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CotA laccase immobilized on functionalized magnetic graphene oxide nano-sheets for efficient biocatalysis
Samak, Nadia A.1,2,4; Tan, Yeqiang3; Sui, Kunyan3; Xia, Ting-Ting1,2,4; Wang, Kefeng1,2,4; Guo, Chen1,2; Liu, Chunzhao1,2,3
2018-02-01
发表期刊MOLECULAR CATALYSIS
ISSN2468-8231
卷号445页码:269-278
摘要In this work, a novel type of functionalized magnetic graphene oxide (MGO) nano-sheets which have the ability to capture His-tagged CotA laccase was synthesized. N alpha,N alpha-Bis(carboxymethy1)-L-lysine hydrate (NTA-NH2) was attached on MGO then chelated with Ni2+ and Cu2+ separately. The Cu2+-chelated MGO (MGO-NTA-Cu2+) exhibited the highest adsorption capacity of 177 mg/g-support when compared with both MGO and Ni2+-chelated MGO (MGO-NTA-Ni2+) which exhibited an adsorption capacity of 49.9 and 145 mg/g-support, respectively. CotA laccase immobilized on MGO-NTA-Cu2+ nano-sheets showed the highest activity recovery when compared with that immobilized on MGO-NTA-Ni2+ nano-sheets. The catalytic properties of MGO-NTA-Cu-CotA laccase were significantly improved in comparison with those of the free laccase. MGO-NTA-Cu-CotA laccase showed efficient decolorization for Congo Red (CR), and the decolorization rate reached 100% after 5 h reaction at 60 degrees C and pH 8. Furthermore, MGO-NTA-Cu-CotA laccase retained 89.4% of its initial activity after 10 consecutive cycles. Together these results indicated that the Cu2+ chelated MGO nano-sheets are promising support for efficient CotA laccase immobilization for environmental and industrial applications under high temperature and alkaline condition. (C) 2017 Elsevier B.V. All rights reserved.
关键词Immobilization Magnetic Graphene Oxide Mgo-nta-cu2++ Mgo-nta-ni2++ Congo Red
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
DOI10.1016/j.mcat.2017.12.004
收录类别SCI
语种英语
关键词[WOS]CHELATE AFFINITY INTERACTION ; TRAMETES-VERSICOLOR LACCASE ; AQUEOUS-SOLUTION ; ENZYME-ACTIVITY ; DYE ; NANOPARTICLES ; REMOVAL ; ADSORPTION ; STABILITY ; ACID
WOS研究方向Chemistry
WOS类目Chemistry, Physical
项目资助者National Natural Science Foundation of China(21476242) ; National Key Technology Research and Development Program of China(2015BAK45B01) ; National Basic Research Program (973 Program) of China(2013CB733600) ; Chinese Government
WOS记录号WOS:000424724500031
引用统计
文献类型期刊论文
条目标识符http://ir.ipe.ac.cn/handle/122111/24064
专题生化工程国家重点实验室
作者单位1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, Key Lab Green Proc & Engn, Beijing 100190, Peoples R China
3.Qingdao Univ, Sch Mat Sci & Engn, Collaborat Innovat Ctr Marine Biomass Fibers Mat, Qingdao 266071, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
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Samak, Nadia A.,Tan, Yeqiang,Sui, Kunyan,et al. CotA laccase immobilized on functionalized magnetic graphene oxide nano-sheets for efficient biocatalysis[J]. MOLECULAR CATALYSIS,2018,445:269-278.
APA Samak, Nadia A..,Tan, Yeqiang.,Sui, Kunyan.,Xia, Ting-Ting.,Wang, Kefeng.,...&Liu, Chunzhao.(2018).CotA laccase immobilized on functionalized magnetic graphene oxide nano-sheets for efficient biocatalysis.MOLECULAR CATALYSIS,445,269-278.
MLA Samak, Nadia A.,et al."CotA laccase immobilized on functionalized magnetic graphene oxide nano-sheets for efficient biocatalysis".MOLECULAR CATALYSIS 445(2018):269-278.
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