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Simultaneous decolorization and deproteinization of alpha,omega-dodecanedioic acid fermentation broth by integrated ultrafiltration and adsorption treatments
Cao, Weifeng1; Wang, Yujue1,2; Luo, Jianquan1,2; Yin, Junxiang3; Wan, Yinhua1,2
2018-09-01
Source PublicationBIOPROCESS AND BIOSYSTEMS ENGINEERING
ISSN1615-7591
Volume41Issue:9Pages:1271-1281
Abstractalpha,omega-Dicarboxylic acids (DC) are versatile chemical intermediates with different chain length. For biosynthesis of DC, to obtain the highly pure product via crystallization, it is required to remove pigments and proteins in fermentation broth. However, a trade-off between decolorization/deproteinization ratio and DC recovery during the purification process was found, which impeded DC production by fermentation. When ultrafiltration (UF) was applied to treat alpha,omega-dodecanedioic acid (DC12) broth, 93.4% of DC12 recovery, 80.5% of decolorization ratio and 61.7% of deproteinization ratio were achieved by a PES 3 membrane. However, the membrane technology could not effectively retain the pigments or proteins with low molecular weight when a high DC12 permeation was required. Meanwhile, the selected activated charcoal or macroporous resins were not good adsorbents for the present system. Furthermore, an integrated process for decolorization and deproteinization was developed. After filtration with PES3 membrane, an activated charcoal was used to remove the small proteins and pigments in the UF permeate. As a result, 91.4% of DC12 recovery, 94.7% of decolorization ratio and 84.8% of deproteinization ratio were obtained by such two-stage strategy. These results would serve as a valuable guide for process design and practical operation in subsequent industrial application.
Keywordalpha,omega-Dodecanedioic acid Ultrafiltration Decolorization Deproteinization Adsorption
DOI10.1007/s00449-018-1955-7
Language英语
WOS KeywordCANDIDA-VISWANATHII ; DICARBOXYLIC-ACIDS ; ACTIVATED-CHARCOAL ; ALKALINE PROTEASE ; MEMBRANE ; PURIFICATION ; TEMPERATURE ; FILTRATION ; WATER
Funding ProjectNational High Technology Research and Development Program of China[2015AA021002] ; National High Technology Research and Development Program of China[2014AA021005] ; National Science Foundation of China[21406240]
WOS Research AreaBiotechnology & Applied Microbiology ; Engineering
WOS SubjectBiotechnology & Applied Microbiology ; Engineering, Chemical
Funding OrganizationNational High Technology Research and Development Program of China ; National Science Foundation of China
WOS IDWOS:000441931100004
PublisherSPRINGER
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Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/25567
Collection中国科学院过程工程研究所
Corresponding AuthorWan, Yinhua
Affiliation1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Chinese Acad Sci, Beijing 100049, Peoples R China
3.China Natl Ctr Biotechnol Dev, Beijing 100036, Peoples R China
Recommended Citation
GB/T 7714
Cao, Weifeng,Wang, Yujue,Luo, Jianquan,et al. Simultaneous decolorization and deproteinization of alpha,omega-dodecanedioic acid fermentation broth by integrated ultrafiltration and adsorption treatments[J]. BIOPROCESS AND BIOSYSTEMS ENGINEERING,2018,41(9):1271-1281.
APA Cao, Weifeng,Wang, Yujue,Luo, Jianquan,Yin, Junxiang,&Wan, Yinhua.(2018).Simultaneous decolorization and deproteinization of alpha,omega-dodecanedioic acid fermentation broth by integrated ultrafiltration and adsorption treatments.BIOPROCESS AND BIOSYSTEMS ENGINEERING,41(9),1271-1281.
MLA Cao, Weifeng,et al."Simultaneous decolorization and deproteinization of alpha,omega-dodecanedioic acid fermentation broth by integrated ultrafiltration and adsorption treatments".BIOPROCESS AND BIOSYSTEMS ENGINEERING 41.9(2018):1271-1281.
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