CAS OpenIR
Preparation of collagen/chitosan microspheres for 3D macrophage proliferation in vitro
Wang, Di1,2; Wang, Meiyue2,3; Wang, Anhe2; Li, Jieling2; Li, Xin2,3; Jian, Honglei2; Bai, Shuo2; Yin, Jian1
2019-07-05
Source PublicationCOLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
ISSN0927-7757
Volume572Pages:266-273
AbstractMacrophages, as an important member of immune cells, have now become a research hotspot in the field of immunotherapy, which requires prominent proliferation in vitro for clinical use. Herein, we reported the fabrication of collagen/chitosan microspheres via emulsification for 3D macrophages proliferation. With glutaraldehyde (GA) as cross-linker, the microspheres have an average diameter of 200 mu m and positively charged surfaces, which are of great stability and biocompatibility. Compared with 2D cell proliferation, the 3D microspheres could dramatically enhance macrophages proliferation rate and promote their biological functions. Furthermore, macrophages on the surface of microspheres could be easily collected by blowing and suction, without the need of trypsin digesting or the degradation of microsphere. Hence the microspheres could be reused for macrophage proliferation repeatedly. This study highlights opportunities for development of high-effective cell proliferation approach and provides a great promise in immune cell based immunotherapy.
KeywordCollagen Microspheres Emulsification Macrophage 3D proliferation
DOI10.1016/j.colsurfa.2019.04.007
Language英语
WOS KeywordTUMOR-ASSOCIATED MACROPHAGES ; STEM-CELLS ; GROWTH ; MICROCARRIERS ; POLARIZATION ; COLLAGEN ; THERAPY
Funding ProjectNational Natural Science Foundation of China[21703253] ; National Natural Science Foundation of China[21774132] ; National Natural Science Foundation of China[21877052] ; National Natural Science Foundation of China[31700706] ; Natural Science Foundation for Distinguished Young Scholars of Jiangsu Province[BK20180030] ; Fundamental Research Funds for the Central Universities[JUSRP51712B] ; Max Planck Society International Partner Group Program
WOS Research AreaChemistry
WOS SubjectChemistry, Physical
Funding OrganizationNational Natural Science Foundation of China ; Natural Science Foundation for Distinguished Young Scholars of Jiangsu Province ; Fundamental Research Funds for the Central Universities ; Max Planck Society International Partner Group Program
WOS IDWOS:000466612900029
PublisherELSEVIER SCIENCE BV
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/28146
Collection中国科学院过程工程研究所
Corresponding AuthorBai, Shuo; Yin, Jian
Affiliation1.Jiangnan Univ, Key Lab Carbohydrate Chem & Biotechnol, Minist Educ, Sch Biotechnol, Wuxi 214122, Peoples R China
2.Chinese Acad Sci, State Key Lab Biochem Engn, Inst Proc Engn, Beijing 100190, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Wang, Di,Wang, Meiyue,Wang, Anhe,et al. Preparation of collagen/chitosan microspheres for 3D macrophage proliferation in vitro[J]. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,2019,572:266-273.
APA Wang, Di.,Wang, Meiyue.,Wang, Anhe.,Li, Jieling.,Li, Xin.,...&Yin, Jian.(2019).Preparation of collagen/chitosan microspheres for 3D macrophage proliferation in vitro.COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,572,266-273.
MLA Wang, Di,et al."Preparation of collagen/chitosan microspheres for 3D macrophage proliferation in vitro".COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS 572(2019):266-273.
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