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A new washing-free immunosensor for tumor marker detection based on functionalized Fe3O4 submicron particles
Zhang, Zhi1; Guan, Yueping1; Xu, Guoli1; Guo, Chen2
2019-06-01
Source PublicationMICROCHEMICAL JOURNAL
ISSN0026-265X
Volume147Pages:824-831
Abstract

In this submission, a novel washing-free immunosensor was designed for chemiluminescence immunoassay (CLIA) of tumor marker using human Alpha-fetoprotein (AFP) as a representative. Reactive groups were introduced on the surface of the solvothermally prepared Fe3O4 magnetic submicron particles (MSPs) and the dual-functional polyethylene glycol (PEG) linkers were modified to reduce the non-specific adsorption capacity of the particle surface and further to immobilize N-(4-aminohexyl)-N-ethylisoluminol (AHEI) and anti-AFP antibody on the surface of the particles. After incubation with AFP and horseradish peroxidase (HRP) labeled anti-AFP antibody respectively, the formed sandwich-type immunocomplexes on the particle surface could lead to the close proximity between AHEI and HRP, thus eventually leads to an increase in the detected chemiluminescence (CL) signal. As a bound HRP labeled anti-AFP antibody produces much stronger CL signal than an unbound one, this approach eliminates the time- and labor-consuming washing steps usually required of such assays. Under the optimal conditions, this present method shows a wide quantitative range from 1 to 250 ng mL(-1) with a detection limit of 1.751 ng mL(-1). The detection limit was sufficient for measuring physiological (0-20 ng mL(-1)) and clinical ( > 20 ng mL(-1)) AFP levels. This method can also be applied in serum samples, and also avoid the problem of particle aggregation caused by multiple separation and washing steps.

KeywordWashing-free Proximity Chemiluminescence Immunoassay Immunosensor Fe3o4
DOI10.1016/j.microc.2019.03.064
Language英语
WOS KeywordAlpha-fetoprotein ; Chemiluminescence Detection ; Cancer Statistics ; Immunoassay ; Serum ; Nanoparticles ; Binding ; Assay ; Dna
Funding ProjectNational Key Research and Development Program of China[2018YFF0214900]
WOS Research AreaChemistry
WOS SubjectChemistry, Analytical
Funding OrganizationNational Key Research and Development Program of China
WOS IDWOS:000468714400100
PublisherELSEVIER SCIENCE BV
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/29792
Collection中国科学院过程工程研究所
Corresponding AuthorGuan, Yueping
Affiliation1.Univ Sci & Technol Beijing, Sch Mat Sci & Engn, Beijing 100083, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, Key Lab Green Proc & Engn, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Zhi,Guan, Yueping,Xu, Guoli,et al. A new washing-free immunosensor for tumor marker detection based on functionalized Fe3O4 submicron particles[J]. MICROCHEMICAL JOURNAL,2019,147:824-831.
APA Zhang, Zhi,Guan, Yueping,Xu, Guoli,&Guo, Chen.(2019).A new washing-free immunosensor for tumor marker detection based on functionalized Fe3O4 submicron particles.MICROCHEMICAL JOURNAL,147,824-831.
MLA Zhang, Zhi,et al."A new washing-free immunosensor for tumor marker detection based on functionalized Fe3O4 submicron particles".MICROCHEMICAL JOURNAL 147(2019):824-831.
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