CAS OpenIR
Identification of a targeting-delivery peptide based on rhCNB
Yang, JJ; Gao, YD; Zhu, ZW; Qin, NN; Wei, Q; Yang, Jinju; Gao, Yadan; Zhu, Ziwei; Qin, Nannan; Wei, Qun
2019
Source PublicationJOURNAL OF PEPTIDE SCIENCE
ISSN1075-2617
Volume25Issue:6
AbstractCalcineurin B subunit (CNB) is the regulatory subunit of calcineurin (CN), and its classical function is to regulate the activity of CN. Research in our laboratory has revealed that the recombinant human CNB (rhCNB) is a good antitumor candidate and can be internalized by tumor cells via TLR4 receptor complexes and targeted to tumor tissue in nude mice. However, the fragment or domain of rhCNB mediating internalization and target delivery has not been identified. To explore fragment- mediated rhCNB internalization and target delivery, we generated truncated derivatives of rhCNBs by recombinant DNA technology and examined their cellular uptake. Interactions between truncated rhCNBs and the TLR4 receptor were studied by ELISA and co-immunoprecipitation, and targeting of model tumors in nude mice was examined. The results showed that one truncated derivative, Trun3 (124-169aa), was taken up by cells and targeted tumors with almost the same efficiency as intact rhCNB. These results indicate that Trun3 (45aa) contains the major sequence responsible for rhCNB internalization and tumor targeting and might be developed for drug delivery to tumors.
Keywordcellular uptake CALCINEURIN B-SUBUNIT targeting delivery INNATE IMMUNITY TLR4 PROTEIN truncated rhCNBs RECOGNITION ACTS
SubtypeArticle
DOI10.1002/psc.3159
WOS IDWOS:000469399800004
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/28166
Collection中国科学院过程工程研究所
Recommended Citation
GB/T 7714
Yang, JJ,Gao, YD,Zhu, ZW,et al. Identification of a targeting-delivery peptide based on rhCNB[J]. JOURNAL OF PEPTIDE SCIENCE,2019,25(6).
APA Yang, JJ.,Gao, YD.,Zhu, ZW.,Qin, NN.,Wei, Q.,...&Wei, Qun.(2019).Identification of a targeting-delivery peptide based on rhCNB.JOURNAL OF PEPTIDE SCIENCE,25(6).
MLA Yang, JJ,et al."Identification of a targeting-delivery peptide based on rhCNB".JOURNAL OF PEPTIDE SCIENCE 25.6(2019).
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