CAS OpenIR
Conjugation of chitosan oligosaccharides enhances immune response to porcine circovirus vaccine by activating macrophages
Zhang, Guiqiang1,2,3; Jia, Peiyuan2,3; Liu, Hongtao2,3; Hu, Tao2,3; Du, Yuguang2,3
2018-11-01
Source PublicationIMMUNOBIOLOGY
ISSN0171-2985
Volume223Issue:11Pages:663-670
Abstract

Porcine circovirus type 2 (PCV2)-associated diseases have led to great economic losses to the pig industry. Our lab previously found that conjugation of chitosan oligosaccharides (COS) or via a carrier protein enhanced the immunogenicity of PCV2 vaccine against infectious pathogens. However, precise mechanisms and signal transduction pathways underlying the efficacy of COS conjugation remains poorly defined. In this study, to better understand the effects and mechanism of COS conjugates maintain the adjuvant potential in vivo, we investigated its augmentation of macrophage function, including cell activation, NO production, cytokine production and phagocytosis. Additionally, the role of Toll-like receptors (TLR) proteins in this process was also assessed. The results indicate that, as compared to the PCV and PCV/COS, conjugation of COS effectively enhanced the NO production, cytokines generation and phagocytosis activity of macrophages. Noticeably, the generation of NO and proinflammatory cytokines was closely related to the TLR2/4 signaling pathways, strongly suggesting that conjugation of COS regulates innate and adaptive immunity by activation of macrophages, resulting in immune enhancement. In summary, the present study provides a potential mechanism of COS conjugation as a novel adjuvant to improve immune responses against various diseases.

KeywordAdjuvant Chitosan Oligosaccharide Conjugation Porcine Circovirus Type 2 (Pcv2) Macrophages Toll-like Receptors
DOI10.1016/j.imbio.2018.07.012
Language英语
WOS KeywordDendritic Cells ; Porphyra-haitanensis ; Cross-presentation ; Type-2 Pcv2 ; Receptor 4 ; In-vivo ; Innate ; Oligochitosan ; Inflammasome ; Mechanisms
Funding ProjectNational Key Research and Development Program of China[2017YFD0502303] ; National Natural Science Fund, China[31500747] ; National Natural Science Fund, China[31570801] ; National Natural Science Fund, China[U160820020]
WOS Research AreaImmunology
WOS SubjectImmunology
Funding OrganizationNational Key Research and Development Program of China ; National Natural Science Fund, China
WOS IDWOS:000446807400008
PublisherELSEVIER GMBH, URBAN & FISCHER VERLAG
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/26323
Collection中国科学院过程工程研究所
Corresponding AuthorLiu, Hongtao; Hu, Tao; Du, Yuguang
Affiliation1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, Key Lab Biopharmaceut Prod & Formulat Engn, PLA, Beijing 100190, Peoples R China
3.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Guiqiang,Jia, Peiyuan,Liu, Hongtao,et al. Conjugation of chitosan oligosaccharides enhances immune response to porcine circovirus vaccine by activating macrophages[J]. IMMUNOBIOLOGY,2018,223(11):663-670.
APA Zhang, Guiqiang,Jia, Peiyuan,Liu, Hongtao,Hu, Tao,&Du, Yuguang.(2018).Conjugation of chitosan oligosaccharides enhances immune response to porcine circovirus vaccine by activating macrophages.IMMUNOBIOLOGY,223(11),663-670.
MLA Zhang, Guiqiang,et al."Conjugation of chitosan oligosaccharides enhances immune response to porcine circovirus vaccine by activating macrophages".IMMUNOBIOLOGY 223.11(2018):663-670.
Files in This Item:
File Name/Size DocType Version Access License
Conjugation of chito(2482KB)期刊论文出版稿限制开放CC BY-NC-SAApplication Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zhang, Guiqiang]'s Articles
[Jia, Peiyuan]'s Articles
[Liu, Hongtao]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zhang, Guiqiang]'s Articles
[Jia, Peiyuan]'s Articles
[Liu, Hongtao]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zhang, Guiqiang]'s Articles
[Jia, Peiyuan]'s Articles
[Liu, Hongtao]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.