| Biocatalytic Membrane Based on Polydopamine Coating: A Platform for Studying Immobilization Mechanisms | |
| Zhang, Huiru1,2; Luo, Jianquan1,2; Li, Sushuang1,2; Wei, Yuping1,2; Wan, Yinhua1,2 | |
| 2018-02-27 | |
| 发表期刊 | LANGMUIR
 |
| ISSN | 0743-7463 |
| 卷号 | 34期号:8页码:2585-2594 |
| 摘要 | Application of biocatalytic membrane is promising in food, pharmaceutical, and water treatment industries, whereas enzyme immobilization is the key step of biocatalytic membrane preparation. Thus, how to minimize the negative effect of immobilization on enzyme performance is required to answer. In this work, we proposed a platform for biocatalytic membrane preparation and immobilization mechanism investigation based on polydopamine (PDA) coating, which was demonstrated by immobilizing five commonly used enzymes (lactase, glucose oxidase, lipase, pepsin, and dextranase) on three commercially available membranes via three immobilization mechanisms (electrostatic attraction, covalent bonding, and hydrophobic adsorption), respectively. By examining the enzyme loading, activity, and kinetics under different immobilization mechanisms, we found that except for dextranase, enzyme immobilization via electrostatic attraction retained the most activity, whereas covalent bonding and hydrophobic adsorption were detrimental to enzyme conformation. Enzyme immobilization via covalent bonding ensured a high enzyme loading, and hydrophobic adsorption was only suitable for lipase and dextranase immobilization. Moreover, the properties of functional groups around the enzyme active center should be considered for the selection of suitable immobilization strategy (i.e., avoid covering the active center by membrane carrier). This work not only established a versatile platform for biocatalytic membrane preparation but also provided a novel methodology to evaluate the effect of immobilization mechanisms on enzyme performance. |
| 文章类型 | Article |
| WOS标题词 | Science & Technology ; Physical Sciences ; Technology |
| DOI | 10.1021/acs.langmuir.7b02860 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | ENZYME IMMOBILIZATION ; TRAMETES-VERSICOLOR ; SPACER ARM ; PURIFICATION ; LIPASE ; PERFORMANCE ; DEXTRANASE ; STRATEGIES ; SEPARATION ; NANOTUBES |
| WOS研究方向 | Chemistry ; Materials Science |
| WOS类目 | Chemistry, Multidisciplinary ; Chemistry, Physical ; Materials Science, Multidisciplinary |
| 项目资助者 | National Science Foundation of China(21506229) ; Youth Innovation Promotion Association of Chinese Academy of Sciences(2017069) ; "100 Talents Program" of the Chinese Academy of Sciences |
| WOS记录号 | WOS:000426614100001 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.ipe.ac.cn/handle/122111/24117 |
| 专题 | 生化工程国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Huiru,Luo, Jianquan,Li, Sushuang,et al. Biocatalytic Membrane Based on Polydopamine Coating: A Platform for Studying Immobilization Mechanisms[J]. LANGMUIR,2018,34(8):2585-2594. |
| APA | Zhang, Huiru,Luo, Jianquan,Li, Sushuang,Wei, Yuping,&Wan, Yinhua.(2018).Biocatalytic Membrane Based on Polydopamine Coating: A Platform for Studying Immobilization Mechanisms.LANGMUIR,34(8),2585-2594. |
| MLA | Zhang, Huiru,et al."Biocatalytic Membrane Based on Polydopamine Coating: A Platform for Studying Immobilization Mechanisms".LANGMUIR 34.8(2018):2585-2594. |
| 条目包含的文件 | 条目无相关文件。 | |||||
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