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Stereospecificity of Enoylreductase Domains from Modular Polyketide Synthases
Zhang, Luyun1,2; Ji, Junjie3; Yuan, Meijuan1,2; Feng, Yuanyuan1,2; Wang, Lei1,2; Deng, Zixin1,2; Bai, Linquan1,2; Zheng, Jianting1,2
2018-04-01
发表期刊ACS CHEMICAL BIOLOGY
ISSN1554-8929
卷号13期号:4页码:871-875
摘要An enoylreductase (ER) domain of a polyketide synthase module recruiting a methylmalonate extender unit sets the C2 methyl branch to either the S or R configuration during processing of a polyketide intermediate carried by an acyl carrier protein (ACP) domain. In the present study, pantetheine- and ACP-bound trans-2-methylcrotonyl substrate surrogates were used to scrutinize the stereospecificity of the ER domains. The pantetheine-bound thioester was reduced to mixtures of both 2R and 2S products, whereas the expected 2S epimer was almost exclusively generated when the cognate ACP-bound substrate surrogate was utilized. The analogous incubation of an ER with the substrate surrogate carried by a noncognate ACP significantly increased the generation of the unexpected 2R epimer, highlighting the dependence of stereospecificity on proper protein protein interactions between ER and ACP domains. The ER mutant assays revealed the involvement of the conserved tyrosine and lysine in stereocontrol. Taken together, these results expand the current understanding of the ER stereochemistry and help in the engineering of modular PKSs.
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine
DOI10.1021/acschembio.7b00982
收录类别SCI
语种英语
关键词[WOS]KETOREDUCTASE DOMAINS ; CARRIER PROTEIN ; STEREOCHEMISTRY ; BIOSYNTHESIS ; CATALYSIS ; ACYLTRANSFERASE ; MUTAGENESIS ; SUBSTRATE
WOS研究方向Biochemistry & Molecular Biology
WOS类目Biochemistry & Molecular Biology
项目资助者National Natural Science Foundation of China(31370101 ; National Program on Key Basic Research Project (973 program)(2013CB734002) ; State Key Laboratory of Pathogen and Biosecurity (Academy of Military Medical Science)(SKLPBS1526) ; Fundamental Research Funds for the Central Universities ; Global Talents Recruitment Program ; 31570056 ; 31770068)
WOS记录号WOS:000430898600004
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文献类型期刊论文
条目标识符http://ir.ipe.ac.cn/handle/122111/24436
专题生化工程国家重点实验室
作者单位1.Shanghai Jiao Tong Univ, State Key Lab Microbial Metab, Shanghai 200240, Peoples R China
2.Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, Shanghai 200240, Peoples R China
3.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
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Zhang, Luyun,Ji, Junjie,Yuan, Meijuan,et al. Stereospecificity of Enoylreductase Domains from Modular Polyketide Synthases[J]. ACS CHEMICAL BIOLOGY,2018,13(4):871-875.
APA Zhang, Luyun.,Ji, Junjie.,Yuan, Meijuan.,Feng, Yuanyuan.,Wang, Lei.,...&Zheng, Jianting.(2018).Stereospecificity of Enoylreductase Domains from Modular Polyketide Synthases.ACS CHEMICAL BIOLOGY,13(4),871-875.
MLA Zhang, Luyun,et al."Stereospecificity of Enoylreductase Domains from Modular Polyketide Synthases".ACS CHEMICAL BIOLOGY 13.4(2018):871-875.
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