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Fabrication and characterization of DDAB/PLA-alginate composite microcapsules as single-shot vaccine
Yang, Meiyang1,2; Yang, Tingyuan1; Ji, Jilei1; Lu, Ting1; Wang, Hailin1,2; Yan, Xueying2; Wang, Lianyan1; Yu, Lian3; Zhao, Yue4
Source PublicationRSC ADVANCES

The most effective method to reduce chronic hepatitis B virus infection is the universal implementation of vaccination. The commercial aluminum-based vaccines need multiple-injection protocols for complete protection resulting in poor compliance in developing countries. It is necessary to develop single-shot vaccine formulations. In this study, novel antigen-loaded DDAB/PLA (didodecyldimethylammonium bromide/poly(lactic acid)) nanoparticles (NPs)-alginate composite microcapsules were developed as a single-shot vaccine. The hepatitis B surface antigen (HBsAg)-loaded DDAB/PLA NPs were successfully encapsulated into alginate microcapsules by a modified spray-solidification technique. The response surface method was applied to optimize the preparation parameters employing encapsulation efficiency of HBsAg and particle size of microcapsules as response variables. The antigen-loaded DDAB/PLA NPs-alginate composite microcapsules were prepared under these optimal conditions: the size of composite microcapsules was 24.25 m, the Span value was 1.627, and the encapsulation efficiency of HBsAg was 68.4%. The obtained microcapsules were spherical gel microparticles with excellent dispersity and narrow size distributions. In vitro release profile indicated a slow release rate of encapsulated HBsAg especially in phosphate buffered saline solution. The microcapsules showed little toxicity in vivo. This vaccine delivery system could induce stronger immune responses by a single shot, which exhibited much higher cytokine secretion levels closely related to cellular immunity and comparable IgG titers to the traditional aluminum-adjuvanted vaccine with three shots.

WOS HeadingsScience & Technology ; Physical Sciences
Indexed BySCI
WOS KeywordHepatitis-b ; Immune-responses ; Immunological Evaluation ; Aluminum Adjuvants ; Plga Microspheres ; Delivery ; Antigen ; Release ; Nanoparticles ; Technology
WOS Research AreaChemistry
WOS SubjectChemistry, Multidisciplinary
Funding OrganizationNational Science Foundation of China(21476243 ; National Science and Technology Major Project of China(2014ZX09102045-003) ; 81274101)
WOS IDWOS:000430018700064
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Document Type期刊论文
Affiliation1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Heilongjiang Univ Chinese Med, Harbin 150040, Heilongjiang, Peoples R China
3.Jiamusi Univ, Coll Pharm, Jiamusi 154000, Peoples R China
4.NHLBI, Epithelial Syst Biol Lab, Syst Biol Ctr, NIH, Bldg 10, Bethesda, MD 20892 USA
Recommended Citation
GB/T 7714
Yang, Meiyang,Yang, Tingyuan,Ji, Jilei,et al. Fabrication and characterization of DDAB/PLA-alginate composite microcapsules as single-shot vaccine[J]. RSC ADVANCES,2018,8(24):13612-13624.
APA Yang, Meiyang.,Yang, Tingyuan.,Ji, Jilei.,Lu, Ting.,Wang, Hailin.,...&Zhao, Yue.(2018).Fabrication and characterization of DDAB/PLA-alginate composite microcapsules as single-shot vaccine.RSC ADVANCES,8(24),13612-13624.
MLA Yang, Meiyang,et al."Fabrication and characterization of DDAB/PLA-alginate composite microcapsules as single-shot vaccine".RSC ADVANCES 8.24(2018):13612-13624.
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