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An effective immobilized haloalkane dehalogenase DhaA from Rhodococcus rhodochrous by adsorption, crosslink and PEGylation on meso-cellular foam
Zheng, He1; Yu, Wei-Li2; Guo, Xuan1; Zhao, Yuan-Zhong1; Cui, Yan1; Hu, Tao2; Zhong, Jin-Yi1
2019-03-15
Source PublicationINTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
ISSN0141-8130
Volume125Pages:1016-1023
AbstractHaloalkane dehalogenase DhaA catalyzes the hydrolysis of halogenated compounds by cleavage of the carbon halogen bond. However, DhaA suffers from poor environmental stability and difficult recovery, which significantly increase the cost of DhaA. Here, an effective enzyme immobilization strategy was developed to overcome the disadvantages of DhaA. DhaA was physically absorbed with amine-functionalized meso-cellular foam (MCF). The MCF-absorbed DhaA (MD) was intermolecularly crosslinked with 8-arm PEG N-hydroxysuccinimide ester and then PEGylated by maleimide-thiol chemistry. DhaA from Rhodococcus rhodochrous was absorbed at a loading capacity of 100 mg/g in MD. The bulk crystallinity and morphology of MCF were largely maintained. The immobilized DhaA (MD-P1-P2) showed a lower Michaelis constant (K-m , 0.588 mM) than DhaA (0.905 mM), along with an extremely low leaching ratio of DhaA (1.1%) from MCF. MD-P1-P2 exhibited a high stability in the extreme environmental conditions, as reflected by the remaining activity of 99.8% in 40% (v/v) DMSO for 5 h, 87.3% in 3 M urea solution for 1 h, 25.9% at pH 3.0, and 51.8% at room temperature for 30 days. Thus, our study was expected to develop an effective immobilized DhaA for practical application. (C) 2018 Elsevier B.V. All rights reserved.
KeywordHaloalkane dehalogenase Meso-cellular foam Enzyme immobilization
DOI10.1016/j.ijbiomac.2018.12.127
Language英语
WOS KeywordENZYME IMMOBILIZATION ; MESOPOROUS MATERIALS ; MARKEDLY IMPROVES ; SURFACE ; HEXACHLOROCYCLOHEXANE ; STRATEGIES ; CRYSTALLIZATION ; BIODEGRADATION ; STABILIZATION ; STABILITY
Funding ProjectNational Natural Science Foundation of China[81703445] ; National Natural Science Foundation of China[81700181]
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry ; Polymer Science
WOS SubjectBiochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science
Funding OrganizationNational Natural Science Foundation of China
WOS IDWOS:000458222000113
PublisherELSEVIER SCIENCE BV
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/27850
Collection中国科学院过程工程研究所
Corresponding AuthorHu, Tao; Zhong, Jin-Yi
Affiliation1.Res Inst Chem Def, State Key Lab NBC Protect Civilian, Beijing 102205, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
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GB/T 7714
Zheng, He,Yu, Wei-Li,Guo, Xuan,et al. An effective immobilized haloalkane dehalogenase DhaA from Rhodococcus rhodochrous by adsorption, crosslink and PEGylation on meso-cellular foam[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2019,125:1016-1023.
APA Zheng, He.,Yu, Wei-Li.,Guo, Xuan.,Zhao, Yuan-Zhong.,Cui, Yan.,...&Zhong, Jin-Yi.(2019).An effective immobilized haloalkane dehalogenase DhaA from Rhodococcus rhodochrous by adsorption, crosslink and PEGylation on meso-cellular foam.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,125,1016-1023.
MLA Zheng, He,et al."An effective immobilized haloalkane dehalogenase DhaA from Rhodococcus rhodochrous by adsorption, crosslink and PEGylation on meso-cellular foam".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 125(2019):1016-1023.
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