Identification of chitosan oligosaccharides binding proteins from the plasma membrane of wheat leaf cell

doi:10.1016/j.ijbiomac.2018.01.113

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	Identification of chitosan oligosaccharides binding proteins from the plasma membrane of wheat leaf cell
	Liu, Dongdong 1; Jiao, Siming 3; Cheng, Gong 3; Li, Xueming 1; Pei, Zhichao 1; Pei, Yuxin 1; Yin, Heng 2; Du, Yuguang 3
	2018-05-01
发表期刊	INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
ISSN	0141-8130
卷号	111 页码:1083-1090
摘要	Chitosan oligosaccharides (COS) have the ability to improve plant resistance to pests and diseases through activating plant immune system. However, it remains unclear whether stimulating reason of COS was associated with the plasma membrane proteins. Here, the interaction of COS with wheat leaf cell demonstrated that fluorescence-labeled COS were enriched on the cell surface and the interaction of COS with plasma membrane proteins was confirmed by quartz crystal microbalance (QCM) biosensor. What's more, HPLC and SDS-PAGE analysis showed that COS binding proteins exhibited more than three peaks and the molecular weight were 66 kDa to 97 kDa, where the COS binding proteins were fished out from wheat plasma membrane proteins by the COS affinity column. More importantly, LC-MS/MS analysis demonstrated that several candidates, including W5G2U8_WHEAT (a potential wall-associated receptor kinase protein), W5HY42_WHEAT and W510R4_WHEAT (potential G-type lectin S-receptor-like serine/threonine-protein kinase), have the potential to be COS receptors. (C) 2018 Elsevier B.V. All rights reserved.
关键词	Chitosan Oligosaccharides (Cos) Cos Binding Proteins Plasma Membrane Wheat Leaf Cell Plant Immune System
文章类型	Article
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI	10.1016/j.ijbiomac.2018.01.113
收录类别	SCI
语种	英语
关键词[WOS]	WALL-ASSOCIATED KINASES ; TOBACCO-MOSAIC-VIRUS ; PATTERN-RECOGNITION RECEPTORS ; CHITIN ELICITOR ; DEFENSE PROTEINS ; INNATE IMMUNITY ; PLANT-LECTINS ; H+-ATPASE ; ARABIDOPSIS ; RESISTANCE
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science
项目资助者	National Natural Science Foundation of China(NSFC21772157 ; Project of Science and Technology of Social Development in Shaanxi Province(2016SF-029) ; NSFC31100203)
WOS记录号	WOS:000429391000122
引用统计
文献类型	期刊论文
条目标识符	http://ir.ipe.ac.cn/handle/122111/24134
专题	生化工程国家重点实验室
作者单位	1.Northwest A&F Univ, Coll Chem & Pharm, Shaanxi Key Lab Nat Prod & Chem Biol, Yangling 712100, Shaanxi, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Nat Prod & Glycoconjugate Res Grp, Liaoning Prov Key Lab Carbohydrates, Dalian 116023, Peoples R China 3.Chinese Acad Sci, Natl Key Lab Biochem Engn, Natl Engn Res Ctr Biotechnol Beijing,Inst Proc En, Army Key Lab Biopharmaceut Mfg & Dosage Form Engn, 1 North 2nd St, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Liu, Dongdong,Jiao, Siming,Cheng, Gong,et al. Identification of chitosan oligosaccharides binding proteins from the plasma membrane of wheat leaf cell[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2018,111:1083-1090.
APA	Liu, Dongdong.,Jiao, Siming.,Cheng, Gong.,Li, Xueming.,Pei, Zhichao.,...&Du, Yuguang.(2018).Identification of chitosan oligosaccharides binding proteins from the plasma membrane of wheat leaf cell.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,111,1083-1090.
MLA	Liu, Dongdong,et al."Identification of chitosan oligosaccharides binding proteins from the plasma membrane of wheat leaf cell".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 111(2018):1083-1090.