CAS OpenIR
Immobilization of cellulase on a core-shell structured metal-organic framework composites: Better inhibitors tolerance and easier recycling
Qi, Benkun; Luo, Jianquan; Wan, Yinhua
2018-11-01
Source PublicationBIORESOURCE TECHNOLOGY
ISSN0960-8524
Volume268Pages:577-582
Abstract

For the first time, cellulase was successfully immobilized on a magnetic core-shell metal-organic framework (MOF) material, UIO-66-NH2. The as-prepared immobilized cellulase demonstrated a high protein loading efficiency of 126.2 g/g support and a high enzyme activity recovery of 78.4%. Cellulase immobilized on magnetic UIO-66-NH2 exhibited a superior performance in terms of pH stability, thermal stability and catalytic efficiency compared to its free form. Notably, immobilized cellulase could be recycled for up to 5 consecutive runs. Furthermore, compared to free cellulase, immobilized cellulase showed better tolerance to formic acid and vanillin, two typical inhibitors found in lignocellulosic prehydrolysates. In the presence of 5 g/L of formic acid and vanillin, immobilized cellulase demonstrated 16.8% and 21.5% higher activity than free enzyme, respectively, and its improvement in hydrolysis yield was 18.7% and 19.6% respectively. This is firstly confirmed that immobilization can alleviate the inhibitory effects of certain pretreatment inhibitors on cellulase.

KeywordMagnetic Metal-organic Frameworks Cellulase Immobilization Pretreatment Inhibitors
DOI10.1016/j.biortech.2018.07.115
Language英语
WOS KeywordCovalent Immobilization ; Magnetic Nanoparticles ; Enzyme-activity ; Lignocellulose ; Hydrolysis ; Particles ; Stability ; Support ; Biorefineries ; Biocatalyst
Funding ProjectNational Natural Science Foundation of China, China[21306211] ; National Key Research and Development Plan of China, United States[2017YFC1600906]
WOS Research AreaAgriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels
WOS SubjectAgricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels
Funding OrganizationNational Natural Science Foundation of China, China ; National Key Research and Development Plan of China, United States
WOS IDWOS:000445043600070
PublisherELSEVIER SCI LTD
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/25994
Collection中国科学院过程工程研究所
Corresponding AuthorWan, Yinhua
AffiliationChinese Acad Sci, Univ Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Qi, Benkun,Luo, Jianquan,Wan, Yinhua. Immobilization of cellulase on a core-shell structured metal-organic framework composites: Better inhibitors tolerance and easier recycling[J]. BIORESOURCE TECHNOLOGY,2018,268:577-582.
APA Qi, Benkun,Luo, Jianquan,&Wan, Yinhua.(2018).Immobilization of cellulase on a core-shell structured metal-organic framework composites: Better inhibitors tolerance and easier recycling.BIORESOURCE TECHNOLOGY,268,577-582.
MLA Qi, Benkun,et al."Immobilization of cellulase on a core-shell structured metal-organic framework composites: Better inhibitors tolerance and easier recycling".BIORESOURCE TECHNOLOGY 268(2018):577-582.
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