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Alginate dialdehyde meets nylon membrane: a versatile platform for facile and green fabrication of membrane adsorbers
Khan, M. Kamran1,2; Luo, Jianquan1,2; Wang, Zhaoshuai1,2; Khan, Rashid1,2; Chen, Xiangrong1; Wan, Yinhua1,2
2018-03-21
发表期刊JOURNAL OF MATERIALS CHEMISTRY B
ISSN2050-750X
卷号6期号:11页码:1640-1649
摘要Alginate dialdehyde (ADA), a biocompatible polymer, was used as an intermediate layer on a nylon membrane to readily fabricate cation exchange (CEX), metal-affinity (Me-affinity), histidine-affinity (His-affinity) and peptide-affinity (Pep-affinity) membrane adsorbers without any organic solvent usage. All the membrane adsorbers exhibited a high selectivity in the fractionation of a IgG (immunoglobulin)/HSA (human serum albumin) mixture. Along with a high purity of 100%, a high IgG binding capacity of 30, 24, 21 and 28 mg mL(-1) (membrane volume) was achieved by the CEX, Me-affinity, His-affinity and Pep-affinity membrane adsorbers in flow-through mode respectively, which is superior to those of the reported membrane adsorbers. Furthermore, the CEX and Pep-affinity adsorbers were tested for the separation of IgG from human plasma solution. The carboxylic groups along with the peptides on the Pep-affinity adsorber captured IgG synergistically with a higher recovery and purity (99% and 98.6%). This synergetic adsorber showed a q(max) and dynamic binding capacity (DBC) of 138.4 and 38.7 mg mL(-1) respectively for IgG binding which was somewhat higher than that of the reported protein A agarose (having q(max) and DBC of 84 and 24.4 mg g(-1) respectively). The present work indicated that the ADA layer not only activated the membrane surface to attach various adsorptive ligands under mild conditions, but also reduced non-specific adsorption. Due to the versatile linking function and green reaction conditions, the ADA coating on the nylon membrane is promising for the preparation of diverse membrane adsorbers.
文章类型Article
WOS标题词Science & Technology ; Technology
DOI10.1039/c7tb02966g
收录类别SCI
语种英语
关键词[WOS]MONOCLONAL-ANTIBODY ; AFFINITY-CHROMATOGRAPHY ; PROTEIN SEPARATION ; PEPTIDE LIGANDS ; PURIFICATION ; IGG ; ADSORPTION
WOS研究方向Materials Science
WOS类目Materials Science, Biomaterials
项目资助者National Science Foundation of China(21506229) ; Youth Innovation Promotion Association of Chinese Academy of Sciences(2017069) ; '100 Talents Program' of the Chinese Academy of Sciences
WOS记录号WOS:000428186200009
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ipe.ac.cn/handle/122111/24066
专题生化工程国家重点实验室
作者单位1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
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Khan, M. Kamran,Luo, Jianquan,Wang, Zhaoshuai,et al. Alginate dialdehyde meets nylon membrane: a versatile platform for facile and green fabrication of membrane adsorbers[J]. JOURNAL OF MATERIALS CHEMISTRY B,2018,6(11):1640-1649.
APA Khan, M. Kamran,Luo, Jianquan,Wang, Zhaoshuai,Khan, Rashid,Chen, Xiangrong,&Wan, Yinhua.(2018).Alginate dialdehyde meets nylon membrane: a versatile platform for facile and green fabrication of membrane adsorbers.JOURNAL OF MATERIALS CHEMISTRY B,6(11),1640-1649.
MLA Khan, M. Kamran,et al."Alginate dialdehyde meets nylon membrane: a versatile platform for facile and green fabrication of membrane adsorbers".JOURNAL OF MATERIALS CHEMISTRY B 6.11(2018):1640-1649.
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