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A systematic mass-transfer modeling approach for mammalian cell culture bioreactor scale-up
He, Chuan1,2,4; Ye, Pei2; Wang, Haibin3; Liu, Xiao2; Li, Feng1,2
2019-01-15
Source PublicationBIOCHEMICAL ENGINEERING JOURNAL
ISSN1369-703X
Volume141Pages:173-181
Abstract

A mathematical mass-transfer model was developed based on the mass balance of gas exchange in cell culture bioreactors. The model combines the bioreactor mass transfer properties and mammalian cell metabolic parameters to predict the cell culture process oxygen demands and carbon dioxide accumulation in bioreactors. With the guidance of the mass-transfer model, a CHO cell culture process to produce a therapeutic antibody was successfully scaled up from 2-L to 1500-L bioreactor directly without intermediate steps. The process performances and product quality attributes demonstrate good robustness and consistency between small and large scales. This mass-transfer model provides a more systematic and reliable approach for mammalian cell culture bioreactor scaling up.

KeywordMammalian Cell Culture Mass-transfer Model Bioreactor Scale Up
DOI10.1016/j.bej.2018.09.019
Language英语
WOS KeywordDissolved-oxygen ; Elevated Pco(2) ; Growth ; Bicarbonate ; Osmolality ; Design ; Damage
WOS Research AreaBiotechnology & Applied Microbiology ; Engineering
WOS SubjectBiotechnology & Applied Microbiology ; Engineering, Chemical
WOS IDWOS:000453623900018
PublisherELSEVIER SCIENCE BV
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/27630
Collection中国科学院过程工程研究所
Corresponding AuthorLi, Feng
Affiliation1.Chinese Acad Sci, Inst Proc Engn, Beijing 100190, Peoples R China
2.Beijing Mabworks Biotech Co Ltd, Beijing 100176, Peoples R China
3.Zhejiang Hisun Pharmaceut Co Ltd, Hangzhou 311404, Zhejiang, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
He, Chuan,Ye, Pei,Wang, Haibin,et al. A systematic mass-transfer modeling approach for mammalian cell culture bioreactor scale-up[J]. BIOCHEMICAL ENGINEERING JOURNAL,2019,141:173-181.
APA He, Chuan,Ye, Pei,Wang, Haibin,Liu, Xiao,&Li, Feng.(2019).A systematic mass-transfer modeling approach for mammalian cell culture bioreactor scale-up.BIOCHEMICAL ENGINEERING JOURNAL,141,173-181.
MLA He, Chuan,et al."A systematic mass-transfer modeling approach for mammalian cell culture bioreactor scale-up".BIOCHEMICAL ENGINEERING JOURNAL 141(2019):173-181.
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