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A Highly Efficient Xylan-Utilization System in Aspergillus niger An76: A Functional-Proteomics Study
Gong, Weili1; Dai, Lin1; Zhang, Huaiqiang1; Zhang, Lili1; Wang, Lushan1,2
2018-03-22
Source PublicationFRONTIERS IN MICROBIOLOGY
ISSN1664-302X
Volume9
Abstract

Xylan constituted with beta-1,4-D-xylose linked backbone and diverse substituted side-chains is the most abundant hemicellulose component of biomass, which can be completely and rapidly degraded into fermentable sugars by Aspergillus niger. This is of great value for obtaining renewable biofuels and biochemicals. To clarify the underlying mechanisms associated with highly efficient xylan degradation, assimilation, and metabolism by A. niger, we utilized functional proteomics to analyze the secreted proteins, sugar transporters, and intracellular proteins of A. niger An76 grown on xylan-based substrates. Results demonstrated that the complete xylanolytic enzyme system required for xylan degradation and composed of diverse isozymes was secreted in a sequential order. Xylan-backbone-degrading enzymes were preferentially induced by xylose or other soluble sugars, which efficiently produced large amounts of xylooligosaccharides (XOS) and xylose; however, XOS was more efficient than xylose in triggering the expression of the key transcription activator XInR, resulting in higher xylanase activity and shortening xylanase production time. Moreover, the substituted XOS was responsible for improving the abundance of side-chain-degrading enzymes, specific transporters, and key reductases and dehydrogenases in the pentose catabolic pathway. Our findings indicated that industries might be able to improve the species and concentrations of xylan-degrading enzymes and shorten fermentation time by adding abundant intermediate products of natural xylan (XOS) to cultures of filamentous fungi.

KeywordXylan-degrading Isoenzyme Sugar Transporter Transcription Activator Xinr Aspergillus Niger An76 Xylan
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.3389/fmicb.2018.00430
Indexed BySCI
Language英语
WOS KeywordAssisted Carbohydrate Electrophoresis ; Transcriptional Activator Xlnr ; Trichoderma-reesei ; Shotgun Proteomics ; Industrial Applications ; Penicillium-oxalicum ; Microbial Xylanases ; Neurospora-crassa ; Gene-expression ; Degradation
WOS Research AreaMicrobiology
WOS SubjectMicrobiology
Funding OrganizationNational Program on Key Research and Development Program of China(2016YFD080061) ; Natural Science Foundation of Shandong Province(ZR2013CM038) ; Open Funding Project of the State Key Laboratory of Biochemical Engineering(2015KF-05)
WOS IDWOS:000428050500001
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/24113
Collection生化工程国家重点实验室
Affiliation1.Shandong Univ, State Key Lab Microbial Technol, Jinan, Shandong, Peoples R China
2.Chinese Acad Sci, State Key Lab Biochem Engn, Inst Proc Engn, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Gong, Weili,Dai, Lin,Zhang, Huaiqiang,et al. A Highly Efficient Xylan-Utilization System in Aspergillus niger An76: A Functional-Proteomics Study[J]. FRONTIERS IN MICROBIOLOGY,2018,9.
APA Gong, Weili,Dai, Lin,Zhang, Huaiqiang,Zhang, Lili,&Wang, Lushan.(2018).A Highly Efficient Xylan-Utilization System in Aspergillus niger An76: A Functional-Proteomics Study.FRONTIERS IN MICROBIOLOGY,9.
MLA Gong, Weili,et al."A Highly Efficient Xylan-Utilization System in Aspergillus niger An76: A Functional-Proteomics Study".FRONTIERS IN MICROBIOLOGY 9(2018).
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