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A Highly Efficient Xylan-Utilization System in Aspergillus niger An76: A Functional-Proteomics Study
Gong, Weili1; Dai, Lin1; Zhang, Huaiqiang1; Zhang, Lili1; Wang, Lushan1,2
2018-03-22
发表期刊FRONTIERS IN MICROBIOLOGY
ISSN1664-302X
卷号9
摘要Xylan constituted with beta-1,4-D-xylose linked backbone and diverse substituted side-chains is the most abundant hemicellulose component of biomass, which can be completely and rapidly degraded into fermentable sugars by Aspergillus niger. This is of great value for obtaining renewable biofuels and biochemicals. To clarify the underlying mechanisms associated with highly efficient xylan degradation, assimilation, and metabolism by A. niger, we utilized functional proteomics to analyze the secreted proteins, sugar transporters, and intracellular proteins of A. niger An76 grown on xylan-based substrates. Results demonstrated that the complete xylanolytic enzyme system required for xylan degradation and composed of diverse isozymes was secreted in a sequential order. Xylan-backbone-degrading enzymes were preferentially induced by xylose or other soluble sugars, which efficiently produced large amounts of xylooligosaccharides (XOS) and xylose; however, XOS was more efficient than xylose in triggering the expression of the key transcription activator XInR, resulting in higher xylanase activity and shortening xylanase production time. Moreover, the substituted XOS was responsible for improving the abundance of side-chain-degrading enzymes, specific transporters, and key reductases and dehydrogenases in the pentose catabolic pathway. Our findings indicated that industries might be able to improve the species and concentrations of xylan-degrading enzymes and shorten fermentation time by adding abundant intermediate products of natural xylan (XOS) to cultures of filamentous fungi.
关键词Xylan-degrading Isoenzyme Sugar Transporter Transcription Activator Xinr Aspergillus Niger An76 Xylan
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine
DOI10.3389/fmicb.2018.00430
收录类别SCI
语种英语
关键词[WOS]ASSISTED CARBOHYDRATE ELECTROPHORESIS ; TRANSCRIPTIONAL ACTIVATOR XLNR ; TRICHODERMA-REESEI ; SHOTGUN PROTEOMICS ; INDUSTRIAL APPLICATIONS ; PENICILLIUM-OXALICUM ; MICROBIAL XYLANASES ; NEUROSPORA-CRASSA ; GENE-EXPRESSION ; DEGRADATION
WOS研究方向Microbiology
WOS类目Microbiology
项目资助者National Program on Key Research and Development Program of China(2016YFD080061) ; Natural Science Foundation of Shandong Province(ZR2013CM038) ; Open Funding Project of the State Key Laboratory of Biochemical Engineering(2015KF-05)
WOS记录号WOS:000428050500001
引用统计
文献类型期刊论文
条目标识符http://ir.ipe.ac.cn/handle/122111/24113
专题生化工程国家重点实验室
作者单位1.Shandong Univ, State Key Lab Microbial Technol, Jinan, Shandong, Peoples R China
2.Chinese Acad Sci, State Key Lab Biochem Engn, Inst Proc Engn, Beijing, Peoples R China
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Gong, Weili,Dai, Lin,Zhang, Huaiqiang,et al. A Highly Efficient Xylan-Utilization System in Aspergillus niger An76: A Functional-Proteomics Study[J]. FRONTIERS IN MICROBIOLOGY,2018,9.
APA Gong, Weili,Dai, Lin,Zhang, Huaiqiang,Zhang, Lili,&Wang, Lushan.(2018).A Highly Efficient Xylan-Utilization System in Aspergillus niger An76: A Functional-Proteomics Study.FRONTIERS IN MICROBIOLOGY,9.
MLA Gong, Weili,et al."A Highly Efficient Xylan-Utilization System in Aspergillus niger An76: A Functional-Proteomics Study".FRONTIERS IN MICROBIOLOGY 9(2018).
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