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Improved blood compatibility of polysulfone membrane by anticoagulant protein immobilization
Ji, Mingbo1,2; Chen, Xiangrong1; Luo, Jianquan1,2; Wan, Yinhua1,2
2019-03-01
Source PublicationCOLLOIDS AND SURFACES B-BIOINTERFACES
ISSN0927-7765
Volume175Pages:586-595
AbstractTwo anticoagulant proteins, nattokinase and lumbrokinase, were successfully immobilized onto the dopamine coated polysulfone (PSf) membrane surface by covalent bonding. X-ray photoelectron spectroscopy (XPS) and Zeta potential measurement confirmed the immobilization of,these anticoagulant proteins. The immobilization yield of nattokinase and lumbrokinase reached to 35.2 and 33.4 mu g/cm(2), respectively. The water contact angle measurement revealed that the membrane surface hydrophilicity was improved after immobilizing the anticoagulant proteins. Meanwhile, the immobilized proteins retained their biological activity. Then blood compatible tests demonstrated that the modified membranes had lower static protein adsorption, platelet/erythrocyte adhesion, hemolysis, as well as longer blood clotting time, compared to virgin PSf membrane. In addition, the nattokinase-immobilized membrane showed a higher blood compatibility than BSA and lumbrokinase immobilized memrbanes, due to its' high bioactivity. Our research demonstrates that the dopamine-assisted immobilization of nattokinase and lumbrokinase can endow the membranes with improved blood compatibility as well as high biological activity, which may be expected to apply to blood-contacting materials.
KeywordDopamine Nattokinase Lumbrokinase Immobilization Enzymatic activity Blood compatibility
DOI10.1016/j.colsurfb.2018.12.026
Language英语
WOS KeywordBOVINE SERUM-ALBUMIN ; HOLLOW FIBERS ; CITRIC-ACID ; HEPARIN ; SURFACE ; HEMOCOMPATIBILITY ; BIOCOMPATIBILITY ; ANTIBACTERIAL ; COAGULATION ; ADSORPTION
Funding ProjectNational Nature Science Foundation of China[21104086] ; National High Technology Research and Development Program of China ("863" Program)[2014AA021005]
WOS Research AreaBiophysics ; Chemistry ; Materials Science
WOS SubjectBiophysics ; Chemistry, Physical ; Materials Science, Biomaterials
Funding OrganizationNational Nature Science Foundation of China ; National High Technology Research and Development Program of China ("863" Program)
WOS IDWOS:000459837800067
PublisherELSEVIER SCIENCE BV
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/28329
Collection中国科学院过程工程研究所
Corresponding AuthorChen, Xiangrong; Wan, Yinhua
Affiliation1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Ji, Mingbo,Chen, Xiangrong,Luo, Jianquan,et al. Improved blood compatibility of polysulfone membrane by anticoagulant protein immobilization[J]. COLLOIDS AND SURFACES B-BIOINTERFACES,2019,175:586-595.
APA Ji, Mingbo,Chen, Xiangrong,Luo, Jianquan,&Wan, Yinhua.(2019).Improved blood compatibility of polysulfone membrane by anticoagulant protein immobilization.COLLOIDS AND SURFACES B-BIOINTERFACES,175,586-595.
MLA Ji, Mingbo,et al."Improved blood compatibility of polysulfone membrane by anticoagulant protein immobilization".COLLOIDS AND SURFACES B-BIOINTERFACES 175(2019):586-595.
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