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The interacting role of physical stiffness and tumor cells on the macrophages polarization
Wu, Sihua1; Yue, Hua2; Wu, Jie2,4; Zhang, Wenming1,3; Jiang, Min1,3; Ma, Guanghui2,3,4
2018-09-05
Source PublicationCOLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
ISSN0927-7757
Volume552Pages:81-88
Abstract

There is a complex interplay existing between the tumor microenvironment and cellular function, while the influence of tumor biophysical signals on the macrophage function remain unclear. Herein, we constructed a three dimensional hydrogel model to study the symphysial effect of physical signal (stiffness) and biological signal (tumor cells) on macrophage polarization in different stage of tumor progression. In the absence of biological signal, the macrophage response was slightly changed with different stiffness (100 Pa, 300 Pa, and 900 Pa). Once co-incubated with the tumor cell, macrophage induced more significant cancer facilitated cytokines (e.g. IL-10, MMP-9) and chemokines (MCP-1). In contrast to the education role from the co-cultured cancer cells, a stiffness around the normal tissue (100 Pa, CO-L group) provided a physical cue for cancer cell regression. This cue triggered the active role of M1 type macrophages (e.g. IL-12), indicating the interacting role of stiffness in the presence of cancer cell. The stiffness around the premalignant tissue (M, 300 Pa) induced much higher level of cytokines (e.g. IL-6, IL-10, IL-12, CD86, and CD206), while the immune activation cytokines (M1 type) was largely attenuated at high stiffness (900 Pa). This study revealed the pleiotropic tuning of macrophage function under different biophysical signals, which provided important knowledge for cancer progress and may benefit the design of cancer-related therapies.

KeywordHydrogel Macrophages Tumor Environment Physical Signal
DOI10.1016/j.colsurfa.2018.04.026
Language英语
WOS KeywordExtracellular-matrix ; Substrate Stiffness ; Microenvironment ; Cancer ; Progression ; Activation ; Metastasis ; Phenotype ; Discovery ; Hydrogels
Funding ProjectNational Science and Technology Major Project[2014ZX09102045] ; National Natural Science Foundation of China[21706125] ; National Natural Science Foundation of China[21727818] ; National Natural Science Foundation of China[51302265] ; Jiangsu Synergetic Innovation Center for Advanced Bio-Manufacture of China ; Top-notch Academic Programs Project of Jiangsu Higher Education Institutions[PPZY2015B155] ; TAPP ; Youth Innovation Promotion Association, CAS[2013033]
WOS Research AreaChemistry
WOS SubjectChemistry, Physical
Funding OrganizationNational Science and Technology Major Project ; National Natural Science Foundation of China ; Jiangsu Synergetic Innovation Center for Advanced Bio-Manufacture of China ; Top-notch Academic Programs Project of Jiangsu Higher Education Institutions ; TAPP ; Youth Innovation Promotion Association, CAS
WOS IDWOS:000433221200011
PublisherELSEVIER SCIENCE BV
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/24654
Collection中国科学院过程工程研究所
Corresponding AuthorJiang, Min; Ma, Guanghui
Affiliation1.Nanjing Tech Univ, Coll Biotechnol & Pharmaceut Engn, State Key Lab Mat Oriented Chem Engn, Nanjing 211800, Jiangsu, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
3.Jiangsu Natl Synerget Innovat Ctr Adv Mat, Nanjing 211816, Jiangsu, Peoples R China
4.Chinese Acad Sci, Inst Proc Engn, PLA Key Lab Biopharmaceut Prod & Formulat Engn, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Wu, Sihua,Yue, Hua,Wu, Jie,et al. The interacting role of physical stiffness and tumor cells on the macrophages polarization[J]. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,2018,552:81-88.
APA Wu, Sihua,Yue, Hua,Wu, Jie,Zhang, Wenming,Jiang, Min,&Ma, Guanghui.(2018).The interacting role of physical stiffness and tumor cells on the macrophages polarization.COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,552,81-88.
MLA Wu, Sihua,et al."The interacting role of physical stiffness and tumor cells on the macrophages polarization".COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS 552(2018):81-88.
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