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Identification of chitosan oligosaccharides binding proteins from the plasma membrane of wheat leaf cell
Liu, Dongdong1; Jiao, Siming3; Cheng, Gong3; Li, Xueming1; Pei, Zhichao1; Pei, Yuxin1; Yin, Heng2; Du, Yuguang3
2018-05-01
Source PublicationINTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
ISSN0141-8130
Volume111Pages:1083-1090
AbstractChitosan oligosaccharides (COS) have the ability to improve plant resistance to pests and diseases through activating plant immune system. However, it remains unclear whether stimulating reason of COS was associated with the plasma membrane proteins. Here, the interaction of COS with wheat leaf cell demonstrated that fluorescence-labeled COS were enriched on the cell surface and the interaction of COS with plasma membrane proteins was confirmed by quartz crystal microbalance (QCM) biosensor. What's more, HPLC and SDS-PAGE analysis showed that COS binding proteins exhibited more than three peaks and the molecular weight were 66 kDa to 97 kDa, where the COS binding proteins were fished out from wheat plasma membrane proteins by the COS affinity column. More importantly, LC-MS/MS analysis demonstrated that several candidates, including W5G2U8_WHEAT (a potential wall-associated receptor kinase protein), W5HY42_WHEAT and W510R4_WHEAT (potential G-type lectin S-receptor-like serine/threonine-protein kinase), have the potential to be COS receptors. (C) 2018 Elsevier B.V. All rights reserved.
KeywordChitosan Oligosaccharides (Cos) Cos Binding Proteins Plasma Membrane Wheat Leaf Cell Plant Immune System
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI10.1016/j.ijbiomac.2018.01.113
Indexed BySCI
Language英语
WOS KeywordWALL-ASSOCIATED KINASES ; TOBACCO-MOSAIC-VIRUS ; PATTERN-RECOGNITION RECEPTORS ; CHITIN ELICITOR ; DEFENSE PROTEINS ; INNATE IMMUNITY ; PLANT-LECTINS ; H+-ATPASE ; ARABIDOPSIS ; RESISTANCE
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry ; Polymer Science
WOS SubjectBiochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science
Funding OrganizationNational Natural Science Foundation of China(NSFC21772157 ; Project of Science and Technology of Social Development in Shaanxi Province(2016SF-029) ; NSFC31100203)
WOS IDWOS:000429391000122
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/24134
Collection生化工程国家重点实验室
Affiliation1.Northwest A&F Univ, Coll Chem & Pharm, Shaanxi Key Lab Nat Prod & Chem Biol, Yangling 712100, Shaanxi, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Nat Prod & Glycoconjugate Res Grp, Liaoning Prov Key Lab Carbohydrates, Dalian 116023, Peoples R China
3.Chinese Acad Sci, Natl Key Lab Biochem Engn, Natl Engn Res Ctr Biotechnol Beijing,Inst Proc En, Army Key Lab Biopharmaceut Mfg & Dosage Form Engn, 1 North 2nd St, Beijing 100190, Peoples R China
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GB/T 7714
Liu, Dongdong,Jiao, Siming,Cheng, Gong,et al. Identification of chitosan oligosaccharides binding proteins from the plasma membrane of wheat leaf cell[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2018,111:1083-1090.
APA Liu, Dongdong.,Jiao, Siming.,Cheng, Gong.,Li, Xueming.,Pei, Zhichao.,...&Du, Yuguang.(2018).Identification of chitosan oligosaccharides binding proteins from the plasma membrane of wheat leaf cell.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,111,1083-1090.
MLA Liu, Dongdong,et al."Identification of chitosan oligosaccharides binding proteins from the plasma membrane of wheat leaf cell".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 111(2018):1083-1090.
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