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A simple kinetic model for myeloma cell culture with consideration of lysine limitation
Alternative TitleBioprocess. Biosyst. Eng.
Liu, Yuan-Hua1; Bi, Jing-Xiu2,3; Zeng, An-Ping4,5; Yuan, Jing-Qi1
2008-10-01
Source PublicationBIOPROCESS AND BIOSYSTEMS ENGINEERING
ISSN1615-7591
Volume31Issue:6Pages:569-577
AbstractA simple kinetic model is developed to describe the dynamic behavior of myeloma cell growth and cell metabolism. Glucose, glutamine as well as lysine are considered as growth limiting substrates. The cell growth was restricted as soon as the extracellular lysine is exhausted and then intracellular lysine becomes a growth limiting substrate. In addition, a metabolic regulator model together with the Monod model is used to deal with the growth lag phase after inoculation or feeding. By using these models, concentrations of substrates and metabolites, as well as densities of viable and dead cells are quantitatively described. One batch cultivation and two fed-batch cultivations with pulse feeding of nutrients are used to validate the model.; A simple kinetic model is developed to describe the dynamic behavior of myeloma cell growth and cell metabolism. Glucose, glutamine as well as lysine are considered as growth limiting substrates. The cell growth was restricted as soon as the extracellular lysine is exhausted and then intracellular lysine becomes a growth limiting substrate. In addition, a metabolic regulator model together with the Monod model is used to deal with the growth lag phase after inoculation or feeding. By using these models, concentrations of substrates and metabolites, as well as densities of viable and dead cells are quantitatively described. One batch cultivation and two fed-batch cultivations with pulse feeding of nutrients are used to validate the model.
KeywordMyeloma Cell Line Kinetic Model Amino Acids Lysine Modeling Growth Kinetics
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Technology
DOI10.1007/s00449-008-0204-x
URL查看原文
Indexed BySCI
Language英语
WOS KeywordAMINO-ACID-CONCENTRATIONS ; MONOCLONAL-ANTIBODY PRODUCTION ; HYBRIDOMA CELLS ; MATHEMATICAL DESCRIPTIONS ; ENDOTHELIAL-CELLS ; BATCH CULTURE ; CHO-CELLS ; METABOLISM ; GROWTH ; SINGLE
WOS Research AreaBiotechnology & Applied Microbiology ; Engineering
WOS SubjectBiotechnology & Applied Microbiology ; Engineering, Chemical
WOS IDWOS:000258833600007
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Document Type期刊论文
Version出版稿
Identifierhttp://ir.ipe.ac.cn/handle/122111/2816
Collection研究所(批量导入)
Affiliation1.Shanghai Jiao Tong Univ, Dept Automat, Shanghai 200240, Peoples R China
2.Univ Adelaide, Sch Chem Engn, Adelaide, SA 5005, Australia
3.Chinese Acad Sci, Inst Proc Engn, Beijing 100080, Peoples R China
4.Tech Univ Hamburg, Hamburg Univ Technol, Inst Bioproc & Biosyst Engn, D-21073 Hamburg, Germany
5.Helmholtz Zentrum Infekt Forsch, Res Grp Syst Biol, D-38124 Braunschweig, Germany
Recommended Citation
GB/T 7714
Liu, Yuan-Hua,Bi, Jing-Xiu,Zeng, An-Ping,et al. A simple kinetic model for myeloma cell culture with consideration of lysine limitation[J]. BIOPROCESS AND BIOSYSTEMS ENGINEERING,2008,31(6):569-577.
APA Liu, Yuan-Hua,Bi, Jing-Xiu,Zeng, An-Ping,&Yuan, Jing-Qi.(2008).A simple kinetic model for myeloma cell culture with consideration of lysine limitation.BIOPROCESS AND BIOSYSTEMS ENGINEERING,31(6),569-577.
MLA Liu, Yuan-Hua,et al."A simple kinetic model for myeloma cell culture with consideration of lysine limitation".BIOPROCESS AND BIOSYSTEMS ENGINEERING 31.6(2008):569-577.
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