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Sandwiching multiple dehydrogenases and shared cofactor between double polyelectrolytes for enhanced communication of cofactor and enzymes
Ji, Xiaoyuan1,2; Su, Zhiguo1; Ma, Guanghui1; Zhang, Songping1
2018-09-15
Source PublicationBIOCHEMICAL ENGINEERING JOURNAL
ISSN1369-703X
Volume137Pages:40-49
Abstract

Regeneration and reutilization of cofactors remain as the biggest challenge facing the industrial application of cofactor-dependent redox, while efficient communication and synergism between enzymes and cofactors are the major requirements for implementation of such processed. Inspired by swinging arms facilitating multistep catalytic transformations in nature, polyelectrolytes-based artificial swinging arms were introduced to a multienzyme system by coaxial electrospinning technology. The poly(allylamine hydrochloride) (PAH), pre-dissolved in the core-phase solution, would penetrate across the shell of hollow nanofibers, thus act as artificial swinging arms and enable effective tethering and retention of the enzymes and cofactors on the outer surface of nanofibers via the ion-exchange interactions. With further intercalary insertion of another negatively charged polyelectrolyte poly(styrene sulfonate) (PSS), a sandwich-like structure was formed on the outer surface of the hollow nanofiber. It was found that PSS layer not only effectively prevented the leaking of enzymes and cofactors, the double-polyelectrolytes also imposed crowding and confinement effects on the sandwiched multienzymes, thus the communication of the dehydrogenases and the shared cofactors were further facilitated. The methanol yield obtained with the sandwiched multienzymes system reached to 98.27%, which was 2.72-fold higher than that of the free system, and even 1.52-fold higher than that without PSS layer. (C) 2018 Elsevier B.V. All rights reserved.

KeywordCofactor Dehydrogenase Artificial Swinging Arm Polyelectrolyte Methanol Conversion
DOI10.1016/j.bej.2018.05.017
Language英语
WOS KeywordCarbon-dioxide ; Hollow Nanofibers ; Protein Stability ; Bienzyme System ; Cascade ; Regeneration ; Confinement ; Methanol ; Environments ; Encapsulation
Funding ProjectNational Natural Science Foundation of China[21676276] ; National Natural Science Foundation of China[91534126] ; National Basic Research Program of China (973 Program)[2013CB733604]
WOS Research AreaBiotechnology & Applied Microbiology ; Engineering
WOS SubjectBiotechnology & Applied Microbiology ; Engineering, Chemical
Funding OrganizationNational Natural Science Foundation of China ; National Basic Research Program of China (973 Program)
WOS IDWOS:000442977000006
PublisherELSEVIER SCIENCE BV
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Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/25523
Collection中国科学院过程工程研究所
Corresponding AuthorZhang, Songping
Affiliation1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Sun Yat Sen Univ, Sch Pharmaceut Sci Shenzhen, Guangzhou 510275, Guangdong, Peoples R China
Recommended Citation
GB/T 7714
Ji, Xiaoyuan,Su, Zhiguo,Ma, Guanghui,et al. Sandwiching multiple dehydrogenases and shared cofactor between double polyelectrolytes for enhanced communication of cofactor and enzymes[J]. BIOCHEMICAL ENGINEERING JOURNAL,2018,137:40-49.
APA Ji, Xiaoyuan,Su, Zhiguo,Ma, Guanghui,&Zhang, Songping.(2018).Sandwiching multiple dehydrogenases and shared cofactor between double polyelectrolytes for enhanced communication of cofactor and enzymes.BIOCHEMICAL ENGINEERING JOURNAL,137,40-49.
MLA Ji, Xiaoyuan,et al."Sandwiching multiple dehydrogenases and shared cofactor between double polyelectrolytes for enhanced communication of cofactor and enzymes".BIOCHEMICAL ENGINEERING JOURNAL 137(2018):40-49.
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