CAS OpenIR
Gas-assisted low-field magnetic separation for large scale continuous magnetic bio-separation process
Li, Wensong1; Yang, Liangrong2; Dong, Tingting2; Xing, Huifang2; Wang, Weiyan1; Yang, Yunquan1; Liu, Huizhou2
2019
Source PublicationAICHE JOURNAL
ISSN0001-1541
Volume65Issue:1Pages:175-183
AbstractIn this study, the gas-assisted low-field magnetic separation (GAMS) was proposed for continuous large-scale magnetic bio-separation process. Magnetic recovery processes in selective separation of binary mixed proteins by magnetic microspheres were selected as a model. Basing on magnetic recovery efficiency of the batch GAMS for this model, the continuous GAMS with an amplifying small pilot scale was further researched. The continuous throughput of GAMS for adsorption, washing, and desorption step reached 12 L/h, 12 L/h, and 18 L/h with an adsorbent recovery above 95%, respectively. Furthermore, magnetic particles via the top magnetic roller could be well-separated from free proteins with good floatability via the bottom outlet of flotation column, and bubbling operation did not cause the significant denaturation of target protein. Compared with classic high gradient magnetic separation, GAMS had the advantages of easy scale-up, easy continuous operation, and low-cost, showing great potential for large-scale magnetic bio-separation process. (c) 2018 American Institute of Chemical Engineers AIChE J, 65: 175-183, 2019
Keywordgas-assisted magnetic separation large-scale bio-separation continuous low field separation of proteins
DOI10.1002/aic.16389
Language英语
WOS KeywordIRON-OXIDE NANOPARTICLES ; SUPERPARAMAGNETIC EXTRACTION ; PURIFICATION ; FLOTATION
Funding ProjectNational Natural Science Foundation of China[21506180] ; National Natural Science Foundation of China[21676273] ; National Key Natural Science Foundation of China[U1507203] ; National Technology Research and Development Program of China[2015CB251402] ; Science and technology innovation project of CMST[17-163-12-ZD-001-013-01] ; Youth Innovation Promotion Association, CAS[2016043] ; Natural Science Foundation of Hunan Province, China[2016JJ3111]
WOS Research AreaEngineering
WOS SubjectEngineering, Chemical
Funding OrganizationNational Natural Science Foundation of China ; National Key Natural Science Foundation of China ; National Technology Research and Development Program of China ; Science and technology innovation project of CMST ; Youth Innovation Promotion Association, CAS ; Natural Science Foundation of Hunan Province, China
WOS IDWOS:000454632100017
PublisherWILEY
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/27675
Collection中国科学院过程工程研究所
Corresponding AuthorYang, Liangrong; Liu, Huizhou
Affiliation1.Xiangtan Univ, Coll Chem Engn, Xiangtan, Hunan, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, CAS Key Lab Green Proc & Engn, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Li, Wensong,Yang, Liangrong,Dong, Tingting,et al. Gas-assisted low-field magnetic separation for large scale continuous magnetic bio-separation process[J]. AICHE JOURNAL,2019,65(1):175-183.
APA Li, Wensong.,Yang, Liangrong.,Dong, Tingting.,Xing, Huifang.,Wang, Weiyan.,...&Liu, Huizhou.(2019).Gas-assisted low-field magnetic separation for large scale continuous magnetic bio-separation process.AICHE JOURNAL,65(1),175-183.
MLA Li, Wensong,et al."Gas-assisted low-field magnetic separation for large scale continuous magnetic bio-separation process".AICHE JOURNAL 65.1(2019):175-183.
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