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Effect of Porogen Solubility Parameter on Structure of Chromatographic Supports with Large Pores
Lan Meng-Fei1; An Ning1; Zhao Ying1; Cao Wei1; Li Heng1; Zhao Lan2; Huang Yong-Dong2; Zhang Rong-Yue1
2018-02-01
发表期刊CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
ISSN0253-3820
卷号46期号:2页码:288-292
摘要The macroporous microspheres were prepared through suspension polymerization and based on a copolymer of glycidyl methacrylate and ethylene glycol dimethacrylate. The effect of porogen on the microspheres structure was evaluated in terms of pore size and surface area. Porogen contained dichloromethane (delta = 9.7 (cal/cm(3))(1/2)) and N-octanol (delta = 10.3 (cal/cm(3))(1/2)) which corresponded to a good and poor solvent, respectively. The solubility parameter of porogen was controlled in the range of 9.89-10.09 (cal/cm(3))(1/2). The pore size of microspheres increased with the difference value of solubility parameter between the polymer and the porogen. On the contrary, the surface area of microspheres decreased in this study. The anion exchange media was prepared through coupling poly(ethylene imine) in the microspheres, and the proteins transport was determined by frontal analysis method. The macroporous microspheres with 257 nm pore size could still afford a high proteins capacity (45.1 mg/mL). These macroporous supports showed a large potential in a rapid separation of proteins.
关键词Porogen Solubility Parameter Macroporous Microspheres Protein Separation
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
DOI10.11895/j.issn.0253-3820.171299
收录类别SCI
语种英语
关键词[WOS]MICELLES SWELLING METHOD ; PROTEIN CHROMATOGRAPHY ; MICROSPHERES ; ADSORPTION ; MEDIA ; MODE
WOS研究方向Chemistry
WOS类目Chemistry, Analytical
项目资助者Beijing Natural Science Foundation, China(2162013)
WOS记录号WOS:000427611700020
引用统计
文献类型期刊论文
条目标识符http://ir.ipe.ac.cn/handle/122111/24106
专题生化工程国家重点实验室
作者单位1.Beijing Inst Petrochem Technol, Coll Chem Engn, Beijing Key Lab Fuels Cleaning & Adv Catalyt Emis, Beijing 102617, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
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Lan Meng-Fei,An Ning,Zhao Ying,et al. Effect of Porogen Solubility Parameter on Structure of Chromatographic Supports with Large Pores[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2018,46(2):288-292.
APA Lan Meng-Fei.,An Ning.,Zhao Ying.,Cao Wei.,Li Heng.,...&Zhang Rong-Yue.(2018).Effect of Porogen Solubility Parameter on Structure of Chromatographic Supports with Large Pores.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,46(2),288-292.
MLA Lan Meng-Fei,et al."Effect of Porogen Solubility Parameter on Structure of Chromatographic Supports with Large Pores".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 46.2(2018):288-292.
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