CAS OpenIR
Conjugation of chitosan oligosaccharides via a carrier protein markedly improves immunogenicity of porcine circovirus vaccine
Zhang, Guiqiang1,2,3; Jia, Peiyuan2,3; Liu, Hongtao2,3; Hu, Tao2,3; Du, Yuguang2,3
2018-10-01
Source PublicationGLYCOCONJUGATE JOURNAL
ISSN0282-0080
Volume35Issue:5Pages:451-459
Abstract

Porcine circovirus type 2 (PCV2)-associated diseases have led to huge economic losses in pig industry. Our laboratory previously found that conjugation of chitosan oligosaccharides (COS) enhanced the immunogenicity of PCV2 vaccine against infectious pathogens. In this study, an effective adjuvant system was developed by covalent conjugation of COS via a carrier protein (Ovalbumin, OVA) to further increase the immunogenicity of vaccine. Its effect on dendritic cells maturation was assessed in vitro and its immunogenicity was investigated in mice. The results indicated that, as compared to the PCV2 and COS-PCV2, COS-OVA-PCV2 stimulated dendritic cells to express higher maturation markers (CD80, CD86, CD40 and MHC class II) and remarkably promoted both humoral and cellular immunity against PCV2 by enhancing the lymphocyte proliferation and inducing a mixed Th1/Th2 response, including the increased production of PCV2-specific antibodies and raised levels of inflammatory cytokines. Furthermore, it displayed better immune-stimulating effects than the physical mixture of vaccine and ISA206 (a commercialized adjuvant). In conclusion, conjugation of COS via a carrier protein might be a promising strategy to enhance the immunogenicity of vaccines.

KeywordPorcine Circovirus Type 2 (Pcv2) Chitosan Oligosaccharide Conjugation Adjuvant System Carrier Protein
DOI10.1007/s10719-018-9830-y
Language英语
WOS KeywordHuman Dendritic Cells ; Glycoconjugate Vaccine ; Adaptive Immunity ; Polysaccharide ; Adjuvant ; Antigen ; Activation ; Delivery ; Innate
WOS Research AreaBiochemistry & Molecular Biology
WOS SubjectBiochemistry & Molecular Biology
WOS IDWOS:000446708500004
PublisherSPRINGER
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/26163
Collection中国科学院过程工程研究所
Corresponding AuthorLiu, Hongtao; Hu, Tao; Du, Yuguang
Affiliation1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, PLA, Key Lab Biopharmaceut Prod & Formulat Engn, 1 Bei Er Tiao, Beijing 100190, Peoples R China
3.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, 1 Bei Er Tiao, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Guiqiang,Jia, Peiyuan,Liu, Hongtao,et al. Conjugation of chitosan oligosaccharides via a carrier protein markedly improves immunogenicity of porcine circovirus vaccine[J]. GLYCOCONJUGATE JOURNAL,2018,35(5):451-459.
APA Zhang, Guiqiang,Jia, Peiyuan,Liu, Hongtao,Hu, Tao,&Du, Yuguang.(2018).Conjugation of chitosan oligosaccharides via a carrier protein markedly improves immunogenicity of porcine circovirus vaccine.GLYCOCONJUGATE JOURNAL,35(5),451-459.
MLA Zhang, Guiqiang,et al."Conjugation of chitosan oligosaccharides via a carrier protein markedly improves immunogenicity of porcine circovirus vaccine".GLYCOCONJUGATE JOURNAL 35.5(2018):451-459.
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