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| Preparation of collagen/chitosan microspheres for 3D macrophage proliferation in vitro | |
| Wang, Di1,2; Wang, Meiyue2,3; Wang, Anhe2; Li, Jieling2; Li, Xin2,3; Jian, Honglei2; Bai, Shuo2; Yin, Jian1 | |
| 2019-07-05 | |
| Source Publication | COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
 |
| ISSN | 0927-7757 |
| Volume | 572Pages:266-273 |
| Abstract | Macrophages, as an important member of immune cells, have now become a research hotspot in the field of immunotherapy, which requires prominent proliferation in vitro for clinical use. Herein, we reported the fabrication of collagen/chitosan microspheres via emulsification for 3D macrophages proliferation. With glutaraldehyde (GA) as cross-linker, the microspheres have an average diameter of 200 mu m and positively charged surfaces, which are of great stability and biocompatibility. Compared with 2D cell proliferation, the 3D microspheres could dramatically enhance macrophages proliferation rate and promote their biological functions. Furthermore, macrophages on the surface of microspheres could be easily collected by blowing and suction, without the need of trypsin digesting or the degradation of microsphere. Hence the microspheres could be reused for macrophage proliferation repeatedly. This study highlights opportunities for development of high-effective cell proliferation approach and provides a great promise in immune cell based immunotherapy. |
| Keyword | Collagen Microspheres Emulsification Macrophage 3D proliferation |
| DOI | 10.1016/j.colsurfa.2019.04.007 |
| Language | 英语 |
| WOS Keyword | TUMOR-ASSOCIATED MACROPHAGES ; STEM-CELLS ; GROWTH ; MICROCARRIERS ; POLARIZATION ; COLLAGEN ; THERAPY |
| Funding Project | National Natural Science Foundation of China[21703253] ; National Natural Science Foundation of China[21774132] ; National Natural Science Foundation of China[21877052] ; National Natural Science Foundation of China[31700706] ; Natural Science Foundation for Distinguished Young Scholars of Jiangsu Province[BK20180030] ; Fundamental Research Funds for the Central Universities[JUSRP51712B] ; Max Planck Society International Partner Group Program |
| WOS Research Area | Chemistry |
| WOS Subject | Chemistry, Physical |
| Funding Organization | National Natural Science Foundation of China ; Natural Science Foundation for Distinguished Young Scholars of Jiangsu Province ; Fundamental Research Funds for the Central Universities ; Max Planck Society International Partner Group Program |
| WOS ID | WOS:000466612900029 |
| Publisher | ELSEVIER SCIENCE BV |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ipe.ac.cn/handle/122111/28146 |
| Collection | 中国科学院过程工程研究所 |
| Corresponding Author | Bai, Shuo; Yin, Jian |
| Affiliation | 1.Jiangnan Univ, Key Lab Carbohydrate Chem & Biotechnol, Minist Educ, Sch Biotechnol, Wuxi 214122, Peoples R China 2.Chinese Acad Sci, State Key Lab Biochem Engn, Inst Proc Engn, Beijing 100190, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| Recommended Citation GB/T 7714 | Wang, Di,Wang, Meiyue,Wang, Anhe,et al. Preparation of collagen/chitosan microspheres for 3D macrophage proliferation in vitro[J]. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,2019,572:266-273. |
| APA | Wang, Di.,Wang, Meiyue.,Wang, Anhe.,Li, Jieling.,Li, Xin.,...&Yin, Jian.(2019).Preparation of collagen/chitosan microspheres for 3D macrophage proliferation in vitro.COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,572,266-273. |
| MLA | Wang, Di,et al."Preparation of collagen/chitosan microspheres for 3D macrophage proliferation in vitro".COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS 572(2019):266-273. |
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