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| Improved blood compatibility of polysulfone membrane by anticoagulant protein immobilization | |
| Ji, Mingbo1,2; Chen, Xiangrong1; Luo, Jianquan1,2; Wan, Yinhua1,2 | |
| 2019-03-01 | |
| Source Publication | COLLOIDS AND SURFACES B-BIOINTERFACES
 |
| ISSN | 0927-7765 |
| Volume | 175Pages:586-595 |
| Abstract | Two anticoagulant proteins, nattokinase and lumbrokinase, were successfully immobilized onto the dopamine coated polysulfone (PSf) membrane surface by covalent bonding. X-ray photoelectron spectroscopy (XPS) and Zeta potential measurement confirmed the immobilization of,these anticoagulant proteins. The immobilization yield of nattokinase and lumbrokinase reached to 35.2 and 33.4 mu g/cm(2), respectively. The water contact angle measurement revealed that the membrane surface hydrophilicity was improved after immobilizing the anticoagulant proteins. Meanwhile, the immobilized proteins retained their biological activity. Then blood compatible tests demonstrated that the modified membranes had lower static protein adsorption, platelet/erythrocyte adhesion, hemolysis, as well as longer blood clotting time, compared to virgin PSf membrane. In addition, the nattokinase-immobilized membrane showed a higher blood compatibility than BSA and lumbrokinase immobilized memrbanes, due to its' high bioactivity. Our research demonstrates that the dopamine-assisted immobilization of nattokinase and lumbrokinase can endow the membranes with improved blood compatibility as well as high biological activity, which may be expected to apply to blood-contacting materials. |
| Keyword | Dopamine Nattokinase Lumbrokinase Immobilization Enzymatic activity Blood compatibility |
| DOI | 10.1016/j.colsurfb.2018.12.026 |
| Language | 英语 |
| WOS Keyword | BOVINE SERUM-ALBUMIN ; HOLLOW FIBERS ; CITRIC-ACID ; HEPARIN ; SURFACE ; HEMOCOMPATIBILITY ; BIOCOMPATIBILITY ; ANTIBACTERIAL ; COAGULATION ; ADSORPTION |
| Funding Project | National Nature Science Foundation of China[21104086] ; National High Technology Research and Development Program of China ("863" Program)[2014AA021005] |
| WOS Research Area | Biophysics ; Chemistry ; Materials Science |
| WOS Subject | Biophysics ; Chemistry, Physical ; Materials Science, Biomaterials |
| Funding Organization | National Nature Science Foundation of China ; National High Technology Research and Development Program of China ("863" Program) |
| WOS ID | WOS:000459837800067 |
| Publisher | ELSEVIER SCIENCE BV |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ipe.ac.cn/handle/122111/28329 |
| Collection | 中国科学院过程工程研究所 |
| Corresponding Author | Chen, Xiangrong; Wan, Yinhua |
| Affiliation | 1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| Recommended Citation GB/T 7714 | Ji, Mingbo,Chen, Xiangrong,Luo, Jianquan,et al. Improved blood compatibility of polysulfone membrane by anticoagulant protein immobilization[J]. COLLOIDS AND SURFACES B-BIOINTERFACES,2019,175:586-595. |
| APA | Ji, Mingbo,Chen, Xiangrong,Luo, Jianquan,&Wan, Yinhua.(2019).Improved blood compatibility of polysulfone membrane by anticoagulant protein immobilization.COLLOIDS AND SURFACES B-BIOINTERFACES,175,586-595. |
| MLA | Ji, Mingbo,et al."Improved blood compatibility of polysulfone membrane by anticoagulant protein immobilization".COLLOIDS AND SURFACES B-BIOINTERFACES 175(2019):586-595. |
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