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Biocatalytic Membrane Based on Polydopamine Coating: A Platform for Studying Immobilization Mechanisms
Zhang, Huiru1,2; Luo, Jianquan1,2; Li, Sushuang1,2; Wei, Yuping1,2; Wan, Yinhua1,2
2018-02-27
Source PublicationLANGMUIR
ISSN0743-7463
Volume34Issue:8Pages:2585-2594
AbstractApplication of biocatalytic membrane is promising in food, pharmaceutical, and water treatment industries, whereas enzyme immobilization is the key step of biocatalytic membrane preparation. Thus, how to minimize the negative effect of immobilization on enzyme performance is required to answer. In this work, we proposed a platform for biocatalytic membrane preparation and immobilization mechanism investigation based on polydopamine (PDA) coating, which was demonstrated by immobilizing five commonly used enzymes (lactase, glucose oxidase, lipase, pepsin, and dextranase) on three commercially available membranes via three immobilization mechanisms (electrostatic attraction, covalent bonding, and hydrophobic adsorption), respectively. By examining the enzyme loading, activity, and kinetics under different immobilization mechanisms, we found that except for dextranase, enzyme immobilization via electrostatic attraction retained the most activity, whereas covalent bonding and hydrophobic adsorption were detrimental to enzyme conformation. Enzyme immobilization via covalent bonding ensured a high enzyme loading, and hydrophobic adsorption was only suitable for lipase and dextranase immobilization. Moreover, the properties of functional groups around the enzyme active center should be considered for the selection of suitable immobilization strategy (i.e., avoid covering the active center by membrane carrier). This work not only established a versatile platform for biocatalytic membrane preparation but also provided a novel methodology to evaluate the effect of immobilization mechanisms on enzyme performance.
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences ; Technology
DOI10.1021/acs.langmuir.7b02860
Indexed BySCI
Language英语
WOS KeywordENZYME IMMOBILIZATION ; TRAMETES-VERSICOLOR ; SPACER ARM ; PURIFICATION ; LIPASE ; PERFORMANCE ; DEXTRANASE ; STRATEGIES ; SEPARATION ; NANOTUBES
WOS Research AreaChemistry ; Materials Science
WOS SubjectChemistry, Multidisciplinary ; Chemistry, Physical ; Materials Science, Multidisciplinary
Funding OrganizationNational Science Foundation of China(21506229) ; Youth Innovation Promotion Association of Chinese Academy of Sciences(2017069) ; "100 Talents Program" of the Chinese Academy of Sciences
WOS IDWOS:000426614100001
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Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/24117
Collection生化工程国家重点实验室
Affiliation1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Huiru,Luo, Jianquan,Li, Sushuang,et al. Biocatalytic Membrane Based on Polydopamine Coating: A Platform for Studying Immobilization Mechanisms[J]. LANGMUIR,2018,34(8):2585-2594.
APA Zhang, Huiru,Luo, Jianquan,Li, Sushuang,Wei, Yuping,&Wan, Yinhua.(2018).Biocatalytic Membrane Based on Polydopamine Coating: A Platform for Studying Immobilization Mechanisms.LANGMUIR,34(8),2585-2594.
MLA Zhang, Huiru,et al."Biocatalytic Membrane Based on Polydopamine Coating: A Platform for Studying Immobilization Mechanisms".LANGMUIR 34.8(2018):2585-2594.
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