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Effects of concentration variation on the physical properties of alginate-based substrates and cell behavior in culture
Li, Liying1; Chen, Yongzhi1; Wang, Yiwei2; Shi, Fangxin3; Nie, Yi4,5; Liu, Tianqing1; Song, Kedong1
2019-05-01
Source PublicationINTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
ISSN0141-8130
Volume128Pages:184-195
AbstractNowadays alginate capsules exhibit good biocompatibility and high permeability for nutrients and metabolic wastes making them appealing biomaterial for therapeutic cell encapsulation. Further study of the characteristics of alginate beads which are highly dependent on various environmental conditions to create an optimum micro environment for cells is also critical. Thus, in this study, the effect of concentration variation on the physical properties of alginate-based beads and entrapped-cells behavior was analyzed. Results showed that the increase of Ca ions concentration brought about the decrease of the average diameter, prolongation of dissolution time, reduction of permeability and swelling, and a rise of crosslinking extent and shrinkage of capsules; while raising sodium alginate concentration had an opposite effect on the diameter and shrinkage. Moreover, the addition of gelatin enhanced the penetration and swelling and slowed down the shrinkage of capsules. And MC3T3-E1 cells enclosed in the particles in which the concentration of calcium chloride, sodium alginate and gelatin was 2.5%, 2.0% and 0.5% (w/v %) had preferable abilities of proliferation and higher expression of alkaline phosphatase. Overall, the ability to tailor this system to support in vitro growth of MC3T3-E1 cells might have significance for the future use of other cell types in regenerative medicine. (C) 2019 Published by Elsevier B.V.
KeywordCell encapsulation Alginate Gelatin
DOI10.1016/j.ijbiomac.2019.01.123
Language英语
WOS KeywordMESENCHYMAL STEM-CELLS ; MECHANICAL-PROPERTIES ; IN-VITRO ; HYDROGELS ; POLYMER ; BEADS ; DIFFERENTIATION ; ENCAPSULATION ; MICROBEADS ; EXPANSION
Funding ProjectNational Natural Science Foundation of China[31670978/31370991/21676041] ; Fok Ying Tung Education Foundation[132027] ; State Key Laboratory of Fine Chemicals[KF1111] ; Natural Science Foundation of Liaoning Province[20180510028]
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry ; Polymer Science
WOS SubjectBiochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science
Funding OrganizationNational Natural Science Foundation of China ; Fok Ying Tung Education Foundation ; State Key Laboratory of Fine Chemicals ; Natural Science Foundation of Liaoning Province
WOS IDWOS:000463305100024
PublisherELSEVIER SCIENCE BV
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/28220
Collection中国科学院过程工程研究所
Corresponding AuthorShi, Fangxin; Nie, Yi; Liu, Tianqing; Song, Kedong
Affiliation1.Dalian Univ Technol, State Key Lab Fine Chem, Dalian R&D Ctr Stem Cell & Tissue Engn, Dalian 116024, Peoples R China
2.Univ Sydney, ANZAC Res Inst, Burns Res Grp, Sydney, NSW 2139, Australia
3.Dalian Med Univ, Dept Oncol, Affiliated Hosp 1, Dalian 116011, Peoples R China
4.Zhengzhou Inst Emerging Technol Ind, Zhengzhou 450000, Peoples R China
5.Chinese Acad Sci, Inst Proc Engn, Key Lab Green Proc & Engn, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Li, Liying,Chen, Yongzhi,Wang, Yiwei,et al. Effects of concentration variation on the physical properties of alginate-based substrates and cell behavior in culture[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2019,128:184-195.
APA Li, Liying.,Chen, Yongzhi.,Wang, Yiwei.,Shi, Fangxin.,Nie, Yi.,...&Song, Kedong.(2019).Effects of concentration variation on the physical properties of alginate-based substrates and cell behavior in culture.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,128,184-195.
MLA Li, Liying,et al."Effects of concentration variation on the physical properties of alginate-based substrates and cell behavior in culture".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 128(2019):184-195.
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