| Fabrication and characterization of DDAB/PLA-alginate composite microcapsules as single-shot vaccine | |
| Yang, Meiyang1,2; Yang, Tingyuan1; Ji, Jilei1; Lu, Ting1; Wang, Hailin1,2; Yan, Xueying2; Wang, Lianyan1; Yu, Lian3; Zhao, Yue4 | |
| 2018 | |
| Source Publication | RSC ADVANCES
 |
| ISSN | 2046-2069 |
| Volume | 8Issue:24Pages:13612-13624 |
| Abstract | The most effective method to reduce chronic hepatitis B virus infection is the universal implementation of vaccination. The commercial aluminum-based vaccines need multiple-injection protocols for complete protection resulting in poor compliance in developing countries. It is necessary to develop single-shot vaccine formulations. In this study, novel antigen-loaded DDAB/PLA (didodecyldimethylammonium bromide/poly(lactic acid)) nanoparticles (NPs)-alginate composite microcapsules were developed as a single-shot vaccine. The hepatitis B surface antigen (HBsAg)-loaded DDAB/PLA NPs were successfully encapsulated into alginate microcapsules by a modified spray-solidification technique. The response surface method was applied to optimize the preparation parameters employing encapsulation efficiency of HBsAg and particle size of microcapsules as response variables. The antigen-loaded DDAB/PLA NPs-alginate composite microcapsules were prepared under these optimal conditions: the size of composite microcapsules was 24.25 m, the Span value was 1.627, and the encapsulation efficiency of HBsAg was 68.4%. The obtained microcapsules were spherical gel microparticles with excellent dispersity and narrow size distributions. In vitro release profile indicated a slow release rate of encapsulated HBsAg especially in phosphate buffered saline solution. The microcapsules showed little toxicity in vivo. This vaccine delivery system could induce stronger immune responses by a single shot, which exhibited much higher cytokine secretion levels closely related to cellular immunity and comparable IgG titers to the traditional aluminum-adjuvanted vaccine with three shots. |
| Subtype | Article |
| WOS Headings | Science & Technology ; Physical Sciences |
| DOI | 10.1039/c8ra00013a |
| Indexed By | SCI |
| Language | 英语 |
| WOS Keyword | HEPATITIS-B ; IMMUNE-RESPONSES ; IMMUNOLOGICAL EVALUATION ; ALUMINUM ADJUVANTS ; PLGA MICROSPHERES ; DELIVERY ; ANTIGEN ; RELEASE ; NANOPARTICLES ; TECHNOLOGY |
| WOS Research Area | Chemistry |
| WOS Subject | Chemistry, Multidisciplinary |
| Funding Organization | National Science Foundation of China(21476243 ; National Science and Technology Major Project of China(2014ZX09102045-003) ; 81274101) |
| WOS ID | WOS:000430018700064 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ipe.ac.cn/handle/122111/24153 |
| Collection | 生化工程国家重点实验室 |
| Affiliation | 1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Heilongjiang Univ Chinese Med, Harbin 150040, Heilongjiang, Peoples R China 3.Jiamusi Univ, Coll Pharm, Jiamusi 154000, Peoples R China 4.NHLBI, Epithelial Syst Biol Lab, Syst Biol Ctr, NIH, Bldg 10, Bethesda, MD 20892 USA |
| Recommended Citation GB/T 7714 | Yang, Meiyang,Yang, Tingyuan,Ji, Jilei,et al. Fabrication and characterization of DDAB/PLA-alginate composite microcapsules as single-shot vaccine[J]. RSC ADVANCES,2018,8(24):13612-13624. |
| APA | Yang, Meiyang.,Yang, Tingyuan.,Ji, Jilei.,Lu, Ting.,Wang, Hailin.,...&Zhao, Yue.(2018).Fabrication and characterization of DDAB/PLA-alginate composite microcapsules as single-shot vaccine.RSC ADVANCES,8(24),13612-13624. |
| MLA | Yang, Meiyang,et al."Fabrication and characterization of DDAB/PLA-alginate composite microcapsules as single-shot vaccine".RSC ADVANCES 8.24(2018):13612-13624. |
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