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Effect of culture condition on the growth, biochemical composition and EPA production of alkaliphilic Nitzschia plea isolated in the Southeast of China
Zhang, Dongmei; Wen, Shumei; Wu, Xia; Cong, Wei
2018-06-01
Source PublicationBIOPROCESS AND BIOSYSTEMS ENGINEERING
ISSN1615-7591
Volume41Issue:6Pages:831-839
AbstractTo overcome the contamination in open pond, microalgal strain selection should focus on species with tolerability to extreme environments. In this study, a native alkaliphilic algae, diatom Nitzschia plea was obtained in Southeast of China, which could tolerate high concentration of NaHCO3 (0.15 mol/L) and high pH (> 10). The effects of initial pH, light intensity and temperature on cell growth, biochemical composition and fatty acid profile of N. plea were investigated. Results indicated its specific growth rate could reach 1.2 day(-1), lipid content was in the range 14.6-30.2% of dry weight, eicosapntemacnioc acid (EPA, C20:5) accounted for around 15% of total fatty acids. Alkalic condition benefited for both cell growth and EPA synthesis. Appropriately increasing light intensity and temperature could improve cell growth rate and lipid synthesis, although the proportion of EPA in total fatty acids decreased slightly. The optimal culture condition (pH 9.00, temperature 35.0 A degrees C, light intensity 158.6 A mu mol/m(2)s) was suggested for maximum yield of EPA based on the response surface model. The overall biomass productivity and EPA productivity were 0.301 g/L/day and 7.43 mg/L/day, respectively. In conclusion, alkalic environment was helpful for the steady operation of open pond cultivation of N. plea with the characteristics of fast growth rate and high EPA content, which exhibited its commercial value.
KeywordAlkaliphilic microalgae Eicosapntemacnioc acid Nitzschia plea Response surface
DOI10.1007/s00449-018-1917-0
Language英语
WOS KeywordSPIRULINA-PLATENSIS ; CHLORELLA-VULGARIS ; MARINE MICROALGAE ; CARBON-DIOXIDE ; TEMPERATURE ; ACCUMULATION ; BICARBONATE ; PURIFICATION ; DUNALIELLA ; DIVERSITY
Funding ProjectNational Key R&D Program of China[2016YF0601005]
WOS Research AreaBiotechnology & Applied Microbiology ; Engineering
WOS SubjectBiotechnology & Applied Microbiology ; Engineering, Chemical
Funding OrganizationNational Key R&D Program of China
WOS IDWOS:000432600500009
PublisherSPRINGER
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/24618
Collection中国科学院过程工程研究所
Corresponding AuthorCong, Wei
AffiliationChinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Dongmei,Wen, Shumei,Wu, Xia,et al. Effect of culture condition on the growth, biochemical composition and EPA production of alkaliphilic Nitzschia plea isolated in the Southeast of China[J]. BIOPROCESS AND BIOSYSTEMS ENGINEERING,2018,41(6):831-839.
APA Zhang, Dongmei,Wen, Shumei,Wu, Xia,&Cong, Wei.(2018).Effect of culture condition on the growth, biochemical composition and EPA production of alkaliphilic Nitzschia plea isolated in the Southeast of China.BIOPROCESS AND BIOSYSTEMS ENGINEERING,41(6),831-839.
MLA Zhang, Dongmei,et al."Effect of culture condition on the growth, biochemical composition and EPA production of alkaliphilic Nitzschia plea isolated in the Southeast of China".BIOPROCESS AND BIOSYSTEMS ENGINEERING 41.6(2018):831-839.
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