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Synthesis and Evaluation of a Chitosan Oligosaccharide-Streptomycin Conjugate against Pseudomonas aeruginosa Biofilms
Li, Ruilian1,2; Yuan, Xianghua3; Wei, Jinhua2; Zhang, Xiafei3; Cheng, Gong2; Wang, Zhuo A.2; Du, Yuguang2
2019
Source PublicationMARINE DRUGS
ISSN1660-3397
Volume17Issue:1Pages:12
AbstractMicrobial biofilms are considerably more resistant to antibiotics than planktonic cells. It has been reported that chitosan coupling with the aminoglycoside antibiotic streptomycin dramatically disrupted biofilms of several Gram-positive bacteria. This finding suggested the application of the covalent conjugate of antimicrobial natural polysaccharides and antibiotics on anti-infection therapy. However, the underlying molecular mechanism of the chitosan-streptomycin conjugate (CS-Strep) remains unclear and the poor water-solubility of the conjugate might restrict its applications for anti-infection therapy. In this study, we conjugated streptomycin with water-soluble chitosan oligosaccharides (COS). Unlike CS-Strep, the COS-streptomycin conjugate (COS-Strep) barely affected biofilms of tested Gram-positive bacteria. However, COS-Strep efficiently eradicated established biofilms of the Gram-negative pathogen Pseudomonas aeruginosa. This activity of COS-Strep was influenced by the degree of polymerization of chitosan oligosaccharide. The increased susceptibility of P. aeruginosa biofilms to antibiotics after conjugating might be related to the following: Suppression of the activation of MexX-MexY drug efflux pump system induced by streptomycin treatment; and down-regulation of the biosynthesis of biofilm exopolysaccharides. Thus, this work indicated that covalently linking antibiotics to chitosan oligosaccharides was a possible approach for the development of antimicrobial drugs against biofilm-related infections.
Keywordchitosan oligosaccharides streptomycin Pseudomonas aeruginosa biofilms conjugation
DOI10.3390/md17010043
Language英语
WOS KeywordANTIBACTERIAL ACTIVITY ; ANTIBIOTIC-RESISTANCE ; PHARMACOKINETICS/PHARMACODYNAMICS ; COLISTIN ; IMIPENEM ; ASSAY
Funding ProjectNational Natural Science Fund, China[31670809] ; Key Research Program of the Chinese Academy of Sciences[KFZD-SW-218] ; Open Project Program of the Collaborative Innovation Center of Modern Bio-Manufacture, Anhui University[BM2016004]
WOS Research AreaPharmacology & Pharmacy
WOS SubjectChemistry, Medicinal
Funding OrganizationNational Natural Science Fund, China ; Key Research Program of the Chinese Academy of Sciences ; Open Project Program of the Collaborative Innovation Center of Modern Bio-Manufacture, Anhui University
WOS IDWOS:000458053200043
PublisherMDPI
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/27829
Collection中国科学院过程工程研究所
Corresponding AuthorWang, Zhuo A.; Du, Yuguang
Affiliation1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, PLA & State Key Lab Biochem Engn, Key Lab Biopharmaceut Prod & Formulat Engn, Beijing 100190, Peoples R China
3.Sichuan Normal Univ, Coll Life Sci, Chengdu 610101, Sichuan, Peoples R China
Recommended Citation
GB/T 7714
Li, Ruilian,Yuan, Xianghua,Wei, Jinhua,et al. Synthesis and Evaluation of a Chitosan Oligosaccharide-Streptomycin Conjugate against Pseudomonas aeruginosa Biofilms[J]. MARINE DRUGS,2019,17(1):12.
APA Li, Ruilian.,Yuan, Xianghua.,Wei, Jinhua.,Zhang, Xiafei.,Cheng, Gong.,...&Du, Yuguang.(2019).Synthesis and Evaluation of a Chitosan Oligosaccharide-Streptomycin Conjugate against Pseudomonas aeruginosa Biofilms.MARINE DRUGS,17(1),12.
MLA Li, Ruilian,et al."Synthesis and Evaluation of a Chitosan Oligosaccharide-Streptomycin Conjugate against Pseudomonas aeruginosa Biofilms".MARINE DRUGS 17.1(2019):12.
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