CAS OpenIR
Inhibitory effects of ammonia on syntrophic propionate oxidation in anaerobic digester sludge
Zhang, C; Yuan, Q; Lu, YH; Zhang, Chen; Yuan, Quan; Lu, Yahai
2018
Source PublicationWATER RESEARCH
ISSN0043-1354
Volume146Pages:275
Abstract

Syntrophic propionate oxidation (SPO) coupled with methanogenesis is often inhibited under high ammonium concentrations in anaerobic digesters. However, the inhibitory mechanism remains poorly understood. We conducted two independent laboratory experiments with a swine manure digester sludge. In experiment I, RNA-based stable isotope probing (SIP) was applied to determine the active players of both bacteria and methanogens involved in SPO under different ammonium concentrations (0, 3 and 7 g NE4+-N L-1). In experiment II, the dynamics of the bacterial community under ammonia stress was monitored using the 16S rRNA pyrosequencing and quantitative PCR under similar conditions as in experiment I but without the addition of external propionate. An additional higher ammonium treatment (10 g L-1) was applied in experiment II to maximize the ammonia stress. We identified that the Smithella bacteria and the Methanosaetaceae and Methanospirillaceae archaea were the most active players involved in SPO and methanogenesis. We revealed that Smithella, Methanosaetaceae and Methanospirillaceae were moderately and severely inhibited at 3 and 7-10 g NH4+-N L-1, respectively. However, the fermentative bacteria appeared to be more tolerant to ammonia stress. The microbial responses were corroborated with the accumulation of VFAs and the repression of methanogenesis under high ammonium conditions. (C) 2018 Elsevier Ltd. All rights reserved.

KeywordSyntrophic Propionate Oxidation Gen. Nov. Methanogenesis Smithella-propionica Ammonia Stress Oxidizing Bacteria Anaerobic Digester Sp. Nov. Stable Isotope Probe Community Acetate Degradation Hydrogen Fatty Acid
SubtypeArticle
DOI10.1016/j.watres.2018.09.046
Language英语
WOS IDWOS:000452565400025
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/26806
Collection中国科学院过程工程研究所
Recommended Citation
GB/T 7714
Zhang, C,Yuan, Q,Lu, YH,et al. Inhibitory effects of ammonia on syntrophic propionate oxidation in anaerobic digester sludge[J]. WATER RESEARCH,2018,146:275.
APA Zhang, C,Yuan, Q,Lu, YH,Zhang, Chen,Yuan, Quan,&Lu, Yahai.(2018).Inhibitory effects of ammonia on syntrophic propionate oxidation in anaerobic digester sludge.WATER RESEARCH,146,275.
MLA Zhang, C,et al."Inhibitory effects of ammonia on syntrophic propionate oxidation in anaerobic digester sludge".WATER RESEARCH 146(2018):275.
Files in This Item:
File Name/Size DocType Version Access License
Inhibitory effects o(3652KB)期刊论文出版稿限制开放CC BY-NC-SAApplication Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zhang, C]'s Articles
[Yuan, Q]'s Articles
[Lu, YH]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zhang, C]'s Articles
[Yuan, Q]'s Articles
[Lu, YH]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zhang, C]'s Articles
[Yuan, Q]'s Articles
[Lu, YH]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.