CAS OpenIR
Unique stabilizing mechanism provided by biocompatible choline-based ionic liquids for inhibiting dissociation of inactivated foot-and-mouth disease virus particles
Lin, Xuan1,2; Yang, Yanli1; Li, Shuai1,2; Song, Yanmin1,2; Ma, Guanghui1; Su, Zhiguo1; Zhang, Songping1
2019
Source PublicationRSC ADVANCES
ISSN2046-2069
Volume9Issue:24Pages:13933-13939
AbstractInactivated virus and virus-like particles (VLPs) are important classes of biopharmaceuticals for vaccines, immunotherapy and oncotherapy. Their complex particle structures are easily denatured during processing and storage, leading to loss in their biofunctionality. Ionic liquids (ILs) as stabilizing excipients have garnered interest in protein-based pharmaceutical research, but their stabilizing capacity for inactivated virus antigens remains unknown. Here, three biocompatible choline-based ILs, including [Cho][H2PO4], [Cho][Cl], and [Cho][SO4], were tested as potential stabilizers for the inactivated foot-and-mouth disease virus (iFMDV), which are extremely unstable virus particles easily dissociating into smaller pentamers named 12S. Based on differential scanning fluorimetry technology for thermal stability analysis, together with high-performance size-exclusion chromatography for quantitative determination of 146S, it was found that [Cho][Cl] and [cho][SO4] can improve the thermo- and long-term storage stability of iFMDV particles, while [Cho][H2PO4] showed a destabilizing effect. Animal experiments indicated that the immunogenicity of iFMDV antigens was not attenuated in all three ILs. By monitoring the microenvironmental pH of the virus particles in different ILs, a relatively lower proton intensity was observed in [Cho][Cl] and [Cho][SO4] than in buffers and [Cho][H2PO4]. Therefore, the stabilizing mechanism was supposed to be mainly due to suppression of protonation of histidine residues in the inter-pentamer interface of virus particles in [Cho][Cl] and [Cho][SO4], which is distinct from the mechanism reported for other proteins with relatively simple structures. The results suggest that the choline-based ILs with appropriate anions are promising stabilizing excipients for iFMDV or other vaccine antigens.
DOI10.1039/c9ra02722j
Language英语
WOS KeywordSIZE-EXCLUSION HPLC ; VACCINE
Funding ProjectNational Natural Sciences Foundation of China[21808226] ; National Natural Sciences Foundation of China[21821005]
WOS Research AreaChemistry
WOS SubjectChemistry, Multidisciplinary
Funding OrganizationNational Natural Sciences Foundation of China
WOS IDWOS:000468639300063
PublisherROYAL SOC CHEMISTRY
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/28358
Collection中国科学院过程工程研究所
Corresponding AuthorSu, Zhiguo; Zhang, Songping
Affiliation1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Lin, Xuan,Yang, Yanli,Li, Shuai,et al. Unique stabilizing mechanism provided by biocompatible choline-based ionic liquids for inhibiting dissociation of inactivated foot-and-mouth disease virus particles[J]. RSC ADVANCES,2019,9(24):13933-13939.
APA Lin, Xuan.,Yang, Yanli.,Li, Shuai.,Song, Yanmin.,Ma, Guanghui.,...&Zhang, Songping.(2019).Unique stabilizing mechanism provided by biocompatible choline-based ionic liquids for inhibiting dissociation of inactivated foot-and-mouth disease virus particles.RSC ADVANCES,9(24),13933-13939.
MLA Lin, Xuan,et al."Unique stabilizing mechanism provided by biocompatible choline-based ionic liquids for inhibiting dissociation of inactivated foot-and-mouth disease virus particles".RSC ADVANCES 9.24(2019):13933-13939.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Lin, Xuan]'s Articles
[Yang, Yanli]'s Articles
[Li, Shuai]'s Articles
Baidu academic
Similar articles in Baidu academic
[Lin, Xuan]'s Articles
[Yang, Yanli]'s Articles
[Li, Shuai]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Lin, Xuan]'s Articles
[Yang, Yanli]'s Articles
[Li, Shuai]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.