CAS OpenIR
Enhanced production of succinic acid from methanol-organosolv pretreated Strophanthus preussii by recombinant Escherichia coli
Olajuyin, Ayobami Matthew1,2; Yang, Maohua1; Mu, Tingzhen1,2; Tian, Jiangnan1,2; Thygesen, Anders3,4; Adesanoye, Omolola Abidemi5; Adaramoye, Oluwatosin Adekunle5; Song, Andong6; Xing, Jianmin1,2
2018-10-01
Source PublicationBIOPROCESS AND BIOSYSTEMS ENGINEERING
ISSN1615-7591
Volume41Issue:10Pages:1497-1508
Abstract

A biorefinery process for high yield production of succinic acid from biomass sugars was investigated using recombinant Escherichia coli. The major problem been addressed is utilization of waste biomass for the production of succinic acid using metabolic engineering strategy. Here, methanol extract of Strophanthus preussii was used for fermentation. The process parameters were ptimized. Glucose (9 g/L), galactose (4 g/L), xylose (6 g/L) and arabinose (0.5 g/L) were the major sugars present in the methanol extract of S. preussii. E. coli K3OS with overexpression of soluble nucleotide pyridine transhydrogenase sthA and mutation of lactate dehydrogenase A (ldhA), phosphotransacetylase acetate kinase A (pta-ackA), pyruvate formate lyase B (pflB), pyruvate oxidase B (poxB), produced a final succinic acid concentration of 14.40 g/L and yield of 1.10 mol/mol total sugars after 72 h dual-phase fermentation in M9 medium. Here, we show that the maximum theoretical yield using methanol extracts of S. preussii was 64%. Hence, methanol extract of S. preussii could be used for the production of biochemicals such as succinate, malate and pyruvate.

KeywordEscherichia Coli Succinic Acid Fermentation Strophanthus Preussii Biorefinery
DOI10.1007/s00449-018-1977-1
Language英语
WOS KeywordLignocellulosic Biomass ; Efficient Production ; Gene Knockout ; Enzymatic-hydrolysis ; Engineered Strains ; Glucose ; Overexpression ; Growth ; Dehydrogenase ; Deletion
Funding ProjectNational High Technology Research and Development Program of China (863 Project)[2014AA021905]
WOS Research AreaBiotechnology & Applied Microbiology ; Engineering
WOS SubjectBiotechnology & Applied Microbiology ; Engineering, Chemical
Funding OrganizationNational High Technology Research and Development Program of China (863 Project)
WOS IDWOS:000443552900009
PublisherSPRINGER
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/25837
Collection中国科学院过程工程研究所
Corresponding AuthorSong, Andong; Xing, Jianmin
Affiliation1.Inst Proc Engn, State Key Lab Biochem Engn, Beijing, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Tech Univ Denmark, Dept Chem & Biochem Engn, DK-2800 Lyngby, Denmark
4.Sinodanish Ctr Educ & Res, Niels Jensensvej 2, DK-8000 Aarhus C, Denmark
5.Univ Ibadan, Fac Basic Med Sci, Dept Biochem, Ibadan, Nigeria
6.Henan Agr Univ, Coll Life Sci, Key Lab Enzyme Engn Agr Microbiol, Minist Agr, Zhengzhou 450002, Henan, Peoples R China
Recommended Citation
GB/T 7714
Olajuyin, Ayobami Matthew,Yang, Maohua,Mu, Tingzhen,et al. Enhanced production of succinic acid from methanol-organosolv pretreated Strophanthus preussii by recombinant Escherichia coli[J]. BIOPROCESS AND BIOSYSTEMS ENGINEERING,2018,41(10):1497-1508.
APA Olajuyin, Ayobami Matthew.,Yang, Maohua.,Mu, Tingzhen.,Tian, Jiangnan.,Thygesen, Anders.,...&Xing, Jianmin.(2018).Enhanced production of succinic acid from methanol-organosolv pretreated Strophanthus preussii by recombinant Escherichia coli.BIOPROCESS AND BIOSYSTEMS ENGINEERING,41(10),1497-1508.
MLA Olajuyin, Ayobami Matthew,et al."Enhanced production of succinic acid from methanol-organosolv pretreated Strophanthus preussii by recombinant Escherichia coli".BIOPROCESS AND BIOSYSTEMS ENGINEERING 41.10(2018):1497-1508.
Files in This Item:
File Name/Size DocType Version Access License
Enhanced production (2669KB)期刊论文出版稿限制开放CC BY-NC-SAApplication Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Olajuyin, Ayobami Matthew]'s Articles
[Yang, Maohua]'s Articles
[Mu, Tingzhen]'s Articles
Baidu academic
Similar articles in Baidu academic
[Olajuyin, Ayobami Matthew]'s Articles
[Yang, Maohua]'s Articles
[Mu, Tingzhen]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Olajuyin, Ayobami Matthew]'s Articles
[Yang, Maohua]'s Articles
[Mu, Tingzhen]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.