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Alginate dialdehyde meets nylon membrane: a versatile platform for facile and green fabrication of membrane adsorbers
Khan, M. Kamran1,2; Luo, Jianquan1,2; Wang, Zhaoshuai1,2; Khan, Rashid1,2; Chen, Xiangrong1; Wan, Yinhua1,2
2018-03-21
Source PublicationJOURNAL OF MATERIALS CHEMISTRY B
ISSN2050-750X
Volume6Issue:11Pages:1640-1649
AbstractAlginate dialdehyde (ADA), a biocompatible polymer, was used as an intermediate layer on a nylon membrane to readily fabricate cation exchange (CEX), metal-affinity (Me-affinity), histidine-affinity (His-affinity) and peptide-affinity (Pep-affinity) membrane adsorbers without any organic solvent usage. All the membrane adsorbers exhibited a high selectivity in the fractionation of a IgG (immunoglobulin)/HSA (human serum albumin) mixture. Along with a high purity of 100%, a high IgG binding capacity of 30, 24, 21 and 28 mg mL(-1) (membrane volume) was achieved by the CEX, Me-affinity, His-affinity and Pep-affinity membrane adsorbers in flow-through mode respectively, which is superior to those of the reported membrane adsorbers. Furthermore, the CEX and Pep-affinity adsorbers were tested for the separation of IgG from human plasma solution. The carboxylic groups along with the peptides on the Pep-affinity adsorber captured IgG synergistically with a higher recovery and purity (99% and 98.6%). This synergetic adsorber showed a q(max) and dynamic binding capacity (DBC) of 138.4 and 38.7 mg mL(-1) respectively for IgG binding which was somewhat higher than that of the reported protein A agarose (having q(max) and DBC of 84 and 24.4 mg g(-1) respectively). The present work indicated that the ADA layer not only activated the membrane surface to attach various adsorptive ligands under mild conditions, but also reduced non-specific adsorption. Due to the versatile linking function and green reaction conditions, the ADA coating on the nylon membrane is promising for the preparation of diverse membrane adsorbers.
SubtypeArticle
WOS HeadingsScience & Technology ; Technology
DOI10.1039/c7tb02966g
Indexed BySCI
Language英语
WOS KeywordMONOCLONAL-ANTIBODY ; AFFINITY-CHROMATOGRAPHY ; PROTEIN SEPARATION ; PEPTIDE LIGANDS ; PURIFICATION ; IGG ; ADSORPTION
WOS Research AreaMaterials Science
WOS SubjectMaterials Science, Biomaterials
Funding OrganizationNational Science Foundation of China(21506229) ; Youth Innovation Promotion Association of Chinese Academy of Sciences(2017069) ; '100 Talents Program' of the Chinese Academy of Sciences
WOS IDWOS:000428186200009
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/24066
Collection生化工程国家重点实验室
Affiliation1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Khan, M. Kamran,Luo, Jianquan,Wang, Zhaoshuai,et al. Alginate dialdehyde meets nylon membrane: a versatile platform for facile and green fabrication of membrane adsorbers[J]. JOURNAL OF MATERIALS CHEMISTRY B,2018,6(11):1640-1649.
APA Khan, M. Kamran,Luo, Jianquan,Wang, Zhaoshuai,Khan, Rashid,Chen, Xiangrong,&Wan, Yinhua.(2018).Alginate dialdehyde meets nylon membrane: a versatile platform for facile and green fabrication of membrane adsorbers.JOURNAL OF MATERIALS CHEMISTRY B,6(11),1640-1649.
MLA Khan, M. Kamran,et al."Alginate dialdehyde meets nylon membrane: a versatile platform for facile and green fabrication of membrane adsorbers".JOURNAL OF MATERIALS CHEMISTRY B 6.11(2018):1640-1649.
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