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Design and Biophysical Characterization of Poly (L-Lactic) Acid Microcarriers with and without Modification of Chitosan and Nanohydroxyapatite
Li, Liying1; Song, Kedong1; Chen, Yongzhi1; Wang, Yiwei2; Shi, Fangxin3; Nie, Yi4; Liu, Tianqing1
2018-10-01
Source PublicationPOLYMERS
ISSN2073-4360
Volume10Issue:10Pages:17
Abstract

Nowadays, microcarriers are widely utilized in drug delivery, defect filling, and cell culture. Also, many researchers focus on the combination of synthetic and natural polymers and bioactive ceramics to prepare composite biomaterials for tissue engineering and regeneration. In this study, three kinds of microcarriers were prepared based on physical doping and surface modification, named Poly (L-lactic) acid (PLLA), PLLA/nanohydroxyapatite (PLLA/nHA), and PLLA/nHA/Chitosan (PLLA/nHA/Ch). The physicochemical properties of the microcarriers and their functional performances in MC3T3-E1 cell culture were compared. Statistical results showed that the average diameter of PLLA microcarriers was 291.9 +/- 30.7 mu m, and that of PLLA/nHA and PLLA/nHA/Ch microcarriers decreased to 275.7 +/- 30.6 mu m and 269.4 +/- 26.3 mu m, respectively. The surface roughness and protein adsorption of microcarriers were enhanced with the doping of nHA and coating of chitosan. The cell-carrier cultivation stated that the PLLA/nHA microcarriers had the greatest proliferation-promoting effect, while the PLLA/nHA/Ch microcarriers performed the strongest attachment with MC3T3-E1 cells. Besides, the cells on the PLLA/nHA/Ch microcarriers exhibited optimal osteogenic expression. Generally, chitosan was found to improve microcarriers with superior characteristics in cell adhesion and differentiation, and nanohydroxyapatite was beneficial for microcarriers regarding sphericity and cell proliferation. Overall, the modified microcarriers may be considered as a promising tool for bone tissue engineering.

KeywordMicrocarriers Poly (L-lactic) Acid Chitosan Nanohydroxyapatite Osteoblasts
DOI10.3390/polym10101061
Language英语
WOS KeywordEmulsion-solvent Evaporation ; Mesenchymal Stem-cells ; Surface-roughness ; Plga Microspheres ; Bone Regeneration ; Drug-release ; Hydroxyapatite ; Plla ; Fabrication ; Composite
Funding ProjectNational Natural Science Foundation of China[31670978] ; National Natural Science Foundation of China[31370991] ; National Natural Science Foundation of China[21676041] ; Fok Ying Tung Education Foundation[132027] ; State Key Laboratory of Fine Chemicals[KF1111] ; Natural Science Foundation of Liaoning[20180510028] ; Fundamental Research Funds for the Central Universities[2016ZD210] ; SRF for ROCS, SEM[42]
WOS Research AreaPolymer Science
WOS SubjectPolymer Science
Funding OrganizationNational Natural Science Foundation of China ; Fok Ying Tung Education Foundation ; State Key Laboratory of Fine Chemicals ; Natural Science Foundation of Liaoning ; Fundamental Research Funds for the Central Universities ; SRF for ROCS, SEM
WOS IDWOS:000448662400016
PublisherMDPI
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/26427
Collection中国科学院过程工程研究所
Corresponding AuthorSong, Kedong; Nie, Yi; Liu, Tianqing
Affiliation1.Dalian Univ Technol, State Key Lab Fine Chem, Dalian R&D Ctr Stem Cell & Tissue Engn, Dalian 116024, Peoples R China
2.Univ Sydney, Burns Res Grp, ANZAC Res Inst, Sydney, NSW 2139, Australia
3.Zhengzhou Inst Emerging Technol Ind, Zhengzhou 450000, Henan, Peoples R China
4.Chinese Acad Sci, Key Lab Green Proc & Engn, Inst Proc Engn, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Li, Liying,Song, Kedong,Chen, Yongzhi,et al. Design and Biophysical Characterization of Poly (L-Lactic) Acid Microcarriers with and without Modification of Chitosan and Nanohydroxyapatite[J]. POLYMERS,2018,10(10):17.
APA Li, Liying.,Song, Kedong.,Chen, Yongzhi.,Wang, Yiwei.,Shi, Fangxin.,...&Liu, Tianqing.(2018).Design and Biophysical Characterization of Poly (L-Lactic) Acid Microcarriers with and without Modification of Chitosan and Nanohydroxyapatite.POLYMERS,10(10),17.
MLA Li, Liying,et al."Design and Biophysical Characterization of Poly (L-Lactic) Acid Microcarriers with and without Modification of Chitosan and Nanohydroxyapatite".POLYMERS 10.10(2018):17.
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