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Biocatalytic Membrane Based on Polydopamine Coating: A Platform for Studying Immobilization Mechanisms
Zhang, Huiru1,2; Luo, Jianquan1,2; Li, Sushuang1,2; Wei, Yuping1,2; Wan, Yinhua1,2
2018-02-27
发表期刊LANGMUIR
ISSN0743-7463
卷号34期号:8页码:2585-2594
摘要Application of biocatalytic membrane is promising in food, pharmaceutical, and water treatment industries, whereas enzyme immobilization is the key step of biocatalytic membrane preparation. Thus, how to minimize the negative effect of immobilization on enzyme performance is required to answer. In this work, we proposed a platform for biocatalytic membrane preparation and immobilization mechanism investigation based on polydopamine (PDA) coating, which was demonstrated by immobilizing five commonly used enzymes (lactase, glucose oxidase, lipase, pepsin, and dextranase) on three commercially available membranes via three immobilization mechanisms (electrostatic attraction, covalent bonding, and hydrophobic adsorption), respectively. By examining the enzyme loading, activity, and kinetics under different immobilization mechanisms, we found that except for dextranase, enzyme immobilization via electrostatic attraction retained the most activity, whereas covalent bonding and hydrophobic adsorption were detrimental to enzyme conformation. Enzyme immobilization via covalent bonding ensured a high enzyme loading, and hydrophobic adsorption was only suitable for lipase and dextranase immobilization. Moreover, the properties of functional groups around the enzyme active center should be considered for the selection of suitable immobilization strategy (i.e., avoid covering the active center by membrane carrier). This work not only established a versatile platform for biocatalytic membrane preparation but also provided a novel methodology to evaluate the effect of immobilization mechanisms on enzyme performance.
文章类型Article
WOS标题词Science & Technology ; Physical Sciences ; Technology
DOI10.1021/acs.langmuir.7b02860
收录类别SCI
语种英语
关键词[WOS]ENZYME IMMOBILIZATION ; TRAMETES-VERSICOLOR ; SPACER ARM ; PURIFICATION ; LIPASE ; PERFORMANCE ; DEXTRANASE ; STRATEGIES ; SEPARATION ; NANOTUBES
WOS研究方向Chemistry ; Materials Science
WOS类目Chemistry, Multidisciplinary ; Chemistry, Physical ; Materials Science, Multidisciplinary
项目资助者National Science Foundation of China(21506229) ; Youth Innovation Promotion Association of Chinese Academy of Sciences(2017069) ; "100 Talents Program" of the Chinese Academy of Sciences
WOS记录号WOS:000426614100001
引用统计
文献类型期刊论文
条目标识符http://ir.ipe.ac.cn/handle/122111/24117
专题生化工程国家重点实验室
作者单位1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
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Zhang, Huiru,Luo, Jianquan,Li, Sushuang,et al. Biocatalytic Membrane Based on Polydopamine Coating: A Platform for Studying Immobilization Mechanisms[J]. LANGMUIR,2018,34(8):2585-2594.
APA Zhang, Huiru,Luo, Jianquan,Li, Sushuang,Wei, Yuping,&Wan, Yinhua.(2018).Biocatalytic Membrane Based on Polydopamine Coating: A Platform for Studying Immobilization Mechanisms.LANGMUIR,34(8),2585-2594.
MLA Zhang, Huiru,et al."Biocatalytic Membrane Based on Polydopamine Coating: A Platform for Studying Immobilization Mechanisms".LANGMUIR 34.8(2018):2585-2594.
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