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人血清转铁蛋白标准物质纯化
孔金隆; 全灿; 王启迪; 罗坚; 周俊波; 孙永跃; 李增兰
2017
Source Publication过程工程学报
Volume18Issue:02Pages:387-392
Abstract

建立了一种从Cohn血浆分离副产物组分IV中纯化高纯度转铁蛋白(Tf)标准物质的技术,对Cohn组分IV进行复溶、离心和过滤,采用Capto DEAE阴离子交换色谱进行初分离,再通过穿透式Octyl-Sepharose 4 Fast Flow疏水色谱进一步精制;对纯化产品进行了结构、纯度、活性等表征,采用十二烷基磺酸钠-聚丙烯酰胺凝胶电泳、基质辅助激光解吸电离飞行时间质谱、高效液相色谱及酶联免疫吸附法对Tf标准物质进行了分析.结果表明,Tf产品纯度大于99%,收率为79%;圆二色光谱分析显示Tf的二级结构未发生改变,铁离子结合能力实验表明Tf可结合2个铁离子,保持活性.

Keyword人血清转铁蛋白 标准物质 离子交换色谱 疏水色谱 Cohn组分ⅳ 纯化
Language中文
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/28759
Collection中国科学院过程工程研究所
Recommended Citation
GB/T 7714
孔金隆,全灿,王启迪,等. 人血清转铁蛋白标准物质纯化[J]. 过程工程学报,2017,18(02):387-392.
APA 孔金隆.,全灿.,王启迪.,罗坚.,周俊波.,...&李增兰.(2017).人血清转铁蛋白标准物质纯化.过程工程学报,18(02),387-392.
MLA 孔金隆,et al."人血清转铁蛋白标准物质纯化".过程工程学报 18.02(2017):387-392.
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