CAS OpenIR
A new method of synthesis well-dispersion and dense Fe3O4@SiO2 magnetic nanoparticles for DNA extraction
Fan, Qingqian1; Guan, Yueping1; Zhang, Zhi1; Xu, Guoli1; Yang, Yu3; Guo, Chen2
2019
Source PublicationCHEMICAL PHYSICS LETTERS
ISSN0009-2614
Volume715Pages:7-13
Abstract

A new method was developed for preparing monodisperse Fe3O4@SiO2 magnetic core-shell nanoparticles via three steps. The obtained nanoparticles exhibit high saturation magnetization, good surface topography and dispersion as characterized by FTIR, SEM and VSM. The experimental results of hydrochloric acid treatment show that the obtained nanoparticles have excellent acid resistance and stable and compact silicon coating. The magnetic core-shell nanoparticles were further tested for purification of plasmid DNA from E. coli DH5a cells. The result of purification leaded to better adsorption, elution and purity results in comparison with isolated commercial extraction kit.

KeywordMagnetic Nanoparticles Core-shell Dna Extraction Fe3o4@sio2
DOI10.1016/j.cplett.2018.11.001
Language英语
WOS KeywordGenomic Dna ; Silica
Funding ProjectNational Key Research and Development Program of China[2018YFF0214900]
WOS Research AreaChemistry ; Physics
WOS SubjectChemistry, Physical ; Physics, Atomic, Molecular & Chemical
Funding OrganizationNational Key Research and Development Program of China
WOS IDWOS:000455097200002
PublisherELSEVIER SCIENCE BV
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/27714
Collection中国科学院过程工程研究所
Corresponding AuthorGuan, Yueping
Affiliation1.Univ Sci & Technol Beijing, Sch Mat Sci & Engn, Beijing 100083, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, Key Lab Green Proc & Engn, Beijing 100190, Peoples R China
3.Chinese Acad Inspect & Quarantine, Beijing 100176, Peoples R China
Recommended Citation
GB/T 7714
Fan, Qingqian,Guan, Yueping,Zhang, Zhi,et al. A new method of synthesis well-dispersion and dense Fe3O4@SiO2 magnetic nanoparticles for DNA extraction[J]. CHEMICAL PHYSICS LETTERS,2019,715:7-13.
APA Fan, Qingqian,Guan, Yueping,Zhang, Zhi,Xu, Guoli,Yang, Yu,&Guo, Chen.(2019).A new method of synthesis well-dispersion and dense Fe3O4@SiO2 magnetic nanoparticles for DNA extraction.CHEMICAL PHYSICS LETTERS,715,7-13.
MLA Fan, Qingqian,et al."A new method of synthesis well-dispersion and dense Fe3O4@SiO2 magnetic nanoparticles for DNA extraction".CHEMICAL PHYSICS LETTERS 715(2019):7-13.
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