CAS OpenIR
Rapid detection of severe fever with thrombocytopenia syndrome virus (SFTSV) total antibodies by up-converting phosphor technology-based lateral-flow assay
Huang, C; Wei, QZ; Hu, QS; Wen, T; Xue, L; Li, S; Zeng, XY; Shi, FJ; Jiao, YJ; Zhou, L; Huang, Chao; Wei, Qiaozhen; Hu, Qiushi; Wen, Tian; Xue, Lei; Li, Shuang; Zeng, Xiaoyan; Shi, Fengjuan; Jiao, Yongjun; Zhou, Lei
2019
Source PublicationLUMINESCENCE
ISSN1522-7235
Volume34Issue:2Pages:162
AbstractIn this study, an up-converting phosphor technology-based lateral-flow (UPT-LF) assay was developed to detect severe fever with thrombocytopenia syndrome virus (SFTSV) total antibodies rapidly and specifically. SFTSV recombinant N protein (SFTSV-rNP) was coated on analytical membrane for sample capture, up-converting phosphor (UCP) particles were used as the reporter, the luminescence emitted by UCP particles was converted to a measurable signal by a biosensor. The performance of UPT-LF assay was evaluated by testing 302 field serum samples by ELISA (enzyme-linked immunosorbent assay), Western blotting and UPT-LF assay. UPT-LF assay exhibited a lower detection limit than ELISA, and a satisfied level of agreement was exhibited by Kappa statistics (Kappa coefficient = 0.938). Considering Western blotting as the reference for comparison, the sensitivity and specificity of UPT-LF assay could reach 98.31% and 100%. UPT-LF assay showed no specific reaction with hantavirus total serum antibodies, which avoids the misdiagnosis of SFTSV from hantavirus that could cause similar clinical symptoms. UPT-LF assay was able to achieve acceptable results within 15 min and needed only 10 mu L sample for each test. As a whole, UPT-LF assay is a candidate method for on-site surveillance of SFTSV total antibodies owing to its excellent sensitivity, specificity, stability, easy operation and for being less time consuming.
Keywordrapid detection HEMORRHAGIC-FEVER severe fever with thrombocytopenia syndrome virus BUNYAVIRUS up-converting phosphor technology-based lateral-flow assay REPORTERS CHINA CELL
SubtypeArticle
DOI10.1002/bio.3588
WOS IDWOS:000460079900003
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/28328
Collection中国科学院过程工程研究所
Recommended Citation
GB/T 7714
Huang, C,Wei, QZ,Hu, QS,et al. Rapid detection of severe fever with thrombocytopenia syndrome virus (SFTSV) total antibodies by up-converting phosphor technology-based lateral-flow assay[J]. LUMINESCENCE,2019,34(2):162, 167.
APA Huang, C.,Wei, QZ.,Hu, QS.,Wen, T.,Xue, L.,...&Zhou, Lei.(2019).Rapid detection of severe fever with thrombocytopenia syndrome virus (SFTSV) total antibodies by up-converting phosphor technology-based lateral-flow assay.LUMINESCENCE,34(2),162.
MLA Huang, C,et al."Rapid detection of severe fever with thrombocytopenia syndrome virus (SFTSV) total antibodies by up-converting phosphor technology-based lateral-flow assay".LUMINESCENCE 34.2(2019):162.
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