CAS OpenIR  > 生化工程国家重点实验室
Effect of Porogen Solubility Parameter on Structure of Chromatographic Supports with Large Pores
Lan Meng-Fei1; An Ning1; Zhao Ying1; Cao Wei1; Li Heng1; Zhao Lan2; Huang Yong-Dong2; Zhang Rong-Yue1
2018-02-01
Source PublicationCHINESE JOURNAL OF ANALYTICAL CHEMISTRY
ISSN0253-3820
Volume46Issue:2Pages:288-292
AbstractThe macroporous microspheres were prepared through suspension polymerization and based on a copolymer of glycidyl methacrylate and ethylene glycol dimethacrylate. The effect of porogen on the microspheres structure was evaluated in terms of pore size and surface area. Porogen contained dichloromethane (delta = 9.7 (cal/cm(3))(1/2)) and N-octanol (delta = 10.3 (cal/cm(3))(1/2)) which corresponded to a good and poor solvent, respectively. The solubility parameter of porogen was controlled in the range of 9.89-10.09 (cal/cm(3))(1/2). The pore size of microspheres increased with the difference value of solubility parameter between the polymer and the porogen. On the contrary, the surface area of microspheres decreased in this study. The anion exchange media was prepared through coupling poly(ethylene imine) in the microspheres, and the proteins transport was determined by frontal analysis method. The macroporous microspheres with 257 nm pore size could still afford a high proteins capacity (45.1 mg/mL). These macroporous supports showed a large potential in a rapid separation of proteins.
KeywordPorogen Solubility Parameter Macroporous Microspheres Protein Separation
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
DOI10.11895/j.issn.0253-3820.171299
Indexed BySCI
Language英语
WOS KeywordMICELLES SWELLING METHOD ; PROTEIN CHROMATOGRAPHY ; MICROSPHERES ; ADSORPTION ; MEDIA ; MODE
WOS Research AreaChemistry
WOS SubjectChemistry, Analytical
Funding OrganizationBeijing Natural Science Foundation, China(2162013)
WOS IDWOS:000427611700020
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/24106
Collection生化工程国家重点实验室
Affiliation1.Beijing Inst Petrochem Technol, Coll Chem Engn, Beijing Key Lab Fuels Cleaning & Adv Catalyt Emis, Beijing 102617, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Lan Meng-Fei,An Ning,Zhao Ying,et al. Effect of Porogen Solubility Parameter on Structure of Chromatographic Supports with Large Pores[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2018,46(2):288-292.
APA Lan Meng-Fei.,An Ning.,Zhao Ying.,Cao Wei.,Li Heng.,...&Zhang Rong-Yue.(2018).Effect of Porogen Solubility Parameter on Structure of Chromatographic Supports with Large Pores.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,46(2),288-292.
MLA Lan Meng-Fei,et al."Effect of Porogen Solubility Parameter on Structure of Chromatographic Supports with Large Pores".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 46.2(2018):288-292.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Lan Meng-Fei]'s Articles
[An Ning]'s Articles
[Zhao Ying]'s Articles
Baidu academic
Similar articles in Baidu academic
[Lan Meng-Fei]'s Articles
[An Ning]'s Articles
[Zhao Ying]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Lan Meng-Fei]'s Articles
[An Ning]'s Articles
[Zhao Ying]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.