pH-responsive Poly(D,L-Lactic-co-Glycolic Acid) Nanoparticles with Rapid Antigen Release Behavior Promote Immune Response
Wang LY(王连艳)
Conference Name第十三届全国免疫学学术大会
Source Publication第十三届全国免疫学学术大会摘要汇编
Conference Date2018-11-07
Conference Place中国上海

We fabricated pH-responsive PLGA NPs with rapid antigen intracellular release behavior in APCs.The NPs, which had thin shells and large inner space, contain ammonium bicarbonate(NH_4HCO_3) that could regulate release in endosomes and lysosomes acting as antigen release promoter in DCs was co-encapsulated with antigen(OVA).Hydrogen ions(H~+) in DC endosomes and lysosomes(pH ~5.0 and 6.5) could react with NH_4HCO_3 to generate NH_3 and CO_2, which broke NPs and released antigens.Then, the immune responses in bone marrow-derived dendritic cells(BMDCs) in vitro and in mice were examined.Antigens encapsulated in pH-responsive PLGA NPs could escape from lysosomes into the cytoplasm and be cross-presented.Mouse immunization with pH-responsive PLGA NPs induced greater lymphocyte activation, more antigen-specific CD8~+ T cells, stronger cytotoxic capacity(IFN-γ and granzyme B), enhanced antigen-specific IgG antibodies and higher serum IgG2a/IgG1.Thus, pH-responsive PLGA NPs could be potentially useful as effective vaccine delivery and adjuvant systems for the therapy of intracellular infectious diseases and virus infection.

KeywordAntigen Intracellular Rapid Release Dendritic Cells Ph-responsive Plga Nanoparticles Vaccine Del
Document Type会议论文
Recommended Citation
GB/T 7714
Wang LY. pH-responsive Poly(D,L-Lactic-co-Glycolic Acid) Nanoparticles with Rapid Antigen Release Behavior Promote Immune Response[C],2018.
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