sheets which have the ability to capture His-tagged CotA laccase was synthesized. N alpha,N alpha-Bis(carboxymethy1)-L-lysine hydrate (NTA-NH2) was attached on MGO then chelated with Ni2+ and Cu2+ separately. The Cu2+-chelated MGO (MGO-NTA-Cu2+) exhibited the highest adsorption capacity of 177 mg/g-support when compared with both MGO and Ni2+-chelated MGO (MGO-NTA-Ni2+) which exhibited an adsorption capacity of 49.9 and 145 mg/g-support, respectively. CotA laccase immobilized on MGO-NTA-Cu2+ nano-sheets showed the highest activity recovery when compared with that immobilized on MGO-NTA-Ni2+ nano-sheets. The catalytic properties of MGO-NTA-Cu-CotA laccase were significantly improved in comparison with those of the free laccase. MGO-NTA-Cu-CotA laccase showed efficient decolorization for Congo Red (CR), and the decolorization rate reached 100% after 5 h reaction at 60 degrees C and pH 8. Furthermore, MGO-NTA-Cu-CotA laccase retained 89.4% of its initial activity after 10 consecutive cycles. Together these results indicated that the Cu2+ chelated MGO nano-sheets are promising support for efficient CotA laccase immobilization for environmental and industrial applications under high temperature and alkaline condition. (C) 2017 Elsevier B.V. All rights reserved.
KeywordImmobilization Magnetic Graphene Oxide Mgo-nta-cu2++ Mgo-nta-ni2++ Congo Red
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
DOI10.1016/j.mcat.2017.12.004
Indexed BySCI
Language英语
WOS KeywordCHELATE AFFINITY INTERACTION ; TRAMETES-VERSICOLOR LACCASE ; AQUEOUS-SOLUTION ; ENZYME-ACTIVITY ; DYE ; NANOPARTICLES ; REMOVAL ; ADSORPTION ; STABILITY ; ACID
WOS Research AreaChemistry
WOS SubjectChemistry, Physical
Funding OrganizationNational Natural Science Foundation of China(21476242) ; National Key Technology Research and Development Program of China(2015BAK45B01) ; National Basic Research Program (973 Program) of China(2013CB733600) ; Chinese Government
WOS IDWOS:000424724500031
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/24064
Collection生化工程国家重点实验室
Affiliation1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, Key Lab Green Proc & Engn, Beijing 100190, Peoples R China
3.Qingdao Univ, Sch Mat Sci & Engn, Collaborat Innovat Ctr Marine Biomass Fibers Mat, Qingdao 266071, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Samak, Nadia A.,Tan, Yeqiang,Sui, Kunyan,et al. CotA laccase immobilized on functionalized magnetic graphene oxide nano-sheets for efficient biocatalysis[J]. MOLECULAR CATALYSIS,2018,445:269-278.
APA Samak, Nadia A..,Tan, Yeqiang.,Sui, Kunyan.,Xia, Ting-Ting.,Wang, Kefeng.,...&Liu, Chunzhao.(2018).CotA laccase immobilized on functionalized magnetic graphene oxide nano-sheets for efficient biocatalysis.MOLECULAR CATALYSIS,445,269-278.
MLA Samak, Nadia A.,et al."CotA laccase immobilized on functionalized magnetic graphene oxide nano-sheets for efficient biocatalysis".MOLECULAR CATALYSIS 445(2018):269-278.
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